Journal
FEBS JOURNAL
Volume 278, Issue 2, Pages 257-272Publisher
WILEY
DOI: 10.1111/j.1742-4658.2010.07943.x
Keywords
coat protein; protein-protein interaction; recombinant movement protein; sobemovirus
Categories
Funding
- Department of Biotechnology
- Department of Science and Technology, New Delhi, India
- Indian Institute of Science, Bangalore, India
- University Grant Commission, New Delhi, India
Ask authors/readers for more resources
Sesbania mosaic virus (SeMV) is a single-stranded positive-sense RNA plant virus belonging to the genus Sobemovirus. The movement protein (MP) encoded by SeMV ORF1 showed no significant sequence similarity with MPs of other genera, but showed 32% identity with the MP of Southern bean mosaic virus within the Sobemovirus genus. With a view to understanding the mechanism of cell-to-cell movement in sobemoviruses, the SeMV MP gene was cloned, over-expressed in Escherichia coli and purified. Interaction of the recombinant MP with the native virus (NV) was investigated by ELISA and pull-down assays. It was observed that SeMV MP interacted with NV in a concentration- and pH-dependent manner. Analysis of N- and C-terminal deletion mutants of the MP showed that SeMV MP interacts with the NV through the N- terminal 49 amino acid segment. Yeast two-hybrid assays confirmed the in vitro observations, and suggested that SeMV might belong to the class of viruses that require MP and NV/coat protein for cell-to-cell movement.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available