Article
Food Science & Technology
Ludmila Kalcakova, Matej Pospiech, Bohuslava Tremlova, Zdenka Javurkova, Irina Chernukha
Summary: A newly developed immunohistochemical method for casein detection in meat products was successfully implemented with a 72% correspondence rate compared to the Enzyme-Linked Immuno Sorbent Assay (ELISA). The sensitivity and specificity of the method were determined to be 1.21 and 0.28 respectively, with the highest correspondence found in frankfurters at 90% and the lowest in canned pate at 44%.
Letter
Parasitology
Ming Li, Tianfei Yu
Summary: This study compared different detection methods for identifying different life stages of Plasmodium vivax and found issues with the consistency of the current gold standard method. It is recommended to use more appropriate statistical methods to support scientific conclusions in future research.
PARASITES & VECTORS
(2022)
Article
Plant Sciences
Tourya Sagouti, Naima Rhallabi, Giancarlo Polizzi, Abdessalem Tahiri, Zineb Belabess, Essaid Ait Barka, Rachid Lahlali
Summary: This study aims to validate serological and molecular methods for routine diagnosis of S. citri in Moroccan citrus groves. A survey was conducted in the main citrus-growing regions of Morocco, and samples were collected from citrus trees with symptoms attributable to S. citri infection. The results showed that real-time PCR assays targeting the putative adhesion-like gene P58 were the most sensitive for accurate diagnosis of S. citri.
Article
Medical Laboratory Technology
Sang Mee Hwang, Beom Joon Kim, Jee-Soo Lee, Moon-Woo Seong, Soo Hyun Seo, Jin Ho Paik, Sang-A Kim, Ji Yun Lee, Jeong-Ok Lee, Yoon Hwan Chang, Soo Mee Bang
Summary: Systemic mastocytosis with associated hematological neoplasm (SM-AHN) is not uncommon in patients with acute myeloid leukemia (AML) with RUNX1::RUNX1T1. CD117 and CD25 immunohistochemical staining play an important role in screening for SM-AHN after induction chemotherapy.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Engineering, Environmental
Shikandar D. Bukkitgar, Nagaraj P. Shetti, Tejraj M. Aminabhavi
Summary: Virus-induced infections like SARS-CoV-2 pose serious threats to human health and the world economy, necessitating rapid, accurate detection methods. The integration of electrochemical methods with nanotechnology offers effective solutions for virus detection.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Sofia Anastacio, Samuel Anjos, Suzi Neves, Tiago Neves, Pedro Esteves, Helder Craveiro, Bruno Madeira, Maria Dos Anjos Pires, Sergio Sousa, Gabriela da Silva, Hugo Vilhena
Summary: This study investigated the infection of C. burnetii in dogs, cats, and ticks in Portugal. The results showed a decrease in the exposure to C. burnetii in cats and dogs, with rural habitats favoring the infection. Additionally, no C. burnetii DNA was detected in ticks from the tested samples.
Review
Agriculture, Multidisciplinary
Mahsa Motshakeri, Manisha Sharma, Anthony R. J. Phillips, Paul A. Kilmartin
Summary: This review summarizes the application of electrochemical methods in quantifying various analytes in milk over the past 10-15 years. It covers a wide range of analytes and their corresponding detection tools, discusses pretreatment procedures and the use of novel sensor materials, and provides perspectives on future development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Medicine, General & Internal
Lusine Yaghjyan, Yujing J. Heng, Gabrielle M. Baker, Vanessa Bret-Mounet, Divya Murthy, Matt B. Mahoney, Yi Mu, Bernard Rosner, Rulla M. Tamimi
Summary: This study compared the assessment of stem cell markers CD44, CD24, and ALDH1A1 in breast tissue of cancer-free women by pathologists and automated image analysis. The results showed variable correlations between manual and computational assessments, suggesting the use of computational platforms for evaluating stem cell markers in large-scale epidemiological studies.
FRONTIERS IN MEDICINE
(2022)
Article
Biophysics
Yang Wang, Gaolian Xu, Mengdi Guan, Qingyang Zhang, Zhiying Wang, Wenhui Chen, Xiaoxiao Yang, Anbang Qiao, Yubo Fan, Xinxia Cai, Zhou Chen, Lingqian Chang, Bo Wei
Summary: An electrochemical quantitative polymerase chain reaction (E-PCR) platform was developed for multiplexed and quantitative analysis of single-based mutations (SbM) in limited and precious samples, achieving ultra-high sensitivity and specificity.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Applied
Anne Eischeid, Rakhi Panda, Chung Cho, Sarah Stadig
Summary: Increased interest in consumption of insects has raised concerns about food safety, particularly regarding allergies. This study aimed to investigate if clinical cross-reactivity between crustacean shellfish and insects translates into analytical cross-reactivity with allergen detection assays. The results showed that immunoassay-based methods were prone to cross-reactivity, leading to potential false positive detection of crustacean allergens in insect samples. However, DNA-based PCR methods had minimal reactivity with insects, providing clearer results.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Review
Veterinary Sciences
Joao Paulo de Lacerda Roberto, Clecio Henrique Limeira, Nathanael Natercio da Costa Barnabe, Rafael Rodrigues Soares, Maria Luana Cristiny Rodrigues Silva, Alberio Antonio de Barros Gomes, Severino Silvano dos Santos Higino, Sergio Santos de Azevedo, Clebert Jose Alves
Summary: Paratuberculosis is an incurable infectious disease affecting various species, including goats, caused by Mycobacterium avium subspecies paratuberculosis. The study aimed to conduct a systematic review and meta-analysis of prevalence and diagnostic tests for detecting MAP antibodies and DNA in goat milk, revealing significant variability in infection rates and detection methods.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
Alison Devonshire, Gerwyn Jones, Ana Fernandez Gonzalez, Olga Kofanova, Johanna Trouet, Pamela Pinzani, Stefania Gelmini, Serena Bonin, Carole Foy
Summary: The analysis of circulating cell-free DNA (ccfDNA) in liquid biopsies is becoming increasingly important in clinical practice. However, the accuracy of diagnosis is greatly impacted by the quality of the samples, and standardized approaches for assessing the quality of ccfDNA have not yet been established. This study evaluates the use of nucleic acid spike-in control materials as a quality control (QC) and standardization method for cfDNA isolation in in vitro diagnostic assays.
Article
Infectious Diseases
Pengfei Cai, Yi Mu, Kosala G. Weerakoon, Remigio M. Olveda, Allen G. Ross, Donald P. McManus
Summary: The POC-CCA test showed sensitivity/specificity values of 63.3%/93.3% for detecting individuals infected with S. japonicum with high egg burden, but its performance was limited in diagnosing infections with low egg burden. Therefore, caution should be exercised when applying this test in populations with low intensity S. japonicum infections.
INFECTIOUS DISEASES OF POVERTY
(2021)
Article
Agriculture, Dairy & Animal Science
David Conrad, Alexandra Kehl, Tobias Mueller, Robert Klopfleisch, Heike Aupperle-Lellbach
Summary: This study evaluated the prognosis and treatment of canine cutaneous mast cell tumours in the toe, finding that over half of the tumours had poor prognosis indicators and mutations in the c-kit gene. French Bulldogs were more likely to develop malignant tumours. This study contributes to better understanding of digital mast cell tumours in canines.
Article
Multidisciplinary Sciences
Jiyeon An, Ha Young Woo, Younghan Lee, Hyo Song Kim, Juhyeon Jeong, Sang Kyum Kim
Summary: Desmoid-type fibromatosis (DF) is a locally aggressive neoplasm characterized by mutations in the CTNNB1 gene, with CTNNB1-mutant DF samples consistently displaying nuclear beta-catenin expression. Larger or excised DF tumors with CTNNB1 mutations tend to exhibit nuclear beta-catenin expression, and males show a higher frequency of T41A or T41I mutations.
