Article
Cell Biology
Guangyu Ji, Wenjuan Zhou, Jingyi Du, Juan Zhou, Dong Wu, Man Zhao, Liping Yang, Aijun Hao
Summary: The study identified PCGF1 as a crucial epigenetic regulator in sustaining the stem cell-like phenotype of CRC, with increased PCGF1 expression correlated with cancer progression and poor prognosis. Knockdown of PCGF1 inhibited CRC stem cell proliferation and enrichment, ultimately impairing tumor growth in vivo. Mechanistically, PCGF1 activates transcription of CRC stem cell markers by altering histone trimethylation marks on their promoters.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Sarbani Saha, Rumela Bose, Shreeta Chakraborty, Rupasri Ain
Summary: This study reveals the essential role of Cox6B2 in maintaining the stemness of trophoblast stem cells and its regulation by various factors including other genes and microRNAs. These findings have important implications for understanding stem cell biology and placental growth.
Article
Multidisciplinary Sciences
Fanyuan Yu, Feifei Li, Liwei Zheng, Ling Ye
Summary: This study reveals the importance of epigenetic regulation through Ezh2/H3K27me3 in determining the fate of epithelial stem cells (ASCs). It plays a crucial role in maintaining tissue homeostasis and facilitating tissue regeneration.
Editorial Material
Cell Biology
Kaosheng Lv, Wei Tong
Summary: The novel role of Bmi1 in regulating ribosome biogenesis and protein synthesis in hematopoietic stem cells has been discovered. Bmi1 is essential for maintaining HSCs, and its deficiency leads to reduced transplantability and protein stress.
GENES & DEVELOPMENT
(2022)
Article
Cell Biology
Rebecca J. Burgess, Zhiyu Zhao, Daisuke Nakada, Sean J. Morrison
Summary: The study showed that deficiency of Bmi1 leads to increased division of HSCs without inducing senescence. Bmi1 promotes HSC quiescence by negatively regulating ARX expression and promoting proteostasis by suppressing protein synthesis.
GENES & DEVELOPMENT
(2022)
Article
Cell & Tissue Engineering
Yuan Liu, Meimei Huang, Xiaodan Wang, Zinan Liu, Siqi Li, Ye-Guang Chen
Summary: This study reveals the critical role of methyltransferase-like 3 (Mettl3) in maintaining intestinal stem cell (ISC) stemness through N6-methyladenosine (m6A) methylation, independent of cell proliferation. The researchers identified four m6A-modified transcription factors that can restore stemness gene expression in Mettl3-deficient organoids, and also discerned 23 genes unrelated to cell proliferation. These findings demonstrate the importance of m6A modification in sustaining ISC stemness and its uncoupling from cell proliferation.
Article
Multidisciplinary Sciences
Ana Nikolic, Francesca Maule, Anna Bobyn, Katrina Ellestad, Seungil Paik, Sajid A. Marhon, Parinaz Mehdipour, Xueqing Lun, Huey-Miin Chen, Claire Mallard, Alexander J. Hay, Michael J. Johnston, Christopher J. Gafuik, Franz J. Zemp, Yaoqing Shen, Nicoletta Ninkovic, Katalin Osz, Elodie Labit, N. Daniel Berger, Duncan K. Brownsey, John J. Kelly, Jeff Biernaskie, Peter B. Dirks, Darren J. Derksen, Steven J. M. Jones, Donna L. Senger, Jennifer A. Chan, Douglas J. Mahoney, Daniel D. De Carvalho, Marco Gallo
Summary: The histone variant macroH2A2 controls an epigenetic mechanism of self-renewal and suggests new treatment approaches for glioblastoma patients.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Jingjing Hu, Ke Chen, Fangfang Hong, Guosheng Gao, Xiaoyu Dai, Hua Yin
Summary: METTL3 modifies SOCS3 mRNA with m6A to promote the stemness and tumorigenicity of LCSCs.
CANCER GENE THERAPY
(2023)
Article
Medicine, Research & Experimental
Jianxu Chen, Zhijie Xu, Hongbin Huang, Yao Tang, Hong Shan, Fei Xiao
Summary: This study identifies the H3K4 methyltransferase SETD1A and Trithorax group proteins as drivers of cancer stemness in hepatocellular carcinoma (HCC). SETD1A is positively correlated with poor clinical outcome in HCC patients and can activate oncogenes and deactivate tumor suppressor genes through transcriptional activation of histone-modifying enzymes and the activation of oncogenic enhancers and super-enhancers in liver cancer stem cells.
Article
Biochemistry & Molecular Biology
Frank Leon, Parthasarathy Seshacharyulu, Rama K. Nimmakayala, Seema Chugh, Saswati Karmakar, Palanisamy Nallasamy, Raghupathy Vengoji, Satyanarayana Rachagani, Jesse L. Cox, Kavita Mallya, Surinder K. Batra, Moorthy P. Ponnusamy
Summary: Aberrant protein glycosylation plays a significant role in stemness and aggressive metastasis in pancreatic cancer, with the loss of C1GALT1 leading to truncation of CD44 glycosylation and enhanced CSC features. Truncation of O-glycans on CD44 increases NANOG activation, mediating increased CSC activation in PC cells.
Review
Cell Biology
Shanshan Chen, Wenqi Wang, Hor-Yue Tan, Yuanjun Lu, Zhiping Li, Yidi Qu, Ning Wang, Di Wang
Summary: Autophagy is crucial for cellular homeostasis and senescence, with dysregulation implicated in various human diseases. This review highlights the role of autophagy in stem cell maintenance, focusing on its interaction with adult stem cells in disease initiation and progression. Understanding the mechanisms of autophagy in maintaining stemness may provide insights into disease pathogenesis and therapy development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Fengbiao Zhou, Nesrine Aroua, Yi Liu, Christian Rohde, Jingdong Cheng, Anna-Katharina Wirth, Daria Fijalkowska, Stefanie Goellner, Michelle Lotze, Haiyang Yun, Xiaobing Yu, Caroline Pabst, Tim Sauer, Thomas Oellerich, Hubert Serve, Christoph Roellig, Martin Bornhaeuser, Christian Thiede, Claudia Baldus, Michaela Frye, Simon Raffel, Jeroen Krijgsveld, Irmela Jeremias, Roland Beckmann, Andreas Trumpp, Carsten Mueller-Tidow
Summary: The dynamic 2'-O-methylation of ribosomal RNA regulates leukemia stem cell activity in acute myeloid leukemia. The methylation pattern is associated with AML development stage and stem cell gene expression. Enhanced 2'-O-methylation redirects ribosome translation and controls AML stem cell self-renewal.
Review
Cell Biology
Ning Liang, Tao Yang, Qian Huang, Pengfei Yu, Chaoxu Liu, Liusheng Chen, Qian Wang, Gang Wang, Xianli He
Summary: Primary liver cancer consists of hepatocellular carcinoma, cholangiocarcinoma, hepatoblastoma, and combined hepatocellular carcinoma and cholangiocarcinoma. Recent studies have found differences in cancer stem cell properties among different liver cancer types. Liver cancer stem cells, also known as liver tumor-initiating cells, are considered to be drivers of tumor initiation and metastasis, and are regulated by various mechanisms and factors. This review presents an analysis of cancer stemness in different liver cancer types, evaluates the mechanisms of cancer stemness maintenance in liver cancer stem cells, and discusses potential treatments for eradicating liver cancer stem cells.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Swarnabh Bhattacharya, Abhishek Mukherjee, Sabrina Pisano, Shalini Dimri, Eman Knaane, Anna Altshuler, Waseem Nasser, Sunanda Dey, Lidan Shi, Ido Mizrahi, Noam Blum, Ophir Jokel, Aya Amitai-Lange, Anna Kaganovsky, Michael Mimouni, Sergiu Socea, Mohamad Midlij, Beatrice Tiosano, Peleg Hasson, Chloe Feral, Haguy Wolfenson, Ruby Shalom-Feuerstein
Summary: The fate decisions of stem cells depend on signals from their microenvironment. By studying corneal epithelial stem cells, it was found that the unique biomechanical property of the niche supports the nuclear localization and function of Yes-associated protein (YAP). These findings suggest that stem cells can sense biomechanical niche signals, and manipulating the mechanosensory machinery could have implications for stem cell expansion in regenerative therapy.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Karolin Walter, Eva Rodriguez-Aznar, Monica S. Ventura Ferreira, Pierre-Olivier Frappart, Tabea Dittrich, Kanishka Tiwary, Sabine Meessen, Laura Lerma, Nora Daiss, Lucas-Alexander Schulte, Zeynab Najafova, Frank Arnold, Valentyn Usachov, Ninel Azoitei, Mert Erkan, Andre Lechel, Tim H. Brummendorf, Thomas Seufferlein, Alexander Kleger, Enrique Tabares, Cagatay Gunes, Steven A. Johnsen, Fabian Beier, Bruno Sainz Jr, Patrick C. Hermann
Summary: This study demonstrates the critical role of telomerase regulation in maintaining stemness in pancreatic CSCs and examines the effects of telomerase inhibition as a potential treatment option for pancreatic cancer. This may significantly enhance our understanding of PDAC tumor biology and potentially lead to improved treatment for pancreatic cancer patients.