Journal
FEBS JOURNAL
Volume 276, Issue 7, Pages 1878-1890Publisher
WILEY
DOI: 10.1111/j.1742-4658.2009.06914.x
Keywords
HCFC1; human; intragenic binding; sterol-regulated; transcriptional regulation
Categories
Funding
- Swedish Research Council for Science and Technology
- Diabetes Association
- Cancer Foundation
- Markus Borgstrom Foundation
- Family Ernfors Fund and Novo Nordisk
- Swedish Research Council
- Novo Nordisk Foundation
- Royal Swedish Academy of Sciences through a grant from the Knut and Alice Wallenberg Foundation
- Swedish Foundation
- Uppsala University
- Swedish University for Agricultural Sciences.
Ask authors/readers for more resources
Sterol regulatory element-binding proteins 1 and 2 (SREBP-1 and SREBP-2) are important regulators of genes involved in cholesterol and fatty acid metabolism, but have also been implicated in the regulation of the cell cycle and have been associated with the pathogenesis of type 2 diabetes, atherosclerosis and obesity, among others. In this study, we aimed to characterize the binding sites of SREBP-1 and RNA polymerase II through chromatin immunoprecipitation and microarray analysis in 1% of the human genome, as defined by the Encyclopaedia of DNA Elements consortium, in a hepatocellular carcinoma cell line (HepG2). Our data identified novel binding sites for SREBP-1 in genes directly or indirectly involved in cholesterol metabolism, e.g. apolipoprotein C-III (APOC3). The most interesting biological findings were the binding sites for SREBP-1 in genes for host cell factor C1 (HCFC1), involved in cell cycle regulation, and for filamin A (FLNA). For RNA polymerase II, we found binding sites at classical promoters, but also in intergenic and intragenic regions. Furthermore, we found evidence of sterol-regulated binding of SREBP-1 and RNA polymerase II to HCFC1 and FLNA. From the results of this work, we infer that SREBP-1 may be involved in processes other than lipid metabolism.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available