Article
Ophthalmology
Li Huang, Limei Sun, Xiaoyu Li, Songshan Li, Ting Zhang, Zhaotian Zhang, Xiaoyan Ding
Summary: Female carriers of NDP mutations exhibited clinical features of familial exudative vitreoretinopathy (FEVR), with 8.3% experiencing decreased visual acuity and 91.7% being asymptomatic. Fundus fluorescein angiography (FFA) revealed peripheral vascular changes in 75.0% of the carriers.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Massimo Zeviani, Valerio Carelli
Summary: The retina is a vulnerable target for defects in oxidative phosphorylation (OXPHOS) due to mitochondrial impairment, resulting in conditions such as retinal dystrophy and optic atrophy. Mutations in mitochondrial DNA (mtDNA) and nuclear genes are implicated in mitochondrial retinopathies, presenting as isolated diseases or part of more complex syndromes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Rocco Latorre, Alan Hegron, Chloe J. Peach, Shavonne Teng, Raquel Tonello, Jeffri S. Retamal, Rafael Klein-Cloud, Diana Bok, Dane D. Jensen, Lena Gottesman-Katz, Jeanette Rientjes, Nicholas A. Veldhuis, Daniel P. Poole, Brian L. Schmidt, Charalabos H. Pothoulakis, Carl Rankin, Ying Xie, Hon Wai Koon, Nigel W. Bunnett
Summary: This study investigated the localization and signaling of protease-activated receptor 2 (PAR(2)) in colitis, revealing that PAR(2) endocytosis sustains protease-evoked inflammation and nociception. PAR(2) in endosomes is a potential therapeutic target for colitis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Etelka Poestyeni, Krisztina Szabadfi, Gyoergy Setalo, Robert Gabriel
Summary: Diabetes and hypertension are complex pathologies with increasing prevalence, often leading to retinal damage. Double neuroprotective treatment was found to be effective for rod bipolar cells and parvalbumin-positive cells in hypertensive diabetic rat retinas.
Article
Multidisciplinary Sciences
Emily H. Stoops, Michael A. Ferrin, Danielle M. Jorgens, David G. Drubin
Summary: This research demonstrates the sequence recruitment of core endocytic proteins and regulatory proteins, as well as the assembly of actin networks in live cells. The study shows that supported lipid bilayers coated with purified yeast WASP recruit downstream endocytic proteins and assemble actin networks. The actin-driven vesicle generation could be a precursor for various vesicle forming processes in different cellular environments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Takahito Moriyama, Fumio Hasegawa, Yoei Miyabe, Kenichi Akiyama, Kazunori Karasawa, Keiko Uchida, Kosaku Nitta
Summary: The study reveals that albumin in podocytes is transported intracellularly through caveolae, entering cells with Fc receptors, moving along actin cytoskeleton, and eventually being degraded or exocytosed.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Ophthalmology
Samantha R. De Silva, Gavin Arno, Anthony G. Robson, Ana Fakin, Nikolas Pontikos, Moin D. Mohamed, Alan C. Bird, Anthony T. Moore, Michel Michaelides, Andrew R. Webster, Omar A. Mahroo
Summary: X-linked retinopathies encompass a variety of progressive and stationary conditions, some with syndromic features and some without. Many diseases are X-linked recessive, but some female carriers also exhibit symptoms, aiding in diagnosis and understanding of disease mechanisms. Some disorders show diverse phenotypes from variants in the same gene, highlighting limitations of current genetic sequencing methods.
PROGRESS IN RETINAL AND EYE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Maria Romanova-Michaelides, Zena Hadjivasiliou, Daniel Aguilar-Hidalgo, Dimitris Basagiannis, Carole Seum, Marine Dubois, Frank Juelicher, Marcos Gonzalez-Gaitan
Summary: Research has found that scaling of the Dpp gradient in the Drosophila wing disc is achieved by increasing the contribution of internalized Dpp molecules to Dpp transport, regulated by the extracellular factor Pentagone. This evolutionary mechanism may act on endocytic trafficking to regulate the range and scaling of the gradient, allowing adaptation of shape and pattern to different sizes of organs in different species.
Article
Biochemistry & Molecular Biology
Arturo Ortin-Martinez, Nicole E. Yan, En Leh Samuel Tsai, Lacrimioara Comanita, Akshay Gurdita, Nobuhiko Tachibana, Zhongda C. Liu, Suying Lu, Parnian Dolati, Neno T. Pokrajac, Ahmed El-Sehemy, Philip E. B. Nickerson, Carol Schuurmans, Rod Bremner, Valerie A. Wallace
Summary: Recent studies have demonstrated that intracellular molecules and organelles can transfer between cells during embryonic development, tissue homeostasis and disease. Through cell transplantation, researchers have observed bidirectional transfer of intracellular material between transplanted and host cells, revealing a process termed material transfer (MT) that requires cell contact and stable microtubule-containing protrusions. Targeting actin remodeling inhibits MT, suggesting a mechanism for horizontal exchange of intracellular material via nanotube-like connections between neurons in vivo.
Article
Biology
Junko Y. Toshima, Ayana Tsukahara, Makoto Nagano, Takuro Tojima, Daria E. Siekhaus, Akihiko Nakano, Jiro Toshima, Sean Munro
Summary: Budding yeast possesses an independent endocytic early/sorting compartment within the trans-Golgi network (TGN) that sorts endocytic cargos. This compartment functions as the target for endocytic vesicles and is involved in recycling to the plasma membrane or transport to the Rab5 endosomal compartment. The Tlg2p protein, a homolog of yeast syntaxin, resides in this compartment and acts as a Q-SNARE for endocytic vesicles.
Article
Biology
Cansu Kuey, Meghane Sittewelle, Gabrielle Larocque, Miguel Hernandez-Gonzalez, Stephen J. Royle
Summary: This study demonstrates that intracellular clathrin-coated vesicle (CCV) formation can be induced using minimal machinery in living cells, and it can occur on various membranes. The study also shows that the formation of these vesicles, called MitoPits, can faithfully recapitulate all stages of CCV formation without the involvement of accessory proteins.
Article
Chemistry, Multidisciplinary
Kwahun Lee, Insub Jung, Teri W. Odom
Summary: This paper discusses how the order of delivery and endosomal distribution affect endosomal pathways of nanoparticles with different surface curvatures. The results show that the sequential incubation of certain nanoconstructs with macrophages can accelerate the formation of late-stage endosomes and result in larger endosomes. The study also demonstrates that the delivery order and endosomal distribution can affect the subcellular trafficking of targeting nanoconstructs. The paper also presents a new approach for nanoscale monitoring of intracellular therapeutics delivery using conventional electron microscopy.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Article
Cell Biology
Lucia-Doina Popov
Summary: This review provides an overview of caveolae-mediated transport across polarized epithelial cell barriers, including the molecular mechanisms, lipid-binding proteins, caveolae architecture, and the intracellular tasks of caveolae during cargo transport. The review also discusses the assembly, detachment, and internalization of caveolae, the regulation of caveolae intracellular routes by signaling molecules, and the transport dysregulation in diseases. Additionally, it highlights the therapeutic possibilities offered by Caveolin-1 expression and caveolae trafficking, as well as the urgent issues to be uncovered.
CELLULAR SIGNALLING
(2022)
Article
Cell Biology
Florian Wilfling, Marko Kaksonen, Jeanne Stachowiak
Summary: Vesicles play a crucial role in cell biology, with intrinsically disordered domains and proteins increasingly recognized for their importance in membrane traffic processes.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Fouad Brahimi, Alba Galan, Sean Jmaeff, Pablo F. Barcelona, Nicolas De Jay, Kurt Dejgaard, Jason C. Young, Claudia L. Kleinman, David Y. Thomas, H. Uri Saragovi
Article
Cell Biology
Maria C. Paz, Pablo F. Barcelona, Paula V. Subirada, Magali E. Ridano, Gustavo A. Chiabrando, Claudia Castro, Maria C. Sanchez
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Ophthalmology
Marilyn A. Marquez, Claudio P. Juarez, Maria C. Sanchez, Jose D. Luna
Summary: This case report describes a unique presentation in a patient with NF1, rubeosis iridis, high IOP, and macular hemorrhages from multiple corkscrew retinal vessels in a well-perfused retina. Treatment with anti-angiogenics led to a good visual outcome.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Multidisciplinary Sciences
Virginia Actis Dato, Maria Cecilia Sanchez, Gustavo Alberto Chiabrando
Summary: IGF-1 regulates GLUT1 translocation and glucose uptake in MGCs through activation of MAPK/ERK and PI3K/Akt pathways, with a molecular association between LRP1 and GLUT1 being significantly reduced by IGF-1. Specific siRNA targeting LRP1 results in decreased GLUT1 expression on the plasma membrane and impaired glucose uptake induced by IGF-1.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Javier Jaldin-Fincati, Serene Moussaoui, Maria Cecilia Gimenez, Cheuk Y. Ho, Charlene E. Lancaster, Roberto Botelho, Fernando Ausar, Roger Brookes, Mauricio Terebiznik
Summary: In this study, we investigated the uptake and trafficking of aluminum oxy-hydroxide (AlOOH) in macrophages and its impact on the trafficking of a pertussis vaccine candidate. Our results demonstrate that AlOOH can be internalized by macrophages and can adsorb Immunoglobulin G, enhancing the capture and uptake of antigen particles. Furthermore, AlOOH traffics to degradative endolysosomal compartments and diverts the vaccine candidate to lysosome-type compartments, potentially playing a crucial role in antigen presentation and T cell activation.
MOLECULAR MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Paula Subirada, Maria C. Paz, Maria V. Vaglienti, Jose D. Luna, Pablo F. Barcelona, Maria C. Sanchez
Summary: Retinopathies are a group of diseases that affect the neurosensory tissue of the eye, leading to neurodegeneration and changes in vascular function and structure. By using animal models and image processing, we can measure retinal vascular parameters such as density, diameter, tortuosity, and neovascular areas. These tools are highly helpful in evaluating and quantifying vascular alterations in retinopathies.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Paula V. Subirada, Maria V. Vaglienti, Mariana B. Joray, Maria C. Paz, Pablo F. Barcelona, Maria C. Sanchez
Summary: This study investigated the changes in autophagy flux during hypoxia and hypoxia-reoxygenation, as well as the effects of Rapamycin and Resveratrol on the gliotic and pro-angiogenic response of Muller glial cells (MGCs) under hypoxic conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Maria Florencia Soler, Rocio del Carmen Bravo-Miana, Dana Maria Negretti-Borga, Paula Subirada, Vanina Alejandra Alamino, Maria Cecilia Sanchez, Ana Carolina Donadio, Claudia Gabriela Pellizas, Maria del Mar Montesinos
Summary: T3-conditioned dendritic cell vaccine shows effective anti-tumor effects in a murine model of colon carcinoma, enhancing immune response by increasing CD86 expression, promoting the production of inflammatory factors, and inducing specific cytotoxic T lymphocytes. T3 also promotes the maturation and activation of human dendritic cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Samaneh Yazdani, Philip J. Bilan, Javier R. Jaldin-Fincati, Janice Pang, Felicia Ceban, Ekambir Saran, John H. Brumell, Spencer A. Freeman, Amira Klip
Summary: This study investigated the transport of glucose in human adipose tissue microvascular endothelial cells. The results showed that glucose was mainly taken up by glucose transporters GLUT1 and GLUT3 and a fraction of glucose was released from the cells. The study also found that a fluorescent glucose derivative entered the cells through endocytosis and was recycled back out.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Victoria L. Tokarz, Rafaela V. S. Pereira, Javier R. Jaldin-Fincati, Sivakami Mylvaganam, Amira Klip
Summary: The lymphatic circulation plays an important role in regulating the transfer of tissue fluid and immune cells. However, obesity can impair the function of the lymphatic vessels. This study found that palmitate, a saturated fatty acid, destabilizes the adherens junctions of lymphatic microvascular endothelial cells (LEC), leading to dysfunction in migration and tube formation. The reorganization of the actin cytoskeleton in LEC may contribute to lymphatic dysfunction in obesity.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Cell Biology
Paula Virginia Subirada, Albana Tovo, Maria Victoria Vaglienti, Jose Domingo Luna Pinto, Horacio Uri Saragovi, Maria Cecilia Sanchez, Agustin Anastasia, Pablo Federico Barcelona
Summary: Choroidal neovascularization (CNV) is a pathological angiogenesis of the choroidal plexus of the retina and is a key feature in the wet form of age-related macular degeneration. Mononuclear phagocytic cells (MPCs) are recruited and activated through p75 neurotrophin receptor (p75(NTR)) in a mouse model of laser-induced CNV, contributing to CNV pathology. Knockout of p75(NTR) or treatment with its antagonist reduces MPC recruitment, glial activation, CNV area, and preserves retinal function in mice with CNV.
Article
Cell Biology
Maria V. Vaglienti, Paula V. Subirada, Mariana B. Joray, Gustavo Bonacci, Maria C. Sanchez
Summary: Inflammation, oxidative stress, and nitrosative stress are involved in the pathogenesis of proliferative retinopathies. Nitro-oleic acid (NO2-OA) shows a protective effect on oxidative damage, gliosis, and the pro-angiogenic response in Muller glial cells.
Review
Neurosciences
Maria C. Sanchez, Gustavo A. Chiabrando
Summary: Muller glial cells (MGCs) play an important role in retinal homeostasis and the development of proliferative retinopathies, while low-density lipoprotein receptor-related protein 1 (LRP1) has been identified as a key player in these processes. Further research in this area could lead to novel therapeutic approaches.
