Review
Cell Biology
Nariaki Nakamura, Xiaobing Shi, Radbod Darabi, Yong Li
Summary: Cellular reprogramming is a crucial topic in stem cell and molecular biology research, involving aspects such as cell proliferation, determination of cell fate, and stem cell renewal. Factors like hypoxia and epigenetics play significant roles in tissue and organ development during this process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Hongli Jiao, Ming-Song Lee, Athillesh Sivapatham, Ellen M. Leiferman, Wan-Ju Li
Summary: This study successfully derived iPSCs from different breeds of miniature pigs and found that YMS fibroblasts showed higher reprogramming efficiency. The study also demonstrated the differentiation potential of YMS iPSC lines with a normal pig karyotype and identified the regulation of BAF60A expression by STAT3 signaling as a determinant of pig iPSC generation efficiency.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Md Emon Cevallos, Ricardo Raul Cevallos, Ruowen Zhang, Kejin Hu
Summary: Research has found that the generation of induced pluripotent stem cells (iPSCs) is inefficient and stochastic. The reasons for these deficiencies are not yet clear. It has been shown that the reprogramming factors, OCT4, SOX2, and KLF4, induce significant reprogramming stress, including transcriptional turbulence, stress-response gene deregulation, cell cycle impairment, downregulation of mitotic genes, illegitimate reprogramming, and cytotoxicity. However, the dominant-negative peptides of BET proteins enhance iPSC reprogramming and alleviate these reprogramming stresses. These peptides target a similar set of genes as BET chemical inhibitors, suggesting a distinct approach to targeting BET proteins.
Article
Multidisciplinary Sciences
Kuniyuki Aso, Michihito Kono, Masatoshi Kanda, Yuki Kudo, Kodai Sakiyama, Ryo Hisada, Kohei Karino, Yusho Ueda, Daigo Nakazawa, Yuichiro Fujieda, Masaru Kato, Olga Amengual, Tatsuya Atsumi
Summary: The study reveals that itaconate can alter T cell function and impact the development of autoimmune diseases through modulation of metabolic and epigenetic programs. Itaconate inhibits Th17 cell differentiation and promotes Treg cell differentiation by suppressing glycolysis and oxidative phosphorylation. The metabolic changes induced by itaconate also affect chromatin accessibility, transcription factor binding, and gene expression in Th17 and Treg cell differentiation. Furthermore, the adoptive transfer of itaconate-treated Th17 cells can ameliorate autoimmune encephalomyelitis. These findings suggest that itaconate is a crucial metabolic regulator for Th17/Treg cell balance and can be a potential therapeutic agent for autoimmune diseases.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Eva Hoermanseder
Summary: The stability of cell fates during development involves differentiation, cell memory, and the potential for reprogramming. Epigenetic mechanisms play a crucial role in maintaining stable cell-fate memory and resistance to reprogramming, highlighting the importance of understanding these processes in regenerative medicine.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2021)
Article
Biochemical Research Methods
Jennifer Hammelman, Tulsi Patel, Michael Closser, Hynek Wichterle, David Gifford
Summary: This study compares nine computational methods for identifying reprogramming factors for cell differentiation. The researchers found that methods using chromatin accessibility performed the best, with an average identification rate of 50-60% of reprogramming factors in the top ten candidates. Among the chromatin accessibility methods, DeepAccess and diffTF showed the highest correlation with the ranked significance of transcription factor candidates. AME and diffTF were identified as optimal methods for transcription factor recovery, allowing for systematic prioritization of candidates in the design of new reprogramming protocols.
Article
Multidisciplinary Sciences
Koki Yoshida, Osamu Uehara, Yoshihito Kurashige, Durga Paudel, Aya Onishi, Puja Neopane, Daichi Hiraki, Tetsuro Morikawa, Fumiya Harada, Rie Takai, Jun Sato, Masato Saitoh, Yoshihiro Abiko
Summary: The combination of a DNA demethylating agent and a histone deacetylase inhibitor can enhance the dedifferentiation of Malassez epithelial cell rests into stem-like cells, with the ability to differentiate into mesenchymal cells. This suggests a potential use for epigenetic agents in promoting periodontal tissue regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Sophia M. Hochrein, Hao Wu, Miriam Eckstein, Laura Arrigoni, Josip S. Herman, Fabian Schumacher, Christian Gerecke, Mathias Rosenfeldt, Dominic Gru, Burkhard Kleuser, Georg Gasteiger, Wolfgang Kastenmu, Bart Ghesquiere, Jan Van den Bossche, E. Dale Abel, Martin Vaeth
Summary: Metabolic reprogramming, specifically glycolytic reprogramming, plays a crucial role in activated T cells by regulating gene expression and pathogenicity. GLUT3 is essential for the effector functions of Th17 cells and its inhibition shows promise in mitigating Th17-cell-mediated inflammatory diseases.
Review
Cell Biology
Yuanjie Ding, Yuanchun Yao, Xingmu Gong, Qi Zhuo, Jinhua Chen, Miao Tian, Maryam Farzaneh
Summary: JMJD3 is a key histone demethylase that regulates H3K27me3 and plays a crucial role in stem cell fate determination during the process of cell reprogramming.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Chemistry, Physical
Anish Hiresha Verma, Rupa Haldavnekar, Krishnan Venkatakrishnan, Bo Tan
Summary: This study reports the fabrication of a 3D-silica nanostructured extracellular matrix for rapid reprogramming (RR) of tumor cells into cancer stem cells (CSCs) and in situ monitoring. The results show enhanced expressions of surface and epigenetic biomarkers, as well as the dual functionality of photodynamic monitoring.
Article
Medicine, Research & Experimental
Laura R. Bohrer, Nicholas E. Stone, Nathaniel K. Mullin, Andrew P. Voigt, Kristin R. Anfinson, Jessica L. Fick, Viviane Luangphakdy, Bradley Hittle, Kimerly Powell, George F. Muschler, Robert F. Mullins, Edwin M. Stone, Budd A. Tucker
Summary: In this study, the use of the Cell X precision robotic cell culture platform for parallel production of clinical grade patient-specific iPSCs was described. The iPSCs generated using the Cell X platform were pluripotent and genetically stable, and gave rise to retinal organoids that were indistinguishable from manually generated iPSCs. Single-cell RNA sequencing analysis showed that cells generated using the Cell X platform were comparable to those generated under manual conditions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Physical
Yang Song, Jennifer Soto, Binru Chen, Tyler Hoffman, Weikang Zhao, Ninghao Zhu, Qin Peng, Longwei Liu, Chau Ly, Pak Kin Wong, Yingxiao Wang, Amy C. Rowat, Siavash K. Kurdistani, Song Li
Summary: Deformation of the cell nucleus boosts cell reprogramming and offers potential applications in cell engineering.
Article
Oncology
Sakshi Goel, Vipul Bhatia, Tanay Biswas, Bushra Ateeq
Summary: The epigenetic landscape plays a crucial role in cell fate, differentiation, and prostate cancer progression, and research suggests its significance in the epigenetic reprogramming in cancer.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biology
Joao C. Sabino, Madalena R. de Almeida, Patricia L. Abreu, Ana M. Ferreira, Paulo Caldas, Marco M. Domingues, Nuno C. Santos, Claus M. Azzalin, Ana Rita Grosso, Sergio Fernandes de Almeida
Summary: The activity of TET enzymes promotes the formation of R-loops, revealing new mechanisms of gene expression regulation.
Article
Cell & Tissue Engineering
Yuta Tsujisaka, Takeshi Hatani, Chikako Okubo, Ryo Ito, Azuma Kimura, Megumi Narita, Kazuhisa Chonabayashi, Shunsuke Funakoshi, Antonio Lucena-Cacace, Taro Toyoda, Kenji Osafune, Takeshi Kimura, Hirohide Saito, Yoshinori Yoshida
Summary: By combining miR-switch with MACS technology, we have developed a rapid and efficient method for purifying large amounts of PSC-derived cells. This method can detect specific miRNAs in target cells and use CD4 transgene as a selection marker for MACS, achieving cell purification.
