Article
Endocrinology & Metabolism
Hicham Benabdelkamel, Mohamed Rafiullah, Afshan Masood, Abdulaziz Alsaif, Mohthash Musambil, Assim A. Alfadda
Summary: This study used proteomics approach to analyze the alterations in the thyroid tissue proteome in patients with benign diffuse goiter. The results showed significant changes in the expression of tissue proteins, particularly in signaling pathways and metabolic pathways.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biology
Muhammad Mujammami, Mohamed Rafiullah, Assim A. Alfadda, Khalid Akkour, Ibrahim O. Alanazi, Afshan Masood, Mohthash Musambil, Hani Alhalal, Maria Arafah, Anas M. Abdel Rahman, Hicham Benabdelkamel
Summary: This study investigated the role of diabetes in endometrial cancer using a proteomic approach and identified differentially expressed proteins that may be related to cancer metastasis.
Article
Chemistry, Analytical
Aljoscha Korber, Joel D. Keelor, Britt S. R. Claes, Ron M. A. Heeren, Ian G. M. Anthony
Summary: In this study, a new method called fast mass microscopy is proposed, which combines a Timepix3 detector with continuously sampling secondary ion mass spectrometry mass microscope to achieve high throughput imaging at lower mass resolution compared to conventional MSI. This method enables the acquisition of submicron, gigapixel images of fingerprints and rat tissue at significantly faster speeds than traditional microprobe-mode MSI.
ANALYTICAL CHEMISTRY
(2022)
Article
Reproductive Biology
Giusy Rusco, Mariola Slowinska, Michele Di Iorio, Silvia Cerolini, Angela B. Maffione, Andrzej Ciereszko, Nicolaia Iaffaldano
Summary: This study compared the whole proteome of fresh and cryopreserved rabbit semen and found that cryopreservation led to changes in protein composition. These changes were associated with energy metabolism, protein quality control, reproductive processes, and cell death/survival regulation, resulting in a significant decrease in the motility, viability, and fertilizing ability of post-thawing rabbit sperm.
Article
Chemistry, Analytical
Jia Yi, Yueqing Shen, Yi Yang, Chengpin Shen, Baohong Liu, Liang Qiao, Yan Wang
Summary: By characterizing gingival crevicular fluid (GCF) sediments and identifying potential protein biomarkers using mass spectrometry techniques, this study has contributed to the early diagnosis of periodontitis and understanding of its pathogenesis. The identified biomarkers hold potential value for accurate monitoring and can be utilized by non-specialists for widespread screening.
Article
Food Science & Technology
Xue Li, Xiaorong Deng, Xin Guo, Yabo Wei, Yunfeng Zhao, Xiaobing Guo, Xinrong Zhu, Jian Zhang, Ling Hu
Summary: The present study investigates the freshness indicator protein of Coregonus peled and the effects of oxidation on the quality of fish during 4 degrees C storage. A total of 382 differential proteins were identified, and 20 proteins related to oxidation were found to have the potential as biomarkers for protein oxidative damage. Actin was identified as an indicator for fish softening during postmortem storage. The changes in protein structure and biomarkers were influenced by oxidation. Bioinformatics analysis suggested that differentially expressed proteins participated in the metabolic pathway during 4 degrees C storage.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Plant Sciences
Meenakshi Arya, Satya Prakash, Yaiphabi Sougrakpam, Renu Deswal
Summary: The study identified leaf cuticle proteins from Brassica juncea using organic solvents, analyzing them through gel-based and quantitative shotgun proteomics. Most of the proteins had signal peptides supporting extracellular localization and were predicted to be involved in various activities such as defense response, fatty acid binding, and nutrient reservoir activity. The study also explored the roles of specific proteins like Myrosinase and GDSL esterase/lipase ESM1 in cuticle structuring and defense, as well as the glucosinolate-myrosinase system, PTM crosstalk, and antifreeze activity as stress adaptive strategies in B. juncea.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Prasad M. Govindaiah, Naveena B. Maheswarappa, Rituparna Banerjee, Bidyut Prava Mishra, Balaji B. Manohar, Sowmya Dasoju
Summary: This study evaluated the impact of different slaughter methods on meat quality and various biochemical and hormonal parameters. The results showed that electrical stunning had some effect on meat quality, while there were no significant differences in blood parameters between the non-stunning and stunning groups. Proteomic analysis identified differentially abundant proteins between the two groups.
Article
Biochemistry & Molecular Biology
Eliza Matuszewska, Szymon Plewa, Dagmara Pietkiewicz, Kacper Kossakowski, Joanna Matysiak, Grzegorz Rosinski, Jan Matysiak
Summary: Bee pollen is a valuable medicinal and nutritional product, but its consumption may cause adverse effects due to its unknown composition. This study identified bioactive proteins, including potential allergens, in bee pollen collected in Poland. Standardizing bee pollen is crucial due to its content of both beneficial and harmful compounds, and awareness of allergens can prevent health risks associated with consuming these popular superfoods.
Article
Oncology
Devalingam Mahalingam, Leonidas Chelis, Imran Nizamuddin, Sunyoung S. Lee, Stylianos Kakolyris, Glenn Halff, Ken Washburn, Kristopher Attwood, Ibnshamsah Fahad, Julia Grigorieva, Senait Asmellash, Krista Meyer, Carlos Oliveira, Heinrich Roder, Joanna Roder, Renuka Iyer
Summary: This study utilizes a machine learning algorithm along with mass spectral data and AFP values from serum samples to detect HCC with high sensitivity and specificity. The results suggest that this method can improve cancer detection, especially for small lesions.
Article
Food Science & Technology
Katrien Dierickx, Samantha Presslee, Virginia L. Harvey
Summary: This research presents an alternative proteomic technique for reliable species identification of fish specimens. The study demonstrates the efficacy of collagen peptide mass fingerprinting in identifying different tissue types of fish samples, highlighting its importance in detecting mislabelling fraud in the food chain.
Review
Microbiology
Eva Torres-Sangiao, Cristina Leal Rodriguez, Carlos Garcia-Riestra
Summary: Mass spectrometry (MS) has emerged as a powerful tool in clinical microbiology for rapid and reliable microbial identification. Its cost- and time-effective advantages make it a promising alternative to conventional methods, offering potential applications in early diagnosis and appropriate treatment of severe infections.
Article
Medicine, General & Internal
Monique Melo Costa, Hugo Martin, Bertrand Estellon, Francois-Xavier Dupe, Florian Saby, Nicolas Benoit, Herve Tissot-Dupont, Matthieu Million, Bruno Pradines, Samuel Granjeaud, Lionel Almeras
Summary: SARS-CoV-2 diagnosis utilizing MALDI-TOF MS profiling and ML models from salivary samples is an interesting supplementary tool for COVID-19 diagnosis. Despite the mitigated results obtained for convalescent patients (D10).
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Chemistry, Analytical
Negin Fasih Ramandi, Mohammad Faranoush, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The recent success in studying the proteome as a source of biomarkers has revolutionized our understanding of leukemia. The use of mass spectrometry techniques to identify differentially expressed proteins has led to improved diagnosis, prognosis, and treatment strategies for leukemia. This review focuses on the use of various ionization sources and mass analyzers in monitoring different types of leukemia, with MALDI-TOF MS identified as a quick and reliable method for protein analysis. Additionally, the review classifies over 400 proteins and identifies specific biomarkers for acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and other stages of leukemia for future studies.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Immunology
Joanna Matysiak, Eliza Matuszewska, Marek L. Kowalski, Slawomir W. Kosinski, Ewa Smorawska-Sabanty, Jan Matysiak
Summary: Venom immunotherapy (VIT) in allergic patients is closely associated with changes in serum proteomic patterns, particularly involving complement proteins and myosin, with major proteomic changes detected in high responder group, suggesting a direct response to VIT.
Article
Hematology
Mehdi Nouraie, Deepika S. Darbari, Sohail Rana, Caterina P. Minniti, Oswaldo L. Castro, Lori Luchtman-Jones, Craig Sable, Niti Dham, Gregory J. Kato, Mark T. Gladwin, Gregory Ensing, Manuel Arteta, Andrew Campbell, James G. Taylor, Sergei Nekhai, Victor R. Gordeuk
AMERICAN JOURNAL OF HEMATOLOGY
(2020)
Article
Gastroenterology & Hepatology
Ravi Vats, Silvia Liu, Junjie Zhu, Dhanunjay Mukhi, Egemen Tutuncuoglu, Nayra Cardenes, Sucha Singh, Tomasz Brzoska, Karis Kosar, Mikhil Bamne, Jude Jonassaint, Adeola Adebayo Michael, Simon C. Watkins, Cheryl Hillery, Xiaochao Ma, Kari Nejak-Bowen, Mauricio Rojas, Mark T. Gladwin, Gregory J. Kato, Sadeesh Ramakrishnan, Prithu Sundd, Satdarshan Pal Monga, Tirthadipa Pradhan-Sundd
Article
Hematology
Victor R. Gordeuk, Binal N. Shah, Xu Zhang, Philip E. Thuma, Stenford Zulu, Rodgers Moono, N. Scott Reading, Jihyun Song, Yingze Zhang, Mehdi Nouraie, Andrew Campbell, Caterina P. Minniti, Sohail R. Rana, Deepika S. Darbari, Gregory J. Kato, Mei Niu, Oswaldo L. Castro, Roberto Machado, Mark T. Gladwin, Josef T. Prchal
AMERICAN JOURNAL OF HEMATOLOGY
(2020)
Review
Immunology
Oluwabukola T. Gbotosho, Maria G. Kapetanaki, Gregory J. Kato
Summary: Hemolysis is a common feature in various diseases, resulting in the release of harmful metabolites from hemoglobin and exceeding the capacity of protective mechanisms. In Sickle Cell Disease (SCD), circulating heme plays a significant role in disease progression by promoting oxidative stress and proinflammatory responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Letter
Hematology
Annelies J. van Vuren, Caterina P. Minniti, Laurel Mendelsohn, John H. Baird, Gregory J. Kato, Eduard J. van Beers
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Article
Medicine, General & Internal
James F. Casella, Bruce A. Barton, Julie Kanter, L. Vandy Black, Suvankar Majumdar, Adlette Inati, Yasser Wali, Richard A. Drachtman, Miguel R. Abboud, Yurdanur Kilinc, Beng R. Fuh, Murtadha K. Al-Khabori, Clifford M. Takemoto, Emad Salman, Sharada A. Sarnaik, Nirmish Shah, Claudia R. Morris, Jennifer Keates-Baleeiro, Ashok Raj, Ofelia A. Alvarez, Lewis L. Hsu, Alexis A. Thompson, India Y. Sisler, Betty S. Pace, Suzie A. Noronha, Joseph L. Lasky, Elena Cela de Julian, Kamar Godder, Courtney Dawn Thornburg, Natalie L. Kamberos, Rachelle Nuss, Anne M. Marsh, William C. Owen, Anne Schaefer, Cameron K. Tebbi, Christophe F. Chantrain, Debra E. Cohen, Zeynep Karakas, Connie M. Piccone, Alex George, Jason M. Fixler, Tammuella C. Singleton, Thomas Moulton, Charles T. Quinn, Clarisse Lopes de Castro Lobo, Abdulkareem M. Almomen, Meenakshi Goyal-Khemka, Philip Maes, Marty Emanuele, Rebecca T. Gorney, Claire S. Padgett, Ed Parsley, Shari S. Kronsberg, Gregory J. Kato, Mark T. Gladwin
Summary: The study found that poloxamer 188 did not significantly shorten the duration of vaso-occlusive episodes in children and adults with sickle cell disease. Although there were some differences observed in participants under 16 years old, the overall effect was not significant.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2021)
Review
Biochemistry & Molecular Biology
Himanshu Garg, Kristina J. Tatiossian, Karsten Peppel, Gregory J. Kato, Eva Herzog
Summary: SCD is a common monogenic disorder caused by a point mutation in the beta-globin gene, making it a prototypical target for precision therapy. Both the mutant beta-globin protein and its downstream pathophysiology are intensively researched pharmacological targets. Recent advances in HSC transplantation and gene therapy platforms have expanded potentially curative options for SCD patients.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Hematology
Solomon Johnson, Victor R. Gordeuk, Roberto Machado, J. Simon R. Gibbs, Mariana Hildesheim, Jane A. Little, Gregory J. Kato, Mark T. Gladwin, Mehdi Nouraie
Summary: This study evaluated the predictors and prognostic value of exercise-induced vital sign changes (VSC) during the six-minute walk test (6MWT) in adult sickle cell disease (SCD) patients. The findings showed that increases in heart rate and blood pressure, as well as oxygen desaturation, are common during the 6MWT for SCD patients. Additionally, an increase in systolic blood pressure (SBP) during the 6MWT was associated with improved survival, indicating potential benefits in stroke volume capacity for these patients.
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Meeting Abstract
Hematology
Varshini Babu, Jane A. Little, Claudia R. Morris, Roberto Machado, J. Simon R. Gibbs, Gregory J. Kato, Victor R. Gordeuk, Mark T. Gladwin, Yingze Zhang, Seyed Mehdi Nouraie
Article
Medicine, General & Internal
Thomas Gentinetta, John D. Belcher, Valerie Bruegger-Verdon, Jacqueline Adam, Tanja Ruthsatz, Joseph Bain, Daniel Schu, Lisa Ventrici, Monika Edler, Hadi Lioe, Kalpeshkumar Patel, Chunsheng Chen, Julia Nguyen, Fuad Abdulla, Ping Zhang, Andreas Wassmer, Meena Jain, Marcel Mischnik, Matthias Pelzing, Kirstee Martin, Roslyn Davis, Svetlana Didichenko, Alexander Schaub, Nathan Brinkman, Eva Herzog, Adrian Zuercher, Gregory M. Vercellotti, Gregory J. Kato, Gerald Hoebarth
Summary: People living with sickle cell disease (SCD) often suffer from acute pain due to blood vessel blockage. This study discovered that hemopexin, a protein found in plasma, can alleviate the symptoms of vaso-occlusion by inhibiting inflammatory reactions and reducing the expression of adhesion proteins. Animal experiments and clinical trials have shown promising results, suggesting that hemopexin could be a potential treatment for SCD.
JOURNAL OF CLINICAL MEDICINE
(2022)
Letter
Hematology
Anusha Vittal, Hawwa Alao, Julian Hercun, Bashar Sharma, Arsalan Khan, Disha Sharma, Wilson Lee, Devika Kapuria, Matthew Hsieh, John Tisdale, Courtney Fitzhugh, David Kleiner, Elliot Levy, Richard Chang, Anna Conrey, Elenita Rivera, Amy Huang, Gil Ben Yakov, Gregory J. Kato, Mark T. Gladwin, Swee Lay Thein, Christopher Koh, Theo Heller
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Review
Cell Biology
Tirthadipa Pradhan-Sundd, Gregory J. Kato, Enrico M. Novelli
Summary: Sickle cell disease is a common genetic disorder that can lead to liver dysfunction, with therapeutic options still not well understood. Animal models have played a crucial role in studying the molecular mechanisms and potential treatments for SCD-related liver diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mara Carreno, Maria F. Pires, Steven R. Woodcock, Tomasz Brzoska, Samit Ghosh, Sonia R. Salvatore, Fei Chang, Nicholas K. H. Khoo, Matthew Dunn, Nora Connors, Shuai Yuan, Adam C. Straub, Stacy G. Wendell, Gregory J. Kato, Bruce A. Freeman, Solomon F. Ofori-Acquah, Prithu Sundd, Francisco J. Schopfer, Dario A. Vitturi
Summary: The up-regulation of kynurenine metabolism leads to the production of immunomodulatory Kyn-CKA. In sickle cell disease, increased synthesis of Kyn-CKA inhibits inflammation and protects tissues from damage.
Meeting Abstract
Hematology
Svetlana Didichenko, Alexey Navdaev, Moritz Saxenhofer, Stefanie Graeter, Alexander Schaub, Alicia Rovo, Gregory J. Kato, Bronwyn Kingwell
Article
Genetics & Heredity
Mengna Zhang, William B. Hillegass, Xue Yu, Suvankar Majumdar, J. Daryl Pollard, Erin Jackson, Jarrod Knudson, Douglas Wolfe, Gregory J. Kato, Joseph F. Maher, Hao Mei
Summary: This study identified several candidate genetic variants associated with QTc interval prolongation in sickle cell disease (SCD) patients and found serum alanine transaminase (ALT) to be a modifying factor. The association of a CPS1 gene variant in both QTc and JTc duration suggests the involvement of the urea cycle and nitric oxide metabolism in cardiac repolarization in SCD. Larger replication studies are needed to confirm these findings and elucidate the underlying mechanisms.