Journal
FEBS LETTERS
Volume 588, Issue 21, Pages 3932-3938Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.09.005
Keywords
Plasma membrane; Inositiol phosphosphingolipid; phospholipase C (Isc1); Plasma membrane ATPase (Pma1); Sphingolipid; Cryptococcus neoformans
Funding
- NIH [AI56168, AI71142, AI87541, AI100631]
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Cryptococcus neoformans is a facultative intracellular pathogen, which can replicate in the acidic environment inside phagolysosomes. Deletion of the enzyme inositol-phosphosphingolipid-phospholipase-C (Isc1) makes C. neoformans hypersensitive to acidic pH likely by inhibiting the function of the proton pump, plasma membrane ATPase (Pma1). In this work, we examined the role of Isc1 on Pma1 transport and oligomerization. Our studies showed that Isc1 deletion did not affect Pma1 synthesis or transport, but significantly inhibited Pma1 oligomerization. Interestingly, Pma1 oligomerization could be restored by supplementing the medium with phytoceramide. These results offer insight into the mechanism of intracellular survival of C. neoformans. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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