Journal
FEBS LETTERS
Volume 584, Issue 1, Pages 166-172Publisher
WILEY
DOI: 10.1016/j.febslet.2009.11.044
Keywords
FtsZ; Electron microscopy; Self-assembly; GTP; Plastid division; Arabidopsis thaliana
Funding
- OVPR
Ask authors/readers for more resources
Plants and algae contain the FtsZ1 and FtsZ2 protein families that perform specific, non-redundant functions in plastid division. In vitro studies of chloroplast division have been hampered by the lack of a suitable expression system. Here we report the expression and purification of FtsZ1-1 and FtsZ2-1 from Arabidopsis thaliana using a eukaryotic host. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. The purified proteins readily assembled into previously unreported assembly products named type-I and -II. laments. In contrast to bacterial FtsZ, the Arabidopsis proteins do not form bundled sheets in the presence of Ca2+. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available