Journal
FEBS LETTERS
Volume 583, Issue 9, Pages 1463-1468Publisher
WILEY
DOI: 10.1016/j.febslet.2009.03.060
Keywords
Adipokinetic hormone; Signal transduction; Receptor; G protein; Internalization; Mitogen-activated protein kinase; Bombyx mori
Funding
- National Natural Science Foundation of China [30670425]
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Neuropeptides of the adipokinetic hormone (AKH) family are among the best studied hormone peptides, but its signaling pathways remain to be elucidated. In this study, we molecularly characterized the signaling of Bombyx AKH receptor (AKHR) and its peptide ligands in HEK293 cells. In HEK293 cells stably expressing AKHR, AKH1 stimulation not only led to a ligand concentration dependent mobilization of intracellular Ca2+ and cAMP accumulation, but also elicited transient activation of extracellular signal-regulated kinase 1/2 (ERK1/2) pathway. We observed that AKH receptor was rapidly internalized after AKH1 stimulation. We further demonstrated that AKH2 exhibited high activities in cAMP accumulation and ERK1/2 activation on AKHR comparable to AKH1, whereas AKH3 was much less effective. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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