Journal
FEBS LETTERS
Volume 588, Issue 24, Pages 4761-4768Publisher
WILEY
DOI: 10.1016/j.febslet.2014.11.008
Keywords
miR-96; Osteogensis; Differentiation; Heparin-binding epidermal growth factor-like growth factor
Funding
- National Natural Science Foundation of China [81071497]
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MicroRNAs (miRNAs) are a class of small non-coding RNAs with important roles in various biological and pathological processes, including osteoblast differentiation. Here, we identified miR-96 as a positive regulator of osteogenic differentiation in a mouse osteoblastic cell line (MC3T3-E1) and in mouse bone marrow-derived mesenchymal stem cells. Moreover, we found that miR-96 down-regulates post-transcriptional expression of heparin-binding EGF-like growth factor (HB-EGF) by specifically binding to the 3'untranslated region of HB-EGF mRNA. Furthermore, in MC3T3-E1 cells, miR-96-induced HB-EGF down-regulation suppressed the phosphorylation of epidermal growth factor receptor (EGFR) and of extracellular signal-regulated kinase 1 (ERK1) and AKT, which both lie downstream of EGFR activation. Taken together, miR-96 promotes osteogenic differentiation by inhibiting HB-EGF and by blocking the HB-EGF-EGFR signaling pathway in osteoblastic cells. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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