Review
Physiology
Jun Zhang, Huikai Yuan, Xiaoqiang Yao, Shuo Chen
Summary: Mammalian expression systems, particularly HEK-293 cells, combined with electrophysiological studies, have greatly improved our understanding of ion channels. It was previously assumed that endogenous ion channels in HEK-293 cells were negligible, but recent studies have shown their presence should be considered. This article summarizes the different endogenously expressed ion channels in HEK-293 cells and highlights the variability in expression patterns and channel profiles. A better understanding of endogenous ion channels can help minimize potential issues in characterizing heterologously expressed ion channels. It is recommended to examine the functional profile of HEK-293 cells before transfection, especially when endogenous ion channels are not overwhelmed by exogenous ion channels.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Antonela Fuselli, Maria de Los Milagros Burgi, Ricardo Kratje, Claudio Prieto
Summary: In this study, the soluble domain of hSCF was expressed in HEK293 cells, resulting in highly purified rhSCF with increased proliferative activity compared to bacteria-derived hSCF. Three monoclonal antibodies (mAbs) with high specificity for the glycoprotein were obtained using hybridoma technology. These antibodies were able to detect bacteria-derived and glycosylated rhSCF, and were suitable for quantitative analysis and immunodetection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Simona Vaitkiene, Laura Bekere, Gunars Duburs, Rimantas Daugelavicius
Summary: The study evaluated the interaction of synthesized compound CSDP+ and fluconazole with yeast and mammalian cells. The combination was found to be non-toxic to mammalian cells and reduced C. albicans adhesion but led to overexpression of efflux-related resistance genes in the fungus. Measurements of efflux pumps and gene expression showed that mammalian cells were not affected by CSDP+ concentrations affecting C. albicans.
Article
Biochemical Research Methods
Sang Yoon Lee, Minhye Baek, Gyun Min Lee
Summary: HEK293 cells with glycosylation machinery are a viable option for stable expression of therapeutic glycoproteins. DHFR/MTX-mediated gene amplification in these cells can lead to increased productivity of monoclonal antibodies (mAbs), with some high producing clones maintaining this productivity during long-term culture despite a general decrease in productivity among clones over time.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Physiology
David H. Vandorpe, John F. Heneghan, Joshua S. Waitzman, Gizelle M. McCarthy, Angelo Blasio, Jose M. Magraner, Olivia G. Donovan, Lena B. Schaller, Shrijal S. Shah, Balajikarthick Subramanian, Cristian V. Riella, David J. Friedman, Martin R. Pollak, Seth L. Alper
Summary: Two missense variants of APOL1 can attenuate the severity of Trypanosoma brucei infection but increase the risk of kidney disease in Americans of African descent. The ion channel activity of APOL1 has been characterized in HEK-293 cells, and similar activity has been detected in human glomerular podocytes. This suggests that APOL1-mediated channel activity could be a therapeutic target for treating APOL1-associated kidney diseases.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Raphaela Mello Zamudio, Jaine Gonsalves Garcia, Camila Mosca Barboza, Adriana Candido Rodrigues, Tamires Santos de Arruda, Ana Lee Aparecida Francisco, Willian de Oliveira Fahl, Juliana Galera Castilho, Maria Cristina Carlan da Silva, Helena Beatriz de Carvalho Ruthner Batista
Summary: Our study evaluated the susceptibility of the HEK-293 cell line to RTCIT compared to Neuro-2a, finding a higher positive rate in HEK-293 with the adsorption protocol and 72 h incubation period. Despite slightly higher sensitivity of Neuro-2a, HEK-293 cells offer advantages of faster results and easier growth, making them a potential alternative for RTCIT.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Mehtap Kara, Tugce Boran, Ezgi Oztas, Ayse Tarbin Jannuzzi, Sibel Ozden, Gul Ozhan
Summary: Zoledronic acid induces nephrotoxicity in HEK-293 cells through increased oxidative stress and ER stress leading to apoptosis.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Biology
Chun Chen, Jai-Sing Yang, Chi-Cheng Lu, Yu-Tse Wu, Fu-An Chen
Summary: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in sports medicine, but can induce acute renal damage. Quercetin protects against indomethacin-induced HEK293 cell apoptosis by inhibiting apoptotic signals and regulating mitochondrial membrane potential, reducing ROS production.
Article
Virology
Sachi Horibata, Tadahisa Teramoto, Navin Vijayarangan, Skyler Kuhn, Raji Padmanabhan, Sona Vasudevan, Michael Gottesman, Radhakrishnan Padmanabhan
Summary: The HEK-293 cell line, created in 1977 through transformation with adenovirus type 5 DNA, was found to support Zika virus replication. RNA-seq analysis revealed differential transcription of 659 genes in Zika-infected HEK-293 cells, with the top 10 genes upregulated being involved in antiviral and inflammatory responses.
Article
Neurosciences
Miriam Kessi, Baiyu Chen, Langui Pan, Li Yang, Lifen Yang, Jing Peng, Fang He, Fei Yin
Summary: Investigations showed that the p.E411D, p.V622G, and p.A272V mutations in the CACNA1C gene decrease the expression levels of CACNA1C proteins and impair mitochondrial and lysosomal functions, which may contribute to the pathogenesis of CACNA1C-related disorders.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Avik Banerjee, Jiachen Huang, Scott A. Rush, Jackelyn Murray, Aaron D. Gingerich, Fredejah Royer, Ching-Lin Hsieh, Ralph A. Tripp, Jason S. McLellan, Jarrod J. Mousa
Summary: This study identified immunodominant antigenic epitopes on the hMPV F protein and identified a monoclonal antibody (mAb) therapy for hMPV F disease prevention and treatment. Additionally, a prefusion-specific epitope on the hMPV F protein was discovered.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Chun yan Guo, Zhan kui Jin, Qing Feng, Yang meng Feng, Li jun Sun, Cui xiang Xu, Yu lian Zhang
Summary: This study examined the relationship between heterophilic epitopes on H5N1 hemagglutinin (HA) and disease. The results showed that these epitopes can induce the production of antibodies that cross-react with kidney tissue, providing materials for understanding the mechanism of renal diseases caused by influenza virus infection.
MICROBIAL PATHOGENESIS
(2023)
Article
Materials Science, Multidisciplinary
Gulam Abbas, Kijay Bahadur Singh, Narinder Kumar, Anamika Shukla, Devesh Kumar, Gajanan Pandey
Summary: The study successfully synthesized PEG-coated α-Fe2O3 NPs and demonstrated their potential applications in combating renal carcinoma cell lines. The NPs not only showed anti-cancer activity, but also exhibited excellent biocatalytic activity and antioxidant capability.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Tomoki Sakamoto, Yudai Yamaoki, Takashi Nagata, Masato Katahira
Summary: Researchers successfully introduced oligodeoxynucleotides that can form triplex structures in vitro into living human cells and observed the characteristic signals of triplex structures through in-cell NMR spectra, providing the first evidence of the formation of parallel and antiparallel triplex structures in living human cells.
CHEMICAL COMMUNICATIONS
(2021)
Article
Cell Biology
Meng Yuan, Ziqi Feng, Huibin Lv, Natalie So, Ivana R. Shen, Timothy J. C. Tan, Qi Wen Teo, Wenhao O. Ouyang, Logan Talmage, Ian A. Wilson, Nicholas C. Wu
Summary: This study reveals that the ability of the human immune system to generate antibodies can be influenced by immunoglobulin V-gene allelic polymorphisms. Through the analysis of antibody-antigen structures, it is found that V-gene allelic polymorphisms in the antibody paratopes determine the antibody binding activity. Additionally, minor V-gene allelic polymorphisms also play an important role in the generation of broadly neutralizing antibodies.