Review
Biochemistry & Molecular Biology
Irene Messana, Barbara Manconi, Tiziana Cabras, Mozhgan Boroumand, Maria Teresa Sanna, Federica Iavarone, Alessandra Olianas, Claudia Desiderio, Diana Valeria Rossetti, Federica Vincenzoni, Cristina Contini, Giulia Guadalupi, Antonella Fiorita, Gavino Faa, Massimo Castagnola
Summary: In this review, the main post-translational modifications leading to multiple proteoforms in the human salivary proteome are extensively described, along with their potential role. The majority of the data reported in this review were obtained through over twenty-five years of research on human saliva, primarily using a top-down strategy. Various proteolytic cleavage products, salivary protein families, phospho-proteome, sulfo-proteomes, glycation, enzymatic glycosylation, citrullination, N- and C-terminal post-translational modifications, and miscellaneous other modifications are discussed in different sections of the manuscript. Furthermore, the impact and relevance of these findings in the development of new diagnostic biomarkers for clinical practice are emphasized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Jessica M. Lewis, Nichollas E. Scott
Summary: CRISPRi was used to investigate the effects of silencing O-linked glycosylation in four species of Burkholderia. The results showed that inducible silencing of PglL did not abolish glycosylation completely and did not lead to phenotypic changes or alterations in proteome associated with glycosylation null strains. In addition, high levels of rhamnose induction of CRISPRi had extensive impacts on the Burkholderia proteomes, making it difficult to determine the specific effects driven by CRISPRi guides without appropriate controls.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Juliane Hermann, Leon Schurgers, Vera Jankowski
Summary: Post-translational modifications play a key role in protein function and disease progression, but their reliable detection and quantification remain a challenge in clinical diagnosis. Mass spectrometry offers a solution, but its clinical translation still faces challenges.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Ivana Samarzija
Summary: The specific functional form of a protein is mostly achieved through dynamic interactions of many enzymes in post-translational modifications, which cells widely use to respond to stimuli, regulate transcription, and maintain proteostasis. Post-translational modifications play important roles in driving prostate cancer, particularly in regulating protein activity at the level of post-translational modifications.
Review
Immunology
You-Wei Wang, Jia-Chen Zuo, Chong Chen, Xiao-Hong Li
Summary: Post-translational modifications (PTMs) covalently attach functional groups to proteins, leading to structural and functional changes. PTMs play a significant role in regulating liver cancer development and the immune response against cancer. Immunotherapies that harness the immune system's potential have shown promise in improving cancer patient prognosis and survival. This review provides a comprehensive summary of key PTMs and their potential targets to enhance immunotherapies against liver cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Sining Chen, Dandan Li, Zhipeng Zeng, Wei Zhang, Hongliang Xie, Jianming Tang, Shengyou Liao, Wanxia Cai, Fanna Liu, Donge Tang, Yong Dai
Summary: The study investigated the impact of Khib on the progression of OACC by influencing the enzyme activity of the glycolysis pathway. The findings provide new therapeutic options for OACC.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Julia Kraxner, Charlotta Lorenz, Julia Menzel, Iwan Parfentev, Ivan Silbern, Manuela Denz, Henning Urlaub, Blanche Schwappach, Sarah Koester
Summary: The mechanical properties of biological cells are influenced by the cytoskeleton, with phosphorylation serving as a fast mechanism for mechanical modulation. Phosphorylation can soften filaments, and the binding of 14-3-3 protein to phosphorylated filaments further enhances this effect, potentially preserving the softening and altering cell mechanics in the cell.
Review
Biochemistry & Molecular Biology
Yanmei Chen, Yi Wang, Jun Yang, Wenbin Zhou, Shaojun Dai
Summary: This paper reviews recent breakthroughs and strategies in unraveling the diversity of the plant proteome, focusing on the methods used to analyze posttranslational modifications (PTMs), protein localization, and the organization of proteins into functional modules. It also discusses PTM crosstalk, multiple PTMs temporally regulating protein life cycles, quantitative studies using MS to measure protein turnover rates, and future directions in the study of the plant proteome.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Filippo Mori, Letizia Natali, Romano Danesi, Silvia Nannizzi, Claudio Farina
Summary: The study examined seven commercially available HSA preparations for clinical use and found differences in PTMs among different commercial albumins. Additionally, an increase in HSA-DA isoform and new oxidative modifications were observed in these samples at the end of the stability time.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Jiena Liu, Qin Wang, Yujuan Kang, Shouping Xu, Da Pang
Summary: Breast cancer is the most common malignant tumor in women worldwide, and post-translational modifications (PTMs) of proteins play important roles in tumorigenesis and progression. However, the study of non-histone modifications, particularly those other than phosphorylation, has been relatively limited. This review aims to explore the functions and impact of these PTMs in breast cancer, and emphasizes the importance of developing novel therapeutic drugs targeting PTMs and biological markers for breast cancer detection.
CELL AND BIOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Crisalejandra Rivera-Perez, Norma Y. Hernandez-Saavedra
Summary: This review discusses the common post-translational modifications of shell matrix proteins (SMPs) and their role in shell biomineralization, as well as recent studies on recombinant production of SMPs. It shows that PTMs are widely distributed in SMPs and may modulate their activity in crystal growth formation and differentiation.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
M. Plouviez, M. Abyadeh, M. Mirzaei, M. Hasan, J. A. Paulo, B. Guieysse
Summary: Algae can efficiently harvest and store phosphorus as intracellular polyphosphates. Understanding the proteomic response of Chlamydomonas reinhardtii under different phosphorus availability conditions is essential for engineering phosphorus removal and recovery. The study found that Chlamydomonas reinhardtii increases proteins involved in phosphorus assimilation and storage under phosphorus depletion, while increases proteins involved in ribosome structure and synthesis under phosphorus repletion.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Review
Biochemistry & Molecular Biology
Erin Q. Jennings, Kristofer S. Fritz, James J. Galligan
Summary: This review highlights the biochemical genesis and regulation of post-translational modifications (PTMs), which alter protein structure, function, and localization, and play a pivotal role in physiological and pathophysiological conditions.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Immunology
Neil G. Rumachik, Stacy A. Malaker, Nicole K. Paulk
Summary: Advances in gene therapy product and process development have improved our understanding of the basic biology of recombinant AAV, with a particular focus on post-translational modifications of the capsid protein. Concerns about immunogenic responses to high doses of systemically administered rAAV have emphasized the importance of cataloging and tracking potentially immunogenic components of vector lots.
FRONTIERS IN IMMUNOLOGY
(2021)
