Journal
EXTREMOPHILES
Volume 16, Issue 2, Pages 355-361Publisher
SPRINGER TOKYO
DOI: 10.1007/s00792-011-0418-8
Keywords
High-pressure; Prokaryote; Halophile; Adaptation; Osmotic stress; Oxidative stress; Membrane composition
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Funding
- W.M. Keck Foundation [2007-6-29]
- International Balzan Prize Foundation
- Carnegie/DOE Alliance Center (CDAC)
- NASA Astrobiology Institute [NNA09DA81A]
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In this study, we examined the high-pressure survival of a range of prokaryotes not found in high-pressure environments to determine the effects of adaptations to osmotic and oxidative stresses on piezo-resistance. The pressure survivals of Halobacterium salinarum NRC-1, Deinococcus radiodurans R1, and Chromohalobacter salexigens were compared to that of Escherichia coli MG1655. C. salexigens, which uses the compatible solute ectoine as an osmolyte, was as piezo-sensitive as E. coli MG1655, suggesting that ectoine is not a piezolyte. D. radiodurans R1 and H. salinarum NRC-1, both resistant to oxidative stress, were found to be highly piezo-resistant. H. salinarum NRC-1 showed nearly full survival after pressurization up to 400 MPa; a survival 3.5 log units higher than E. coli MG1655. This piezo-resistance was maintained in H. salinarum NRC-1 for pressurizations up to 1 h. We hypothesize that the high-pressure resistance of H. salinarum NRC-1 is due to a combination of factors including cell envelope structure and the presence of intracellular salts.
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