Journal
FEBS LETTERS
Volume 583, Issue 2, Pages 419-424Publisher
WILEY
DOI: 10.1016/j.febslet.2008.12.040
Keywords
M1 RNA; C5 protein; RNase P; RNA stability
Funding
- 21C Frontier Microbial Genomics and Application Center Program [MG08-0201-2-0]
- Korea Science and Engineering Foundation [R01-2005-000-10293-0, R01-2008-000-11660-0]
- National Research Foundation of Korea [11-2008-06-002-00, 2008-0058476, R01-2005-000-10293-0, R01-2008-000-11660-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Escherichia coli RNase P is a ribonucleoprotein composed of a large RNA subunit (M1 RNA) and a small protein subunit (C5 protein). We examined if C5 protein plays a role in maintaining metabolic stability of M1 RNA. The sequestration of C5 protein available for M1 RNA binding reduced M1 RNA stability in vivo, and its reduced stability was recovered via overexpression of C5 protein. In addition, M1 RNA was rapidly degraded in a temperature-sensitive C5 protein mutant strain at non-permissive temperatures. Collectively, our results demonstrate that the C5 protein metabolically stabilizes M1 RNA in the cell. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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