Article
Multidisciplinary Sciences
Qianhua Dong, Xue-lei Liu, Xiao-hui Wang, Yu Zhao, Yu-hang Chen, Fei Li
Summary: CENP-T interacts with Ccp1 to regulate centromere binding throughout the cell cycle, involving phosphorylation of a specific domain CIM. Competitive exclusion between Ccp1 and Ndc80 at the N terminus of CENP-T via phosphorylation ensures precise kinetochore assembly during mitosis, uncovering a previously unrecognized mechanism for kinetochore assembly through the cell cycle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Liqiao Hu, Congcong Zhao, Mingjie Liu, Shuaiyu Liu, Jingjing Ye, Kehui Wang, Jinyun Shi, Wei Tian, Xiaojing He
Summary: CENP-I directly interacts with centromeric DNA and stabilizes the deposition of CENP-A. The DNA binding of CENP-I is crucial for the loading of CENP-A and the localization of centromeres.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Harsh Nagpal, Beat Fierz
Summary: The centromere is an indispensable chromatin domain in eukaryotes, necessary for chromosome segregation; its unique structure relies on nucleosomes containing CENP-A; CENP-A binding proteins play a crucial role in modulating the structure of centromeric chromatin.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Guojon Olafsson, Max A. B. Haase, Jef D. Boeke
Summary: This study demonstrates that the differences in protein composition from different species may lead to complementation failure during mitosis.
G3-GENES GENOMES GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Verena Cmentowski, Giuseppe Ciossani, Ennio d'Amico, Sabine Wohlgemuth, Mikito Owa, Brian Dynlacht, Andrea Musacchio
Summary: The RZZS complex and CENP-E are closely connected and reciprocally required for their own kinetochore recruitment and dynein localization to the corona.
Review
Biochemistry & Molecular Biology
Marco A. Andonegui-Elguera, Rodrigo E. Caceres-Gutierrez, Alejandro Lopez-Saavedra, Fernanda Cisneros-Soberanis, Montserrat Justo-Garrido, Jose Diaz-Chavez, Luis A. Herrera
Summary: During mitosis, chromatin undergoes various activities regulated by histone post-translational modifications (HPTMs). Specific HPTMs play roles in chromosome condensation, centromere formation, and the assembly of regulatory protein complexes. Additionally, environmental factors can modify HPTMs, affecting chromosome segregation and genome stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Koei Okazaki, Megumi Nakano, Jun-ichirou Ohzeki, Koichiro Otake, Kazuto Kugou, Vladimir Larionov, William C. Earnshaw, Hiroshi Masumoto
Summary: Human artificial chromosomes (HACs) are formed by introducing large centromeric sequences into cells, and the balance of chromatin states on the alphoid DNA is crucial for HAC formation. Our study explores the relationship between chromatin architecture and de novo HAC formation efficiency, and found that a combination of mutated and wild-type alphoid repeats enhances HAC formation.
Review
Cell Biology
Charlene Renaud-Pageot, Jean-Pierre Quivy, Marina Lochhead, Genevieve Almouzni
Summary: CENP-A plays a critical role in chromosome segregation and genome integrity maintenance in mammals, but its overexpression is linked to cancer development. Studies have shown that CENP-A overexpression can affect genome integrity, cell fate, and nuclear organization in cancer, highlighting its potential as a target for cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Jessica R. Eisenstatt, Kentaro Ohkuni, Wei-Chun Au, Olivia Preston, Loran Gliford, Evelyn Suva, Michael Costanzo, Charles Boone, Munira A. Basrai
Summary: The mislocalization of overexpressed Cse4 is influenced by defects in proteolysis and sumoylation, with reduced dosage of histone H4 leading to suppression of CIN when Cse4 is overexpressed. This study identified genes that contribute to the mislocalization of Cse4 and highlighted the role of H4 in facilitating Cse4 sumoylation and mislocalization to noncentromeric regions.
Article
Cell Biology
Julio C. Flores C. Servin, Rachel R. Brown, Aaron F. Straight
Summary: Flores Servin, Brown, and Straight discovered that vertebrate CENP-A assembly is restricted to G1 phase due to the inhibitory activities of HJURP phosphorylation and M18BP1.S competitive binding to CENP-C. These inhibitory activities prevent the metaphase centromere localization of HJURP and thus hinder CENP-A assembly. Removal of these inhibitory activities allows CENP-A assembly in metaphase.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mariko Ariyoshi, Fumiaki Makino, Reito Watanabe, Reiko Nakagawa, Takayuki Kato, Keiichi Namba, Yasuhiro Arimura, Risa Fujita, Hitoshi Kurumizaka, Ei-ichi Okumura, Masatoshi Hara, Tatsuo Fukagawa
Summary: The study reveals a stable binding mode of CENP-C to the CENP-A nucleosome through unique regions, stabilized by an intramolecular link through a phosphorylated CENP-C residue. The stable CENP-A-CENP-C complex excludes CENP-N from the CENP-A nucleosome, providing mechanistic insights into the dynamic kinetochore assembly regulated by CDK1-mediated CENP-C phosphorylation.
Article
Genetics & Heredity
Na Wang, Jianing Liu, William A. Ricci, Jonathan Gent, R. Kelly Dawe
Summary: The research reveals a positive relationship between centromere size and genome size, independent of variations in major centromeric satellite sequences. Additionally, changes in centromere size involve alterations in bound CENH3, but overexpressing CENH3 does not mimic this effect. The study demonstrates that centromere size is among the scalable features affected by multiple limiting factors.
Review
Cell Biology
Alessandro Stirpe, Patrick Heun
Summary: Centromeres are specialized chromosome domains characterized by the epigenetic mark CENP-A. This review focuses on the establishment and maintenance of CENP-A during essential cellular processes, such as DNA repair, replication, and transcription, and highlights conserved mechanisms among different model organisms. It discusses the coordination and control of timely loading, maintenance, and removal of CENP-A and the gradually unraveled network of factors involved.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Fioranna Renda, Alexey Khodjakov
Summary: The kinetochore plays a crucial role in facilitating chromosome segregation during cell division by attaching chromosomes to spindle microtubules. It also acts as a signaling hub to regulate cell cycle progression and ensures the accuracy of chromosome segregation. Despite being large and robust molecular assemblies, kinetochores undergo dynamic structural and organizational changes throughout the cell cycle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Divine Mensah Sedzro, Xiao Yuan, McKay Mullen, Umer Ejaz, Tongtong Yang, Xu Liu, Xiaoyu Song, Yun-Chi Tang, Weijun Pan, Peng Zou, Xinjiao Gao, Dongmei Wang, Zhikai Wang, Zhen Dou, Xing Liu, Xuebiao Yao
Summary: The study reveals that CENP-R collaborates with CENP-OPQU to regulate the stability of kinetochore-microtubule attachment and ensure accurate chromosome segregation during mitosis. Phosphorylation of CENP-R affects its localization to the kinetochore, possibly by disrupting its binding with CENP-U.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Oncology
Gabriele Corda, Gianluca Sala, Rossano Lattanzio, Manuela Iezzi, Michele Sallese, Giorgia Fragassi, Alessia Lamolinara, Hasan Mirza, Daniela Barcaroli, Sibylle Ermler, Elisabete Silva, Hemad Yasaei, Robert F. Newbold, Paola Vagnarelli, Marcella Mottolese, Pier Giorgio Natali, Letizia Perracchio, Jelmar Quist, Anita Grigoriadis, Pierfrancesco Marra, Andrew N. Tutt, Mauro Piantelli, Stefano Iacobelli, Vincenzo De Laurenzi, Arturo Sala
JOURNAL OF PATHOLOGY
(2017)
Correction
Cell Biology
Carol-Anne Martin, Jennie E. Murray, Paula Carroll, Andrea Leitch, Karen J. MacKenzie, Mihail Halachev, Ahmed E. Fetit, Charlotte Keith, Louise S. Bicknell, Adeline Fluteau, Philippe Gautier, Emma A. Hall, Shelagh Joss, Gabriela Soares, Joao Silva, Michael B. Bober, Angela Duker, Carol A. Wise, Alan J. Quigley, Shubha R. Phadke, Andrew J. Wood, Paola Vagnarelli, Andrew P. Jackson
GENES & DEVELOPMENT
(2017)
Article
Multidisciplinary Sciences
Giulia Vargiu, Alexandr A. Makarov, James Allan, Tatsuo Fukagawa, Daniel G. Booth, William C. Earnshaw
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Review
Cell Biology
Daniel G. Booth, William C. Earnshaw
TRENDS IN CELL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Ines J. de Castro, James Budzak, Maria L. Di Giacinto, Lorena Ligammari, Ezgi Gokhan, Christos Spanos, Daniela Moralli, Christine Richardson, Jose I. de las Heras, Silvia Salatino, Eric C. Schirmer, Katharine S. Ullman, Wendy A. Bickmore, Catherine Green, Juri Rappsilber, Sarah Lamble, Martin W. Goldberg, Veronica Vinciotti, Paola Vagnarelli
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Nicholas C. O. Lee, Jung-Hyun Kim, Nikolai S. Petrov, Hee-Sheung Lee, Hiroshi Masumoto, William C. Earnshaw, Vladimir Larionov, Natalay Kouprina
ACS SYNTHETIC BIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Raquel Sales Gil, Ines J. de Castro, Jerusalem Berihun, Paola Vagnarelli
BIOCHEMICAL SOCIETY TRANSACTIONS
(2018)
Article
Biochemistry & Molecular Biology
Raquel Sales Gil, Paola Vagnarelli
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Johan H. Gibcus, Kumiko Samejima, Anton Goloborodko, Itaru Samejima, Natalia Naumova, Johannes Nuebler, Masato T. Kanemaki, Linfeng Xie, James R. Paulson, William C. Earnshaw, Leonid A. Mirny, Job Dekker
Editorial Material
Biochemistry & Molecular Biology
Paola Vagnarelli, Dario R. Alessi
Editorial Material
Biochemistry & Molecular Biology
Raquel Sales Gil, Paola Vagnarelli
TRENDS IN BIOCHEMICAL SCIENCES
(2018)
Review
Cell Biology
Florentin Huguet, Shane Flynn, Paola Vagnarelli
Meeting Abstract
Cell Biology
R. Sales Gil, H. Amin, V. Vinciotti, P. Vagnarelli
MOLECULAR BIOLOGY OF THE CELL
(2018)
Meeting Abstract
Cell Biology
E. Gokhan, P. Vagnarelli
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Cell Biology
I. J. de Castro, H. A. Amin, V. Vinciotti, P. Vagnarelli