Article
Plant Sciences
Ayako Maeda, Sachi Takenaka, Tenghua Wang, Brody Frink, Toshiharu Shikanai, Mizuki Takenaka
Summary: C-to-U RNA editing sites in plant organelles exhibit a strong preference for neighboring nucleotides. The PPR domain of RNA editing factors binds to the 5' sequence of target cytidines, while the DYW domain catalyzes the C-to-U deamination. The DYW domain has a distinct preference for neighboring nucleotides, contributing to target selection.
Article
Plant Sciences
Xin-Yuan Liu, Rui-Cheng Jiang, Yong Wang, Jiao-Jiao Tang, Feng Sun, Yan-Zhuo Yang, Bao-Cai Tan
Summary: ZmPPR26 is essential for the C-to-U RNA editing and chloroplast biogenesis in maize. Loss of function of ZmPPR26 abolishes editing efficiency, leading to a decrease in chloroplast ATP synthase abundance and accumulation of photosynthetic complexes, ultimately resulting in the albino seedling-lethal phenotype.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Chemistry, Physical
Mizuki Takenaka, Sachi Takenaka, Tatjana Barthel, Brody Frink, Sascha Haag, Daniil Verbitskiy, Bastian Oldenkott, Mareike Schallenberg-Rudinger, Christian G. Feiler, Manfred S. Weiss, Gottfried J. Palm, Gert Weber
Summary: RNA editosomes selectively deaminate cytidines to uridines to restore protein functionality in plant organellar transcripts; DYW domains contain a cytidine deaminase fold and a C-terminal DYW motif, regulated by a gated zinc shutter in the deaminase fold.
Article
Biochemistry & Molecular Biology
Yan Zhao, Wei Xu, Yongzhong Zhang, Shilei Sun, Lijing Wang, Shiyi Zhong, Xiangyu Zhao, Baoshen Liu
Summary: Chloroplasts are crucial for plant growth and development, and the mutation of the PPR647 gene disrupts the expression of chloroplast genes, leading to defects in chloroplast development and abnormal plant growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Xingxing Feng, Suxin Yang, Yaohua Zhang, Cheng Zhiyuan, Kuanqiang Tang, Guang Li, Hui Yu, Jiantian Leng, Qingyu Wang
Summary: The study identified a Glycine max pale green leaf 2 mutant with decreased chlorophyll contents, caused by a single-nucleotide deletion in the GmPGL2 gene leading to a truncated protein. GmPGL2 participates in C-to-U RNA editing in soybean chloroplasts by interacting with various proteins to form a complex RNA editosome.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yong Wang, Xin-Yuan Liu, Zi-Qin Huang, Yan-Yan Li, Yan-Zhuo Yang, Aqib Sayyed, Feng Sun, Zhi-Qun Gu, Xiaomin Wang, Bao-Cai Tan
Summary: The study revealed the crucial role of EMP17 in C-to-U editing at multiple sites in mitochondrial transcripts, complex III biogenesis, and seed development in maize.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Yanwei Wang, Yu Duan, Pengfei Ai
Summary: In this study, the role of the OsTHA8 gene in chloroplast development in rice was investigated. OsTHA8 was found to be crucial for RNA editing and chloroplast biogenesis, shedding light on the regulatory mechanisms of chloroplast development.
Article
Biochemistry & Molecular Biology
Xiuming Li, Wenzhen Luo, Wen Zhou, Xiaopeng Yin, Xuemei Wang, Xiujin Li, Chenchen Jiang, Qingqing Zhang, Xiaojing Kang, Aihong Zhang, Yi Zhang, Congming Lu
Summary: The study revealed the importance of PPR proteins in stabilizing RNA transcripts by binding to them and inhibiting RNA endonuclease activity, thereby modulating gene expression levels in chloroplasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sachiko Toma-Fukai, Yuto Sawada, Ayako Maeda, Hikaru Shimizu, Toshiharu Shikanai, Mizuki Takenaka, Toshiyuki Shimizu
Summary: The activation mechanism of DYW1-like proteins is achieved through complementation of the DYW domain and reconstitution of the substrate-binding pocket. This provides structural insight into the activation mechanism of cytidine deaminase.
Article
Plant Sciences
Yingying Yang, Bastian Oldenkott, Shyam Ramanathan, Elena Lesch, Mizuki Takenaka, Mareike Schallenberg-Ruedinger, Volker Knoop
Summary: Protein factors for specific RNA editing events in plant mitochondria and chloroplasts have unique RNA-binding PPRs and DYW domains. The function of E1 and E2 motifs in these factors is unclear. In this study, the tolerance of PPR56 and PPR65 for creating chimeras by replacing their C-terminal protein regions was investigated. Results showed that heterologous DYW domains can restrict or widen the spectrum of off-targets in the bacterial transcriptome for PPR56, possibly due to their preference for the immediate sequence of the editing site and long-range impact on upstream PPRs' nucleotide selectivity.
Article
Agriculture, Multidisciplinary
Chang-zhao Chen, Ya-liang Wang, Meng-xing He, Zhi-wen Li, Lan Shen, Qing Li, De-Yong Ren, Jiang Hu, Li Zhu, Guang-heng Zhang, Zhen-yu Gao, Da-li Zeng, Long-biao Guo, Qian Qian, Qiang Zhang
Summary: Photosynthesis mainly occurs in chloroplasts, regulated by proteins encoded by nuclear genes. Among them, PPR proteins participate in organelle RNA editing. This study evaluated the functions of OsPPR9 in chloroplast RNA editing in rice.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Hiroshi Yamamoto, Nozomi Sato, Toshiharu Shikanai
Summary: The chloroplast NADH dehydrogenase-like (NDH) complex plays a crucial role in the electron transport chain in plants. The assembly and stability of subcomplex A (SubA) is dependent on the biosynthesis of the NdhA protein. Knockout mutants of ndhA in tobacco plants revealed the essential role of NdhA in stabilizing specific subunits of the NDH complex.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aidi Zhang, Yuhong Xiong, Fang Liu, Xiujun Zhang
Summary: This study analyzed the PPR gene family in kiwifruit and found that PPRs play important roles in fruit development and disease resistance. Two PPR genes potentially involved in fruit development and maturation were identified, and differential expression of PPRs and RNA editing was observed between resistant and susceptible kiwifruits following infection with pathogenic bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Elena Lesch, Maximilian T. Schilling, Sarah Brenner, Yingying Yang, Oliver J. Gruss, Volker Knoop, Mareike Schallenberg-Ruedinger
Summary: RNA editing processes differ significantly between animals and plants, with plant PPR proteins playing a key role in plant organelle cytidine-to-uridine editing. It has been discovered that plant PPR editing factors can operate in the nucleo-cytosolic environment of humans and be re-engineered towards new targets.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Genetics & Heredity
Tengfei Qin, Pei Zhao, Jialiang Sun, Yuping Zhao, Yaxin Zhang, Qiuyue Yang, Weipeng Wang, Zhuanqing Chen, Tengfei Mai, Yingying Zou, Guoxiang Liu, Wei Hao
Summary: RNA editing is a posttranscriptional phenomenon that primarily occurs in the genomes of mitochondria and chloroplasts in higher plants. Pentatricopeptide repeat (PPR) proteins, identified as potential trans-acting factors of RNA editing, have attracted attention in molecular biology research in recent years. The molecular functions and physiological roles of PPR proteins throughout plant growth and development are extensively studied.
FRONTIERS IN GENETICS
(2021)