Journal
EXPERT OPINION ON DRUG DISCOVERY
Volume 6, Issue 2, Pages 141-153Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1517/17460441.2011.546396
Keywords
drug discovery; drug screening; electrophysiology; GPCR; ligand-gated ion channel; transporter; two-electrode voltage-clamp; voltage-gated ion channel; Xenopus oocytes
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Funding
- University of Copenhagen
- Augustinus Foundation
- Lundbeck Foundation
- Villum Kann Rasmussen Foundation
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Areas covered: This review summarizes the use of Xenopus oocytes in drug screening, presents the advantages and disadvantages of the use of Xenopus oocytes as expression system, and addresses the options available for automated two-electrode voltage-clamp recordings from Xenopus oocytes. Expert opinion: Automated and manual Xenopus oocyte two-electrode voltage-clamp recordings are useful and important techniques in drug screening. Although they are not compatible with high-throughput experimentation, these techniques are excellent in combination or as alternatives to fluorescence-based assays for hit validation, screening of focused compound libraries and safety screening on ion channels with their high flexibility for the choice of molecular targets, quality of data and reproducibility.
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