Article
Engineering, Biomedical
Kevin Collon, Jennifer A. Bell, Stephanie W. Chang, Matthew C. Gallo, Osamu Sugiyama, Carolyn Marks, Jay R. Lieberman
Summary: This study compared different cell seeding techniques in transduced cell-based gene therapy. Static and negative pressure seeding techniques showed the highest seeding efficiency, while orbital shaking resulted in the greatest increase in BMP-2 production per cell. Low density cell suspensions were associated with the highest seeding efficiency, cell distribution uniformity, and the greatest increase in BMP-2 production after seeding.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Sandra Stamnitz, Agnieszka Krawczenko, Urszula Szalaj, Zaneta Gorecka, Agnieszka Antonczyk, Zdzislaw Kielbowicz, Wojciech Swieszkowski, Witold Lojkowski, Aleksandra Klimczak
Summary: FGF-2 and BMP-2 enhance the osteogenic differentiation potential of MSCs grown on a scaffold, making them promising tools for bone regeneration. This was confirmed through in vitro and in vivo experiments.
Review
Engineering, Biomedical
Yiting Lou, Huiming Wang, Guanchen Ye, Yongzheng Li, Chao Liu, Mengfei Yu, Binbin Ying
Summary: This review discusses the important role of periosteum in bone defect repair and regeneration, as well as the need for developing tissue-engineered periosteum (TEP). The construction strategies for TEP are explored from the perspectives of materials, mechanical properties, cell-based strategies, and combination with growth factors. Current challenges and future perspectives for TEP development are also discussed.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Shuqin Zhang, Denghui Xie, Qun Zhang
Summary: This review summarizes the current status and prospects of bone regeneration therapy, introducing the roles of scaffolds and stem cells in tissue engineering, common treatment strategies for bone metabolism-related diseases, and the direction for further development.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Mian Wu, Lifeng Chen, Yuhan Qi, Hai Ci, Shan Mou, Jie Yang, Qiaoyu Yuan, Weiqi Yao, Zhenxing Wang, Jiaming Sun
Summary: Using umbilical cord mesenchymal stem cells (hUCMSCs) as seed cells for tissue-engineered breast construction has shown significant advantages, promoting adipose tissue regeneration and enhancing angiogenesis. The transplantation of hUCMSCs was found to increase fat tissue content, reduce macrophage numbers, and exhibit good differentiation capability in adipose tissue.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Engineering, Biomedical
Jinsong Wang, Guobao Chen, Zhong M. Chen, Fu P. Wang, Bin Xia
Summary: The role of periosteum in the treatment of bone defects has been gradually discovered, but there are still challenges due to limited transplantable periosteum. Biomimetic periosteal scaffolds that mimic the composition and structure of natural periosteum have emerged. This article reviews the current preparation methods of biomimetic periosteal scaffolds based on various biomaterials, providing a systematic perspective for future preparation.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Biophysics
Peng Liu, Tiecheng Qiu, Jiabing Liu, Xiaoxi Long, Xianwei Wang, Hemin Nie, Mengqiang Yu, Chao Ma, Nan Lin, Swee Hin Teoh, Zuyong Wang
Summary: Researchers have developed a biomimetic film with the potential to be used as a synthetic periosteum. This film, made of a blend of poly(epsilon-caprolactone) and microtantalum particles, shows enhanced surface wettability and mechanical strength. It also promotes cell proliferation, alignment, and osteogenic enhancement.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Cell & Tissue Engineering
Che-Chang Tu, Nai-Chen Cheng, Jiashing Yu, Yi-Xuan Pan, Wei-Chiu Tai, Yin-Chuan Chen, Po-Chun Chang
Summary: This study evaluates the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel (mTG) for treating diabetic periodontal wounds and craniofacial defects. The results show that osteogenically pretreated ADsp-mTG can promote the regeneration of diabetic osseous defects. This suggests that ADsp-mTG can facilitate diabetic periodontal wound healing and craniofacial osseous defect regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Medicine, General & Internal
Hideaki Kagami, Minoru Inoue, Hideki Agata, Izumi Asahina, Tokiko Nagamura-Inoue, Masataka Taguri, Arinobu Tojo
Summary: By optimizing the cell-processing protocol, stable bone regeneration can be achieved with comparable quality and less fluctuation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Sumeyra Nur Fuerkaiti, Anil Sera Cakmak, Cagatay Karaaslan, Menemse Gumusderelioglu
Summary: This study demonstrated that silencing Noggin using siRNA induces osteogenic differentiation in reduced doses of BMP-2 for scaffold-based bone regeneration. The siNoggin-transfected cells showed enhanced osteogenic effects and mineralization, suggesting a potential safe therapeutic approach for tissue regeneration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Polymer Science
Hadis Gharacheh, Murat Guvendiren
Summary: Composite hydrogel bioink, formulated by incorporating human allograft bone particles in a methacrylated alginate formulation, enhances osteogenesis of adult human mesenchymal stem cells (hMSCs) for bone tissue engineering. The bioink is suitable for extrusion-based 3D bioprinting, and in vitro studies show significantly enhanced osteogenic differentiation of hMSCs cultured within the bioprinted composite scaffolds.
Article
Biophysics
Jisun Park, Su Jeong Lee, Tae Gon Jung, Jun Hee Lee, Wan Doo Kim, Jae Young Lee, Su A. Park
Summary: Three-dimensional (3D) bioprinting allows for precise fabrication of scaffolds for tissue engineering, particularly those supporting cell attachment and osteogenic differentiation essential for bone tissue regeneration. Coating PCL scaffolds with PDA and depositing HA nanoparticles, followed by BMP-2 immobilization, promotes osteoblast proliferation and differentiation for potential bone tissue engineering applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Surgery
Safi Ali-Khan, Christopher Runyan, Gil Nardini, Pradip Shetye, David Staffenberg, Joseph G. McCarthy, Roberto L. Flores
ANNALS OF PLASTIC SURGERY
(2018)
Article
Otorhinolaryngology
Meade C. Edmunds, T. Alex McKnight, Christopher M. Runyan, Brian W. Downs, Jordan L. Wallin
JAMA OTOLARYNGOLOGY-HEAD & NECK SURGERY
(2019)
Article
Surgery
Nicholas Walker, Veronica M. Jones, Lauren Kratky, Haiying Chen, Christopher M. Runyan
ANNALS OF PLASTIC SURGERY
(2019)
Letter
Dentistry, Oral Surgery & Medicine
Elcin Esenlik, Travis Gibson, Serena Kassam, Yuki Sato, Judah Garfinkle, Alvaro A. Figueroa, Fawzi AlQatami, Christopher Runyan, Michael Alperovich, Michael S. Golinko, Catherine Lee, Athina Chatzigianni, Anastasios A. Zafeiriadis, Pedro Santiago, Banafsheh Hosseinian, Emine Ulug Kaygisiz, Neslihan Ucuncu, Belma Isik Aslan, Fatma Deniz Uzuner, Ayse Gulsen, Atilim Akkurt, Seher Gunduz Aslan, Mariana Sabas, Maria Ana Munoz-Mendoza, Daisy Masis, Lizbeth Holguin, Aracely Granados, Nancy Edith Rojas, Beatrice Campo, Kamile Keskin, M. Okan Akcam, Kristen M. Lowe, Paolo G. Morselli, Lucia Pannuto, Ignacio Nacho Yarza, Ana Tejero Martinez, Esra Yuksel Coskun, Sagit Nissan
CLEFT PALATE-CRANIOFACIAL JOURNAL
(2020)
Article
Ophthalmology
Shamit S. Prabhu, Scotty A. Chung, Philip J. Brown, Christopher M. Runyan
Summary: The study tested a novel technique using custom-printed midface contour models and orbital floor stamps to guide reconstruction of orbital floor blowout fractures. The cost of production was $131 and the average time for the process was 170 minutes. Patients experienced resolution of symptoms and improvements in enophthalmos with the use of these patient-specific custom-contoured orbital floor stamps. Further studies are needed to assess long-term outcomes.
OPHTHALMIC PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Surgery
Christopher M. Runyan, Jungwon G. Park, Lauren Blaha, Kyle S. Gabrick, Robert K. Townsend, Elizabeth Owens, Daniel E. Couture, Lisa R. David
JOURNAL OF CRANIOFACIAL SURGERY
(2020)
Article
Surgery
Christopher M. Runyan, Kyle S. Gabrick, Jungwon G. Park, Dominic Massary, Kshipra Hemal, Elizabeth S. Owens, James T. Thompson, Daniel Couture, Lisa R. David
PLASTIC AND RECONSTRUCTIVE SURGERY
(2020)
Article
Dentistry, Oral Surgery & Medicine
Elliot Le, Peter Shrader, Hayden Bosworth, Jillian Hurst, Benjamin Goldstein, Amelia Drake, Jeyhan Wood, Lisa R. David, Christopher M. Runyan, Joao Ricardo Nickenig Vissoci, Matthew Harker, Alexander C. Allori
CLEFT PALATE-CRANIOFACIAL JOURNAL
(2020)
Article
Surgery
Ludmila Chandler, Omar Allam, Kitae E. Park, John Smetona, Kyle S. Gabrick, Robin T. Wu, Chelsea Morgan, Sydney E. Park, Leah A. Chapman, Daniel E. Couture, Lisa R. David, Christopher M. Runyan, John A. Persing, Michael Alperovich
JOURNAL OF CRANIOFACIAL SURGERY
(2020)
Article
Surgery
Veronica Morgan Jones, Sydney Gillian Thomas, Robert Siska, Dominic Massary, Lisa Renee David, Claire Sanger Dillingham, Anatolie Crihan, Silvia Railean, Christopher M. Runyan
Summary: This study reviewed the experience of spring-assisted surgery (SAS) in treating 13 patients with sagittal craniosynostosis in Moldova from 2011 to 2018. The average age at surgery was 4.0 months, with a mean surgery length of 62.7 minutes and average post-operative recovery time of 30.9 hours. No complications requiring surgical revision were reported, and all patients experienced subjective improvement in scaphocephaly after treatment.
JOURNAL OF CRANIOFACIAL SURGERY
(2021)
Article
Surgery
Shamit S. Prabhu, Kshipra Hemal, Christopher M. Runyan
Summary: A study compared patient presentation, surgical decision-making, and outcomes between isolated orbital floor fractures (I-OF) and zygomaticomaxillary complex fractures (Z-OF). Differences were found in symptoms presentation, preoperative observational periods, postoperative motility restrictions, and risk for eyelid complications between the two fracture types. Z-OF fractures had reduced postoperative motility defects but higher risk of eyelid complications compared to I-OF fractures.
JOURNAL OF CRANIOFACIAL SURGERY
(2021)
Article
Surgery
Nicholas J. Walker, Jungwon G. Park, Jacob C. Maus, Vida Motamedi, Ryan E. Rebowe, Christopher M. Runyan, Scott L. Tucker
Summary: This study compared complication rates between different BMI groups in subpectoral and prepectoral implant-based breast reconstruction. It found that patients with a BMI greater than 35 kg/m² had higher rates of implant exposure in the prepectoral group, and patients with a BMI greater than 25 kg/m² had more minor asymmetry in prepectoral reconstruction. Regardless of technique, the odds of reoperation increased with each point increase in BMI.
ANNALS OF PLASTIC SURGERY
(2021)
Article
Surgery
Michael Alperovich, Christopher M. Runyan, Kyle S. Gabrick, Robin T. Wu, Chelsea Morgan, Sydney E. Park, Leah A. Chapman, Daniel E. Couture, Lisa R. David, John A. Persing
Summary: A comparison study of long-term neurocognitive outcomes in patients with sagittal synostosis who underwent spring-assisted surgery or cranial vault remodeling showed that the latter group had higher intelligence quotient and visuomotor integration scores. However, there were no significant differences in academic achievement between the two groups. Further research is needed to identify factors contributing to cognitive outcome disparities.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Orthopedics
Suman Medda, Matthew A. King, Christopher M. Runyan, John Frino
Summary: The video reviews the technique of using vascularized fibula flap for pediatric tibia reconstruction. A 4-year-old boy with tibial nonunion and bone loss was successfully treated with this method and achieved independent ambulation.
JOURNAL OF ORTHOPAEDIC TRAUMA
(2021)
Article
Surgery
Lyfong S. Lor, Dominic A. Massary, Scotty A. Chung, Philip J. Brown, Christopher M. Runyan
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2020)