Article
Veterinary Sciences
Yuki Yoshimura, Kazuomi Nakamura, Misako Seno, Misa Mochizuki, Kenji Kawai, Satoshi Koba, Tatsuo Watanabe
Summary: c-Fos is a useful marker gene of neuron activation for neuroscience and physiology research. Although c-Fos reporter knock-in (KI) mice have been used to investigate the mechanism and function of neural networks, performing surgical procedures on specific brain regions is challenging due to the small size of the mice. In contrast, rats have been extensively used in behavioral studies. This study successfully generated c-Fos-deficient rats using CRISPR/Cas, and observed similar abnormalities as observed in c-Fos knockout (KO) mice. The findings suggest that c-Fos gene in rats is expected to have the same function as that in mice.
EXPERIMENTAL ANIMALS
(2023)
Article
Environmental Sciences
Caiyun Lai, Fan Wu, Yan Wang, Wei Wang, Yueqi Li, Gaoqiang Zhang, Jianji Gao, Zhiliang Zhu, Jianhui Yuan, Jianping Yang, Wenjuan Zhang
Summary: TCE exposure can cause DNA hypomethylation and histone hyperacetylation in cells, leading to increased protein levels of tumor-related genes and inhibiting cell growth. These changes could serve as early biomarkers for TCE-associated diseases.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Cell Biology
Raquel Garcia-Lopez, Ana Pombero, Alicia Estirado, Emilio Geijo-Barrientos, Salvador Martinez
Summary: Our study suggests that deleting the first coding exon of the Lis1 gene may cause cortical anomalies associated with the pathophysiology of schizophrenia. The Lis1/sLis1 murine model shows abnormal neuronal morphology and enhanced cortical excitability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
James T. Leech, Andrew Brennan, Nicola A. Don, Jody M. Mason, Neil M. Kad
Summary: The AP-1 transcription factor family plays a crucial role in regulating the cell cycle and has various functions in proliferation, differentiation, and stress response. This study shows that cFos can bind to DNA independently of cJun and can interact with DNA as both monomers and dimers.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Neurosciences
Ji Won Yoo, Sung-Hwa Sohn, Yeon-Hwa Kim, Too Jae Min
Summary: This study demonstrated that UTI improved learning and memory in zebrafish by enhancing the expression levels of glutamate transporter and cognitive bio-markers. The dose-dependent effect of UTI was observed, indicating that UTI-enhanced learning and memory in zebrafish.
NEUROMOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Sung Shin Ahn, Hyunjin Yeo, Euitaek Jung, Yoongho Lim, Young Han Lee, Soon Young Shin
Summary: This study identified the molecular mechanisms by which inflammatory cytokines inhibit the expression of FLG in the skin. It was found that the formation of the FRA1:c-JUN:HDAC1 complex is promoted under TNFa + IFNγ stimulation, leading to the downregulation of FLG expression. This insight into the regulatory pathways of FLG expression provides potential therapeutic strategies for restoring skin barrier function in chronic skin inflammation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ahmed M. Sayed, Maha M. Abdel-Fattah, Hany H. Arab, Wafaa R. Mohamed, Emad H. M. Hassanein
Summary: Perindopril attenuated MTX-induced intestinal injury by suppressing TLR4/NF-kappa B and c-Fos/c-Jun pathways, enhancing PPAR-gamma/SIRT1 cytoprotective signals, decreasing expression of inflammatory cytokines, and increasing anti-inflammatory factors.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Xiaodong Zhang, Fangyu Hu, Baiwang Zhu, Xueli Jiao, Yun Li, Shuang Wu, Ganglin Ren, Jizhen Li, Qipeng Xie, Yifei Pan, Hongyan Li, Lingling Zhao
Summary: This study investigates the role of TEX11 gene in colorectal cancer (CRC). The results show that TEX11 expression is significantly downregulated in clinical samples and cell lines, and is associated with poor prognosis in CRC patients. Further experiments demonstrate that TEX11 can inhibit the proliferative capacity of CRC cells. Mechanistically, TEX11 promotes COP1 transcription by upregulating FOXO3a expression, thus accelerating the degradation of the negative transcriptional regulator c-Jun and enhancing p21 transcription, leading to inhibition of CRC cell proliferation.
Article
Biochemistry & Molecular Biology
Johanna Horberg, Kevin Moreau, Markus J. Tamas, Anna Reymer
Summary: The study demonstrates that AP-1 factors actively utilize the sequence-specific DNA flexibility to form stable protein-DNA complexes, while also being modulated by adjacent nucleotides to influence gene expression levels.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Jiseok Lee, Joanna Urban-Ciecko, Eunsol Park, Mo Zhu, Stephanie E. Myal, David J. Margolis, Alison L. Barth
Summary: The expression of immediate-early genes (IEGs) has been utilized to pinpoint small neural ensembles associated with specific experiences, however, in whisker-dependent sensory association learning, the training effect on IEG-marked neurons is not detectable. Synaptic strengthening in response to training is concentrated in non-IEG marked neurons in the primary somatosensory cortex.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Dariusz Wypior, Miroslaw Klinkowski, Igor Michalski
Summary: The Open RAN movement is expected to revolutionize network deployments and supply chains with the introduction of tailor-made solutions. O-RAN networks have already provided broadband services, and there is ongoing research in academia and industry on the potential benefits and market trends of open RAN.
APPLIED SCIENCES-BASEL
(2022)
Review
Immunology
Dandan Song, Yan Lian, Lin Zhang
Summary: AP-1 is a transcription factor involved in cell proliferation, migration, and invasion. Dysfunctional AP-1 activity is associated with cancer initiation, development, invasion, migration, and drug resistance. Small molecule inhibitors targeting AP-1 have shown some anticancer effects, but more research is needed to understand the specific mechanisms and select appropriate inhibitors and treatment strategies. Ultimately, this review summarizes the potential of combination therapy for cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Urology & Nephrology
Ronak Lakhia, Abheepsa Mishra, Laurence Biggers, Venkat Malladi, Patricia Cobo-Stark, Sachin Hajarnis, Vishal Patel
Summary: This study found elevated levels of H3K27ac, an acetylated modification of the DNA packing protein histone H3 that marks active enhancers, in polycystic kidney disease (PKD). Through comparative analysis, the researchers mapped over 16000 active intronic and intergenic enhancer elements in the kidneys of Pkd1-mutant mice. They also identified enhancer clusters that coexist with activated genes in PKD models, and deleting these regulatory elements suppressed cell proliferation and cyst growth. Furthermore, inhibiting glycolysis and glutaminolysis or activating Ppara reduced global H3K27ac levels and abundance on c-Myc enhancers. Therefore, epigenetic rewiring may play a role in the dysregulation of gene expression in PKD, and targeting the regulatory elements could potentially slow cyst growth.
KIDNEY INTERNATIONAL
(2022)
Article
Neurosciences
Yan Fu, Liting Sun, Fengting Zhu, Wei Xia, Ting Wen, Ruilong Xia, Xin Yu, Dan Xu, Changgeng Peng
Summary: Neuropathic pain caused by spinal cord injury is a long-term disturbance for patients, and the underlying mechanisms are still unclear. This study found that the abnormal expression of Nav1.7 in SDH is associated with NP. Pharmacological studies demonstrated that the blood-brain-barrier permeable Nav1.7 inhibitor GNE-0439 has a significant analgesic effect on NP in SCI mice.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Evolutionary Biology
Rabi Murad, Aide Macias-Munoz, Ashley Wong, Xinyi Ma, Ali Mortazavi
Summary: The study reveals that Hydra has complex gene regulatory structures, with dynamic remodeling of regulatory elements during head regeneration and enrichment of a specific set of transcription factor motifs in activated enhancer regions. This suggests that the evolution of complex developmental enhancers may predate the split of cnidarians and bilaterians.
GENOME BIOLOGY AND EVOLUTION
(2021)
