Article
Biochemistry & Molecular Biology
Aanchal Aggarwal, Aarti Yadav, Neetu Saini, Rajat Sandhir
Summary: This study evaluated the protective role of GSNO in preventing neurobehavioral deficits induced by hyperglycemia. GSNO supplementation ameliorated alterations in monoaminergic system and nitro-oxidative stress, restoring neurobehavioral functions.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Megan Pophal, Zachary W. Grimmett, Clara Chu, Seunghee Margevicius, Thomas Raffay, Kristie Ross, Anjum Jafri, Olivia Giddings, Jonathan S. Stamler, Benjamin Gaston, James D. Reynolds
Summary: The study suggests that F-ENO could reflect airway S-nitrosothiol concentrations and serve as a biomarker for asthma. Inhalational challenge with GSNO resulted in increased F-ENO, indicating a potential non-invasive test for monitoring airway GSNO breakdown.
Article
Biochemistry & Molecular Biology
Judong Kim, S. M. Touhidul Islam, Fei Qiao, Avtar K. Singh, Mushfiquddin Khan, Jeseong Won, Inderjit Singh
Summary: The research shows that regulating the cytokines released by B cells can protect against neuroinflammatory diseases like experimental autoimmune encephalomyelitis. Using GSNO and GSNOR inhibitor N6022 can modulate IL-10 and IL-6, offering potential in treating autoimmune disorders.
Article
Biochemistry & Molecular Biology
Nusrat Jahan Methela, Mohammad Shafiqul Islam, Da-Sol Lee, Byung-Wook Yun, Bong-Gyu Mun
Summary: Heavy metal toxicity, including lead (Pb) toxicity, is increasing in soils due to industrialization, agricultural practices, and urban practices. Pb contamination seriously damages crop growth and affects plant growth and development through photosystem interference and production of reactive oxygen species (ROS). Nitric oxide (NO) and S-nitrosoglutathione (GSNO) applications have shown positive effects in improving soybean growth under lead stress by reducing oxidative damage and lipid peroxidation. These applications also modulate ion homeostasis and alleviate oxidative damage caused by ROS scavenging. In summary, NO and GSNO applications are effective in detoxifying ROS induced by lead toxicity in soybean.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Guochen Qin, Menghuan Qu, Bei Jia, Wei Wang, Zhuojun Luo, Chun-Peng Song, W. Andy Tao, Pengcheng Wang
Summary: This study developed a highly sensitive method for detecting S-nitrosylation peptides, allowing for quantitative identification of S-nitrosylated proteins and revealing a key role of GSNOR1 in regulating endoplasmic reticulum functions in Arabidopsis.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Susmita Das, Zeeshan Ahmad, Sneha Singh, Sukhvinder Singh, Robert Emery Wright, Shailendra Giri, Ashok Kumar
Summary: In this study, the researchers found that S-nitroso-l-glutathione (GSNO) levels were reduced in an experimental murine Staphylococcus aureus (SA) endophthalmitis model. They tested the effects of GSNO supplementation via different routes and found that intravitreal injection of GSNO provided protection against SA endophthalmitis, while oral administration of GSNO was most effective in reducing inflammation and bacterial burden. In vitro experiments also showed that GSNO treatment reduced inflammatory mediators and cell death caused by SA, and strengthened the outer blood-retinal barrier during endophthalmitis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ajanta Chatterji, Rajib Sengupta
Summary: Nitric oxide is a gasotransmitter molecule that plays various roles in cellular processes. Recent research supports the existence of S-nitrosylated proteins within cells, challenging the controversy over their stability. Evidence suggests that these proteins directly regulate functionality and provide insights into cell signaling.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Syed Kashif Zaidi, Md Nasrul Hoda, Shams Tabrez, Mohammad Imran Khan
Summary: The restoration of cerebral blood flow (CBF) through remote ischemic conditioning (RIC) is a primary treatment for ischemic stroke. However, NOS3 dysfunction and diabetes may neutralize the benefits of RIC therapy in stroke, but can be turned protective in combination with GSNOR inhibitor (GRI). Our findings support the future clinical trial of RIC in comorbid stroke.
Review
Biochemistry & Molecular Biology
Francisco Javier Romera, Maria Jose Garcia, Carlos Lucena, Macarena Angulo, Rafael Perez-Vicente
Summary: Iron deficiency responses in plants involve various hormones and signaling molecules, including auxin, ethylene, glutathione (GSH), nitric oxide (NO), and S-nitrosoglutathione (GSNO). However, the regulation of these responses in dicot plants is not fully understood. This review provides an update on the role of NO, GSNO, and GSNOR in the regulation of Fe deficiency responses, discussing their interactions with auxin and ethylene and their mode of action through post-translational modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Yuhe Zhao, Yang Liu, Jianfen Wu, Dan Kong, Sifan Zhao, Guopan Li, Wei Li
Summary: The authors cloned the ALR gene from swamp eel and expressed and purified the recombinant protein. The rALR protein showed efficient reductive activity against various aldehydes, ketones, and GSNO. Administration of rALR in swamp eels increased hepatic GSH levels, decreased hepatic NO content and levels of reactive oxygen and nitrogen species, and regulated the expression of related genes, suggesting a role of ALR in response to nitrosative stress.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ajanta Chatterji, Debasmita Banerjee, Timothy R. Billiar, Rajib Sengupta
Summary: This article discusses the role of S-nitrosylation in cellular processes, as well as the importance of thioredoxin and S-nitrosoglutathione reductase in combating nitric oxide pathology and modulating inflammation.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Plant Sciences
Lin Zhang, Haiyan Song, Baohai Li, Meng Wang, Dongwei Di, Xianyong Lin, Herbert J. Kronzucker, Weiming Shi, Guangjie Li
Summary: High levels of NH4+ induce NO accumulation and inhibit K+ acquisition via SNO1/SOS4, leading to reduced root growth tolerance, while GSNOR plays a crucial role in improving root tolerance to NH4+ by counteracting NO-mediated suppression of tissue K+.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Editorial Material
Plant Sciences
Inmaculada Sanchez-Vicente, Oscar Lorenzo
Summary: Climate change affects plant growth with heat and cold waves, but identification of key signaling molecules like nitric oxide can help mitigate adverse effects and improve plant tolerance. NO is a central gasotransmitter in stress responses, enhancing plant adaptation to heat and cold stress and suggesting new approaches for future environmental challenges.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Plant Sciences
Baohai Li, Chengliang Sun, Xianyong Lin, Wolfgang Busch
Summary: Oxidative stress, a common occurrence in aerobic organisms, is a fundamental and unavoidable cost of aerobic lifestyle. GSNOR, a conserved enzyme in the GSNO metabolism, plays a crucial regulatory role in oxidative stress by modulating the interaction of antioxidants and iron to regulate stress responses in plants. Recent advancements in understanding GSNOR regulation show promise for the modulation of oxidative stress in plants.
TRENDS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Jiashen Zhou, Lin Zhang, Liping Zeng, Lu Yu, Yuanyuan Duan, Siqi Shen, Jingyan Hu, Pan Zhang, Wenyan Song, Xiaoxue Ruan, Jing Jiang, Yinan Zhang, Lu Zhou, Jia Jia, Xudong Hang, Changlin Tian, Houwen Lin, Hong-Zhuan Chen, John E. Cronan, Hongkai Bi, Liang Zhang
Summary: The study presents the crystal structures of the essential UFA biosynthesis enzyme FabX in Helicobacter pylori, revealing a unique [4Fe-4S] cluster that potentiates FMN oxidation during dehydrogenase catalysis. This [4Fe-4S] cluster is crucial for unsaturated fatty acid synthesis and contributes to the pathogenic function of H. pylori in the corrosion of gastric mucosa.
NATURE COMMUNICATIONS
(2021)
Article
Hematology
Pedro Goncalves, Jean-Marc Doisne, Toshiki Eri, Bruno Charbit, Vincent Bondet, Celine Posseme, Alba Llibre, Armanda Casrouge, Christelle Lenoir, Benedicte Neven, Darragh Duffy, Alain Fischer, James P. Di Santo
Summary: Both innate and adaptive lymphocytes play critical roles in mucosal defense. SCID patients receiving HSCT may have impaired lymphocyte regeneration. SCID patients with IL2RG/GC or JAK3 genetic defects exhibit defects in mucosal immunity and IgA production, leading to microbial dysbiosis. IVIG replacement therapy can partially restore these immune defects.
Review
Chemistry, Inorganic & Nuclear
Jordi Zamarreno Beas, Marco A. M. Videira, Ligia M. Saraiva
Summary: Haem b and sirohaem are two important prosthetic groups in bacterial proteins, synthesized through complex pathways with divergences at the formation of uroporphyrinogen III. The biosynthesis of these tetrapyrroles involves unstable or potentially hazardous intermediates and end-products, requiring tight control of cellular metabolic fluxes.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Jeremy Manry, Paul Bastard, Adrian Gervais, Tom Le Voyer, Jeremie Rosain, Quentin Philippot, Eleftherios Michailidis, Hans-Heinrich Hoffmann, Shohei Eto, Marina Garcia-Prat, Lucy Bizien, Alba Parra-Martinez, Rui Yang, Liis Haljasmagi, Melanie Migaud, Karita Sarekannu, Julia Maslovskaja, Nicolas de Prost, Yacine Tandjaoui-Lambiotte, Charles-Edouard Luyt, Blanca Amador-Borrero, Alexandre Gaudet, Julien Poissy, Pascal Morel, Pascale Richard, Fabrice Cognasse, Jesus Troya, Sophie Trouillet-Assant, Alexandre Belot, Kahina Saker, Pierre Garcon, Jacques G. Riviere, Jean-Christophe Lagier, Stephanie Gentile, Lindsey B. Rosen, Elana Shaw, Tomohiro Morio, Junko Tanaka, David Dalmau, Pierre-Louis Tharaux, Damien Sene, Alain Stepanian, Bruno Megarbane, Vasiliki Triantafyllia, Arnaud Fekkar, James R. Heath, Jose Luis Franco, Juan-Manuel Anaya, Jordi Sole-Violan, Luisa Imberti, Andrea Biondi, Paolo Bonfanti, Riccardo Castagnoli, Ottavia M. Delmonte, Yu Zhang, Andrew L. Snow, Steven M. Holland, Catherine M. Biggs, Marcela Moncada-Velez, Andres Augusto Arias, Lazaro Lorenzo, Soraya Boucherit, Dany Anglicheau, Anna M. Planas, Filomeen Haerynck, Sotirija Duvlis, Tayfun Ozcelik, Sevgi Keles, Ahmed A. Bousfiha, Jalila El Bakkouri, Carolina Ramirez-Santana, Stephane Paul, Qiang Pan-Hammarstrom, Lennart Hammarstrom, Annabelle Dupont, Alina Kurolap, Christine N. Metz, Alessandro Aiuti, Giorgio Casari, Vito Lampasona, Fabio Ciceri, Lucila A. Barreiros, Elena Dominguez-Garrido, Mateus Vidigal, Mayana Zatz, Diederik van de Beek, Sabina Sahanic, Ivan Tancevski, Yurii Stepanovskyy, Oksana Boyarchuk, Yoko Nukui, Miyuki Tsumura, Loreto Vidaur, Stuart G. Tangye, Sonia Burrel, Darragh Duffy, Lluis Quintana-Murci, Adam Klocperk, Nelli Y. Kann, Anna Shcherbina, Yu-Lung Lau, Daniel Leung, Matthieu Coulongeat, Julien Marlet, Rutger Koning, Luis Felipe Reyes, Angelique Chauvineau-Grenier, Fabienne Venet, Guillaume Monneret, Michel C. Nussenzweig, Romain Arrestier, Idris Boudhabhay, Hagit Baris-Feldman, David Hagin, Joost Wauters, Isabelle Meyts, Adam H. Dyer, Sean Kennelly, Nollaig M. Bourkeh, Rabih Halwan, Fatemeh Saheb Sharif-Askar, Karim Dorgham, Jerome Sallette, Souad Mehlal Sedkaoui, Suzan AlKhater, Raul Rigo-Bonnin, Francisco Morandeira, Lucie Roussel, Donald C. Vinh, Christian Erikstrup, Antonio Condino-Neto, Carolina Prando, Anastasiia Bondarenko, Andras N. Spaan, Laurent Gilardin, Jacques Fellay, Stanislas Lyonnet, Kaya Bilguvar, Richard P. Lifton, Shrikant Mane, Mark S. Anderson, Bertrand Boisson, Vivien Beziat, Shen-Ying Zhang, Evangelos Andreakos, Olivier Hermine, Aurora Pujol, Part Peterson, Trine H. Mogensen, Lee Rowen, James Mond, Stephanie Debette, Xavier de Lamballerie, Charles Burdet, Lila Bouadma, Marie Zins, Pere Soler-Palacin, Roger Colobran, Guy Gorochov, Xavier Solanich, Sophie Susen, Javier Martinez-Picado, Didier Raoult, Marc Vasse, Peter K. Gregersen, Lorenzo Piemonti, Carlos Rodriguez-Gallego, Luigi D Notarangelo, Helen C. Su, Kai Kisand, Satoshi Okada, Anne Puel, Emmanuelle Jouanguy, Charles M. Rice, Pierre Tiberghien, Qian Zhang, Jean-Laurent Casanova, Laurent Abel, Aurelie Cobat
Summary: This study investigates the impact of autoimmunity on COVID-19-related deaths and finds that individuals carrying autoantibodies neutralizing type I interferons are at higher risk of death, with the risk decreasing with age.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ilana Gabanyi, Gabriel Lepousez, Richard Wheeler, Alba Vieites-Prado, Antoine Nissant, Sebastien Wagner, Carine Moigneu, Sophie Dulauroy, Samia Hicham, Bernadette Polomack, Florine Verny, Philip Rosenstiel, Nicolas Renier, Ivo Gomperts Boneca, Gerard Eberl, Pierre-Marie Lledo
Summary: Gut bacteria can influence feeding behavior and body temperature control by directly sensing bacterial cell wall components through brain neurons.
Article
Oncology
Hirotaka Kawanabe-Matsuda, Kazuyoshi Takeda, Marie Nakamura, Seiya Makino, Takahiro Karasaki, Kazuhiro Kakimi, Megumi Nishimukai, Tatsukuni Ohno, Jumpei Omi, Kuniyuki Kano, Akiharu Uwamizu, Hideo Yagita, Ivo Gomperts Boneca, Gerard Eberl, Junken Aoki, Mark J. Smyth, Ko Okumura
Summary: This study demonstrated that dietary consumption of exopolysaccharide produced by Lactobacillus can induce CCR6(+) CD8(+) T cells in Peyer's patches, improving the tumor microenvironment and enhancing the therapeutic effects of immune-checkpoint blockade against CCL20-producing tumors.
Article
Genetics & Heredity
Barthelemy Caron, Etienne Patin, Maxime Rotival, Bruno Charbit, Matthew L. Albert, Lluis Quintana-Murci, Darragh Duffy, Antonio Rausell
Summary: This study evaluated plasma protein levels in a cohort of 400 unrelated healthy individuals of western European ancestry and identified novel genetic and non-genetic factors associated with plasma protein levels.
Article
Microbiology
S. Legood, D. Seng, I. G. Boneca, N. Buddelmeijer
Summary: This study demonstrates the essential role of Lgt, an enzyme in proteobacteria, in cell growth and viability. The absence of Lgt leads to severe growth and morphological defects that can be restored by expressing Lgt. It is also shown that Lpp is not the main cause of cell death under conditions of Lgt depletion, highlighting the importance of other lipoproteins in cell envelope biogenesis and cell viability.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Oncology
Anne-Gaelle Goubet, Leonardo Lordello, Carolina Alves Costa Silva, Isabelle Peguillet, Marianne Gazzano, Maxime Descartes Mbogning-Fonkou, Cassandra Thelemaque, Cedric Lebacle, Constance Thibault, Francois Audenet, Geraldine Pignot, Gwenaelle Gravis, Carole Helissey, Luca Campedel, Morgan Roupret, Evanguelos Xylinas, Idir Ouzaid, Agathe Dubuisson, Marine Mazzenga, Caroline Flament, Pierre Ly, Virginie Marty, Nicolas Signolle, Allan Sauvat, Thomas Sbarrato, Mounia Filahi, Caroline Davin, Gabriel Haddad, Jacques Bou Khalil, Camille Bleriot, Francois-Xavier Danlos, Garett Dunsmore, Kevin Mulder, Aymeric Silvin, Thibault Raoult, Baptiste Archambaud, Shaima Belhechmi, Ivo Gomperts Boneca, Nadege Cayet, Maryse Moya-Nilges, Adeline Mallet, Romain Daillere, Etienne Rouleau, Camelia Radulescu, Yves Allory, Jacques Fieschi, Mathieu Rouanne, Florent Ginhoux, Gwenael Le Teuff, Lisa Derosa, Aurelien Marabelle, Jeroen Van Dorp, Nick Van Dijk, Michiel S. Van der Heijden, Benjamin Besse, Fabrice Andre, Miriam Merad, Guido Kroemer, Jean-Yves Scoazec, Laurence Zitvogel, Yohann Loriot
Summary: CD4+ T cells and PD-L1 expression are correlated with the efficacy of immunotherapy drugs, which is important for the treatment of bladder cancer patients.
Article
Cell Biology
Celine Posseme, Alba Llibre, Bruno Charbit, Vincent Bondet, Vincent Rouilly, Violaine Saint-Andre, Jeremy Boussier, Jacob Bergstedt, Nikaia Smith, Liam Townsend, Jamie A. Sugrue, Cliona Ni Cheallaigh, Niall Conlon, Maxime Rotival, Michael S. Kobor, Estelle Mottez, Stanislas Pol, Etienne Patin, Matthew L. Albert, Lluis Quintana-Murci, Darragh Duffy
Summary: We found that interferon beta is a major determinant of IL-12p70 production induced by lipopolysaccharide and is associated with circulating monocytes. Reduced IFN beta-IL-12p70 responses were observed in patients with SARS-CoV-2 infection or chronic HCV infection, and these responses were resolved after viral clearance. This study provides important insights into the roles of IL-12p70 and IFN beta in healthy and infected individuals and how genetic and epigenetic variation may impact immune responses to bacterial infection.
Article
Immunology
Ignacio Santecchia, Delphine Bonhomme, Stylianos Papadopoulos, Pedro Escoll, Alexandre Giraud-Gatineau, Maryse Moya-Nilges, Frederique Vernel-Pauillac, Ivo Gomperts Boneca, Catherine Werts
Summary: This study reevaluated the intracellular fate of Leptospira interrogans and found that they actively enter macrophages but quickly exit without replicating inside the cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jacob Bergstedt, Sadoune Ait Kaci Azzou, Kristin Tsuo, Anthony Jaquaniello, Alejandra Urrutia, Maxime Rotival, David T. S. Lin, Julia L. MacIsaac, Michael S. Kobor, Matthew L. Albert, Darragh Duffy, Etienne Patin, Lluis Quintana-Murci
Summary: This study investigates the impact of DNA sequence variation, age, sex, as well as factors related to life habits and immunity on the DNA methylation of adult blood. It also explores whether these effects are mediated by changes in cellular composition. The results show significant differences in DNA methylation between naive and memory T cells, and reveal that latent cytomegalovirus infection drives DNA methylation variation. Epigenetic drift is identified as the cause of increased DNA methylation dispersion with aging. Cellular composition and DNA sequence variation are found to be the strongest predictors of DNA methylation.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Daniel Alvarez-Simon, Saliha Ait Yahia, Patricia de Nadai, Camille Audousset, Mathias Chamaillard, Ivo Gomperts Boneca, Anne Tsicopoulos
Summary: This review article discusses the expression, activation, and function of NLRs in relation to asthma and explores the potential interventions in NLR signaling as immunomodulatory therapies for asthma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Delphine Bonhomme, Veronica Hernandez-Trejo, Stylianos Papadopoulos, Remi Pigache, Martine Fanton d'Andon, Ahmed Outlioua, Ivo G. Boneca, Catherine Werts
Summary: Leptospira interrogans is capable of infecting all vertebrates and causing leptospirosis, but they do not trigger cell death in macrophages and escape pyroptosis and apoptosis. Instead, they induce mild IL-1b secretion through nonlytic caspase 8-dependent gasdermin D pore formation. Pathogenic L. interrogans prevent caspase 11 dimerization and gasdermin D cleavage, thus escaping massive cell death. This novel mechanism allows leptospires to evade inflammation and potentially contribute to their stealthiness.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Richard Wheeler, Paulo Andre Dias Bastos, Olivier Disson, Aline Rifflet, Ilana Gabanyi, Julia Spielbauer, Marion Berard, Marc Lecuit, Ivo Gomperts Boneca
Summary: Peptidoglycan, the major component of bacterial cell walls, plays a crucial role in affecting physiological and behavioral changes in mammalian hosts. However, the mechanism and regulation of its translocation from the gut to host tissues have not been fully understood. This study investigated the absorption and dissemination of peptidoglycan across the intestine. The results showed that peptidoglycan has a preference for certain host organs, particularly the brain, when absorbed through the gut. The translocation of peptidoglycan is an active process regulated by the microbiota-induced parasympathetic pathway through muscarinic acetylcholine receptors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Martin Schwarzer, Umesh Kumar Gautam, Kassem Makki, Anne Lambert, Tomas Brabec, Amelie Joly, Dagmar Srutkova, Pierre Poinsot, Tereza Novotna, Stephanie Geoffroy, Pascal Courtin, Petra Petr Hermanova, Renata C. Matos, Jonathan J. M. Landry, Celine Gerard, Anne-Laure Bulteau, Tomas Hudcovic, Hana Kozakova, Dominik Filipp, Marie -Pierre Chapot-Chartier, Marek Sinkora, Noel Peretti, Ivo Gomperts Boneca, Mathias Chamaillard, Hubert Vidal, Filipe De Vadder, Francois Leulier
Summary: The intestinal microbiota plays a role in postnatal growth, and Lactiplantibacillus plantarum strain LpWJL has been found to support growth in undernourished animals. This strain stimulates insulin-like growth factor-1 (IGF-1) and insulin production and activity. Cell walls from LpWJL, as well as specific molecules, can promote growth despite undernutrition. NOD2 in intestinal epithelial cells is necessary for LpWJL-mediated IGF-1 production and growth promotion in malnourished animals.