Journal
FEBS LETTERS
Volume 582, Issue 28, Pages 3922-3928Publisher
WILEY
DOI: 10.1016/j.febslet.2008.10.036
Keywords
HAK; KUP; Potassium; Sodium; Transporter
Funding
- ANPCYT-PICT [20138/04]
- CONICET-PIP
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Members of group I KT-HAK-KUP transporters play an important role in K+ acquisition by plant roots, a process that is strongly affected by salt stress. A PCR-based random mutagenesis approach on HvHAK1 allowed identification of V366I and R591C substitutions, which confer enhanced K+-capture, and improved NaCl, LiCl and NH4Cl tolerance, to yeast cells. Improved K+-capture was linked to an enhanced Vmax. Results reveal an intrinsic protective effect of K+, and assign an important role to the 8th transmembrane domain, as well as the C-terminus, in determining the maximum capacity for the transport of K+ in KT-HAK-KUP transporters. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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