Article
Agronomy
Piotr Ochodzki, Adriana Twardawska, Halina Wisniewska, Tomasz Goral
Summary: The study identified winter wheat lines with low levels of head infection and kernel damage as well as low levels of grain contamination with mycotoxins.
Article
Food Science & Technology
Pascaline Aimee Uwineza, Monika Urbaniak, Marcin Bryla, Lukasz Stepien, Marta Modrzewska, Agnieszka Waskiewicz
Summary: This research aimed to extract lemon balm using supercritical CO2 and methanol, and evaluate its antifungal activity against Fusarium species. The results showed that lemon balm extracts inhibited the growth and mycotoxin synthesis of Fusarium strains, supporting their potential as natural alternatives to synthetic fungicides. However, more research is needed on other Fusarium species and in vivo applications.
Article
Food Science & Technology
Elizabet Janic Hajnal, Janja Babic, Lato Pezo, Vojislav Banjac, Radmilo Colovic, Jovana Kos, Jelena Krulj, Katarina Pavsic-Vrtac, Breda Jakovac-Strajn
Summary: This study investigated the effects of extrusion processing parameters on the reduction of various mycotoxins in whole grain triticale flour. The optimal conditions for lowering toxin concentrations were found to be specific screw speed, feed rate, and moisture content. This study provides insights on the fate of mycotoxins during extrusion processing.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Marcin Bryla, Adam Pierzgalski, Agnieszka Zapasnik, Pascaline Aimee Uwineza, Edyta Ksieniewicz-Wozniak, Marta Modrzewska, Agnieszka Waskiewicz
Summary: This review summarizes the latest research on the toxic secondary metabolites of Fusarium in maize, including modified forms of mycotoxins, the research progress on the health effects, and the development of biological methods to eliminate the presence of mycotoxins.
Article
Food Science & Technology
Saara Sammalisto, Miikka Laitinen, Kati Katina, Tuula Sontag-Strohm
Summary: This study investigates the impact of hydrothermal treatment parameters on oat groat, flake, and flour properties. The results show that tempering temperature significantly affects groat hardness and flour particle size, with differences observed among different samples. Additionally, moisture content also influences groat hardness and flour particle size. Therefore, by selecting appropriate oat varieties and adjusting treatment parameters, the milling properties of oat can be optimized.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
S. A. Palacios, A. Del Canto, J. Erazo, A. M. Torres
Summary: This study evaluated the aggressiveness of F. cerealis to durum wheat cultivars and mycotoxin production in planta, finding that all cultivars exhibited typical FHB symptoms and NIV was the most produced mycotoxin. Molecular analysis revealed all strains possessed NIV genotype, while chemical assessment showed they could produce various secondary metabolites. The increasing incidence of F. cerealis and the potential crop contamination with mycotoxins pose great concern for food security and world cereal trade, especially given the higher toxicity of NIV compared to DON.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Xenia Pascari, Sonia Marin, Antonio J. Ramos, Vicente Sanchis
Summary: This review discusses the possible infection of cereals used in beer production by Fusarium fungi and the transfer of mycotoxins during malting and brewing. Studies show that germination and mashing processes have a significant impact on mycotoxin levels, while fermentation can slightly reduce their content. Additionally, limited research is available on mycotoxin occurrence in craft beer.
Article
Biochemistry & Molecular Biology
Mario Masiello, Stefania Somma, Chiara Lo Porto, Fabio Palumbo, Pietro Favia, Francesco Fracassi, Antonio Francesco Logrieco, Antonio Moretti
Summary: Contamination of maize by mycotoxin-producing Fusarium species is a global concern. The ability of F. proliferatum and F. graminearum to translocate within maize plants is poorly understood. Plasma-generated coatings with embedded prothioconazole show promise in protecting maize seedlings from Fusarium infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Li Li, Peng Li, Yu Wu, Jin Ye, Zongwang Li, Songxue Wang
Summary: Matrix certified reference materials are crucial for analytical method validation and reliable measurement results. A certified reference material for whole-wheat flour has been developed to accurately measure the main Fusarium mycotoxins. This CRM is homogeneous, stable, and suitable for quality control and method validation of mycotoxin quantification in whole-wheat flour.
Editorial Material
Microbiology
Lukasz Stepien
Summary: Fusarium species are widespread fungi with both saprotrophic and pathogenic properties. They are also known for their ability to produce mycotoxins, which makes them highly damaging to plants. Molecular tools have been valuable in species identification and phylogeny studies of the Fusarium genus.
Article
Chemistry, Applied
Nela Prusova, Zbynek Dzuman, Lukas Jelinek, Marcel Karabin, Jana Hajslova, Michael Rychlik, Milena Stranska
Summary: The study investigated the fate of less investigated Fusarium and Alternaria mycotoxins during the malting and brewing processes of producing lager beer. Results showed that germination is the most determining malting step, and mitigation measures are needed to reduce mycotoxins contamination in the final beer product.
Article
Agronomy
Daniela Simoes, Eugenio Diogo, Eugenia de Andrade
Summary: Maize is an important staple crop worldwide, but it is highly susceptible to fungal infections, particularly by certain species of Fusarium. These infections can lead to significant reductions in yield and quality, as well as the production of mycotoxins. In Portugal and similar Mediterranean countries, F. verticillioides and F. subglutinans are the most common species infecting maize. However, the incidence of diseases and mycotoxin levels have increased, suggesting the presence of other mycotoxigenic Fusarium species or changes in exogenous conditions. Monitoring Fusarium species occurrence in the field is crucial to address these threats. A recent survey in Portugal identified the presence of F. andiyazi in maize crops for the first time, highlighting the need for further studies to prevent its spread and mitigate its impact on maize production and farmers' livelihoods.
Article
Engineering, Chemical
Sebastian Birkedal Kristensen, Mahdi Nikbakht Fini, Tobias Bruun Pedersen, Jens Laurids Sorensen, Jens Muff
Summary: This study focused on investigating the separation, concentration, and purification possibilities of valuable fungal pigments, produced by Fusarium solani, using membrane filtration. It was found that membrane filtration can effectively separate, concentrate, and purify the investigated fusarubins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Microbiology
Lakshmipriya Perincherry, Natalia Witaszak, Monika Urbaniak, Agnieszka Waskiewicz, Lukasz Stepien
Summary: The study focused on identifying differentially expressed secondary metabolites in resistant and susceptible cultivars of pea and their impact on the growth and mycotoxin biosynthesis of two different Fusarium species. It was found that certain metabolites could inhibit the pathogen's growth, while a higher level of one metabolite actually enhanced the growth of a specific Fusarium strain. Additionally, these metabolites were effective in inhibiting the biosynthesis of certain mycotoxins, regardless of the pathogen's ability to produce toxins.
Article
Agronomy
Valentina Spanic, Marko Maricevic, Ivica Ikic, Michael Sulyok, Hrvoje Sarcevic
Summary: Fusarium head blight (FHB) is a fungal disease that causes economic losses and reduces grain quality in cereals, such as wheat. The study analyzed 216 wheat samples infected with FHB using LC-MS/MS and found that certain mycotoxins/metabolites, including deoxynivalenol (DON) and zearalenone (ZEN), were highly prevalent. The co-occurrence of mycotoxins/metabolites was high, and their potential toxic effects should be considered. The results highlight the importance of analyzing modified and emerging mycotoxins/metabolites in future mycotoxin monitoring programs.
Article
Agriculture, Multidisciplinary
Marcin Bryla, Sylwia Stepniewska, Marta Modrzewska, Agnieszka Waskiewicz, Grazyna Podolska, Edyta Ksieniewicz-Wozniak, Tomoya Yoshinari, Lukasz Stepien, Monika Urbaniak, Marek Roszko, Romuald Gwiazdowski, Joanna Kanabus, Adam Pierzgalski
Summary: This study evaluated the resistance of six cultivars of winter wheat to Fusarium head blight (FHB) and investigated the presence of deoxynivalenol (DON) and nivalenol (NIV) and their derivatives in the ears. High relative humidity above 80% was found to be a decisive factor for FHB incidence. All wheat cultivars showed the ability to convert DON and NIV into glucosides. These findings are important for understanding the extent of glucosylation of trichothecenes in different wheat cultivars.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Pascaline Aimee Uwineza, Monika Urbaniak, Marcin Bryla, Lukasz Stepien, Marta Modrzewska, Agnieszka Waskiewicz
Summary: This research aimed to extract lemon balm using supercritical CO2 and methanol, and evaluate its antifungal activity against Fusarium species. The results showed that lemon balm extracts inhibited the growth and mycotoxin synthesis of Fusarium strains, supporting their potential as natural alternatives to synthetic fungicides. However, more research is needed on other Fusarium species and in vivo applications.
Article
Biochemistry & Molecular Biology
Magdalena Jeszka-Skowron, Agnieszka Zgola-Grzeskowiak, Robert Frankowski, Tomasz Grzeskowiak, Anna Maria Jeszka
Summary: This study determined the content of active compounds in the roots, stems, and leaves of nettle and found trigonelline in all parts of the plant. Principal Component Analysis showed differences in the content of bioactive components between the roots and aerial parts of nettle.
Article
Chemistry, Multidisciplinary
Marta Wozniak-Karczewska, Anna Parus, Tomasz Ciesielski, Artur Trzebny, Radoslaw Szumski, Wiktoria Wilms, Jan Homa, Grzegorz Framski, Daniel Baranowski, Robert Frankowski, Agnieszka Zgola-Grzeskprime Owiak, Michal Niemczak, Miroslawa Dabert, Andras Tancsics, Lukasz Chrzanowski
Summary: This study aims to increase the hydrophobicity of herbicides by pairing them with betaine and modified betaine zwitterions. The research results show that the adsorption rate of hydrophobic ions is high, while the adsorption rate of hydrophilic ions is low. Additionally, hydrophobic surface-active derivatives of betaines play a significant role in environmental and plant toxicity.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Rafal Krakowiak, Robert Frankowski, Kinga Mylkie, Dariusz T. Mlynarczyk, Marta Ziegler-Borowska, Agnieszka Zgola-Grzeskowiak, Tomasz Goslinski
Summary: A composite material based on titanium(IV) oxide and iron(II,III) oxide was prepared and characterized in this study, showing lower photocatalytic activity compared to bare TiO2. The material exhibited reduced acute toxicity in Microtox assay, but its recovery after photocatalytic tests was limited by a side oxidation reaction of Fe3O4.
APPLIED SCIENCES-BASEL
(2022)
Article
Food Science & Technology
Agnieszka Waskiewicz, Malgorzata Muzolf-Panek, Lukasz Stepien, Elzbieta Czembor, Pascaline Aimee Uwineza, Pawel Gornas, Marcin Bryla
Summary: This study analyzed the resistance of four sweet maize cultivars to infection with Fusarium isolates and their mycotoxin contamination. The results showed variations in ear rot levels among different cultivars and isolates. Additionally, the concentrations of fumonisins were positively correlated with the phenotypic evaluation.
Article
Biochemistry & Molecular Biology
Justyna Lalak-Kanczugowska, Natalia Witaszak, Agnieszka Waskiewicz, Jan Bocianowski, Lukasz Stepien
Summary: Fusarium proliferatum is a common hemi-biotrophic pathogen that infects a wide range of host plants, leading to significant crop losses. In this study, the effects of selected plant metabolites on the metabolism of F. proliferatum were analyzed. The results showed that some metabolites promoted fungal growth while others inhibited it.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marta Modrzewska, Lidia Blaszczyk, Lukasz Stepien, Monika Urbaniak, Agnieszka Waskiewicz, Tomoya Yoshinari, Marcin Bryla
Summary: This study evaluates the inhibitory effect of selected strains of Trichoderma viride, T. viridescens, and T. atroviride on the growth and mycotoxin production of Fusarium culmorum and F. cerealis. The results show that Trichoderma strains significantly inhibit the mycelium growth of Fusarium and can colonize the medium surface, grow over the pathogen's mycelium, and sporulate. When co-cultured on rice, Trichoderma strains also exhibit inhibitory effects on the biosynthesis of mycotoxins. Additionally, Trichoderma is capable of glycosylating DON and converting ZEN into its hydroxylated derivative, beta-ZOL.
Article
Agriculture, Dairy & Animal Science
Marcin Bryla, Krzysztof Damaziak, Magdalena Twaruzek, Agnieszka Waskiewicz, Lukasz Stepien, Marek Roszko, Adam Pierzgalski, Ewelina Soszczynska, Monika Lukasiewicz-Mierzejewska, Marta Chmiel, Wojciech Wojcik
Summary: In this study, the embryotoxicity of ochratoxin A (OTA) and its diastereomer 2'R-ochratoxin A (2'R-OTA) was compared under in ovo conditions, and the in vitro embryotoxicity of these substances together with ochratoxin B and a-ochratoxin was assessed using chicken embryo cell lines. The results showed that both OTA and 2'R-OTA demonstrated embryotoxicity, but the highest dose of OTA diastereomer resulted in higher chick hatchability compared to the group that received OTA. The concentrations of OTA were higher than 2'R-OTA in chicken organs such as liver and kidney, while 2'ROTA concentrations were higher in blood serum and heart.
Article
Engineering, Chemical
Robert Frankowski, Agnieszka Zgola-Grzeskowiak, Tomasz Grzeskowiak, Ewa Stanisz, Justyna Werner, Julia Platkiewicz
Summary: This study investigates the photodegradation of bisphenol A, bisphenol S, and fluconazole using UV light and different catalysts. The results show that photocatalytic removal of these compounds is challenging, but the modification of TiO2 with silver improves the degradation of bisphenols. However, the degradation of fluconazole remains low.
Article
Biochemistry & Molecular Biology
Magdalena Jeszka-Skowron, Robert Frankowski, Agnieszka Zgola-Grzeskowiak, Julia Platkiewicz
Summary: This study analyzed 49 coffee samples of Arabica and Robusta species from different geographical origins and with different beans treatment. The study compared the metabolites of Kopi Luwak coffee with fully washed coffees and the drying post-harvest treatment of Arabica or Robusta coffee brews. The results showed that Kopi Luwak had higher levels of caffeine and theophylline compared to the analyzed washed and unwashed Arabica coffees, and a different proportion of caffeoylquinic isomers. There was no difference in the concentration of vitamin B-3, amines, and phenolic acids between Kopi Luwak and other Arabica coffees.
Editorial Material
Microbiology
Lukasz Stepien
Summary: Fusarium species are widespread fungi with both saprotrophic and pathogenic properties. They are also known for their ability to produce mycotoxins, which makes them highly damaging to plants. Molecular tools have been valuable in species identification and phylogeny studies of the Fusarium genus.
Article
Microbiology
Lidia Blaszczyk, Hanna Cwiek-Kupczynska, Karolina Hoppe Gromadzka, Aneta Basinska-Barczak, Lukasz Stepien, Joanna Kaczmarek, Leszek Lenc
Summary: The antagonistic potential of 33 Clonostachys and Trichoderma strains was evaluated in this study, and C. rosea AN291, T. atroviride AN240, and T. viride AN430 were found to be the most effective in inhibiting the growth of F. culmorum and the synthesis of mycotoxins. These strains also exhibited mycoparasitic abilities against KF846 and the ability to eliminate F. culmorum toxins.
Article
Biochemistry & Molecular Biology
Tomasz Tronina, Mateusz Luzny, Monika Dymarska, Monika Urbaniak, Ewa Kozlowska, Michal Piegza, Lukasz Stepien, Tomasz Janeczko
Summary: Quercetin, the most abundant flavonoid in food products, has various biological activities and is used for medical applications. However, its low water solubility may limit its therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Pascaline Aimee Uwineza, Monika Urbaniak, Lukasz Stepien, Anna Gramza-Michalowska, Agnieszka Waskiewicz
Summary: This study evaluated the efficacy of L. album flower extracts in inhibiting the in vitro growth and biosynthesis of mycotoxins by Fusarium culmorum and F. proliferatum strains. The results showed that L. album flower extracts significantly reduced mycelium growth and mycotoxin synthesis. These findings are important for further assessing the fungistatic effect of L. album flower extracts on cereals and developing bio-preparations to protect cereals against Fusarium infections.
