New developments in lentiviral vector design, production and purification

Bystander killing of malignant cells via the delivery of engineered thymidine-active deoxycytidine kinase for suicide gene therapy of cancer

(2012) A Neschadim et al.   CANCER GENE THERAPY

Medium Optimization for Exopolysaccharide Production in Liquid Culture of Endophytic Fungus Berkleasmium sp. Dzf12

(2012) Peiqin Li et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Generation of Human ^|^beta;-thalassemia Induced Pluripotent Stem Cells from Amniotic Fluid Cells Using a Single Excisable Lentiviral Stem Cell Cassette

(2012) Yong FAN et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

The enhancement of RNAi against HIV in vitro and in vivo using H-2Kk protein as a sorting method

(2012) Ying Gu et al.   JOURNAL OF VIROLOGICAL METHODS

Virus-producing cells determine the host protein profiles of HIV-1 virion cores

(2012) Steven Santos et al.   Retrovirology

Improvement of lentiviral transfer vectors using cis-acting regulatory elements for increased gene expression

(2011) Gonçalo Real et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells

(2011) Melanie Wurm et al.   BIOLOGICAL CHEMISTRY

Aggregates in monoclonal antibody manufacturing processes

(2011) María Vázquez-Rey et al.   BIOTECHNOLOGY AND BIOENGINEERING

Construction of stable producer cells to make high-titer lentiviral vectors for dendritic cell-based vaccination

(2011) Chi-Lin Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production

(2011) A.F. Rodrigues et al.   BIOTECHNOLOGY AND BIOENGINEERING

Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture

(2011) Xinghui Zhao et al.   BIOTECHNOLOGY LETTERS

Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration

(2011) Katrin Zimmermann et al.   BMC BIOTECHNOLOGY

Downstream processing of cell culture-derived virus particles

(2011) Michael W Wolf et al.   Expert Review of Vaccines

Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors

(2011) H P Lesch et al.   GENE THERAPY

Lentivirus Production Is Influenced by SV40 Large T-Antigen and Chromosomal Integration of the Vector in HEK293 Cells

(2011) Leonor Gama-Norton et al.   HUMAN GENE THERAPY

Lentiviral Vectors: Their Molecular Design, Safety, and Use in Laboratory and Preclinical Research

(2011) Boro Dropulić   HUMAN GENE THERAPY

Efficient Large Volume Lentiviral Vector Production Using Flow Electroporation

(2011) Scott R. Witting et al.   HUMAN GENE THERAPY

Highly efficient large-scale lentiviral vector concentration by tandem tangential flow filtration

(2011) Aaron R. Cooper et al.   JOURNAL OF VIROLOGICAL METHODS

Abrogation of contaminating RNA activity in HIV-1 Gag VLPs

(2011) Ziyaad Valley-Omar et al.   Virology Journal

Lentiviral Vectors in Gene Therapy: Their Current Status and Future Potential

(2010) David Escors et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Bioprocessing of Baculovirus Vectors: A Review

(2010) Marc G. Aucoin et al.   CURRENT GENE THERAPY

Large-Scale Production Means for the Manufacturing of Lentiviral Vectors

(2010) M. Schweizer et al.   CURRENT GENE THERAPY

Improvement of recombinant protein production by an anti-apoptotic protein from hemolymph of Lonomia obliqua

(2010) Helena L. A. Vieira et al.   CYTOTECHNOLOGY

Improvement of lentiviral vector-mediated gene transduction by genetic engineering of the structural protein Pr55Gag

(2010) T Aoki et al.   GENE THERAPY

Large-Scale Manufacture and Characterization of a Lentiviral Vector Produced for Clinical Ex Vivo Gene Therapy Application

(2010) Otto-Wilhelm Merten et al.   HUMAN GENE THERAPY

A Stable Producer Cell Line for the Manufacture of a Lentiviral Vector for Gene Therapy of Parkinson's Disease

(2010) Hannah J. Stewart et al.   HUMAN GENE THERAPY

Creating Higher Titer Lentivirus with Caffeine

(2010) Brian L. Ellis et al.   HUMAN GENE THERAPY

Affinity recovery of lentivirus by diaminopelargonic acid mediated desthiobiotin labelling

(2010) Rongjun Chen et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Transduction of Human Primitive Repopulating Hematopoietic Cells With Lentiviral Vectors Pseudotyped With Various Envelope Proteins

(2010) Yoon-Sang Kim et al.   MOLECULAR THERAPY

Toward Gene Therapy for Cystic Fibrosis Using a Lentivirus Pseudotyped With Sendai Virus Envelopes

(2010) Katsuyuki Mitomo et al.   MOLECULAR THERAPY

Advances in the Field of Lentivector-based Transduction of T and B Lymphocytes for Gene Therapy

(2010) Cecilia Frecha et al.   MOLECULAR THERAPY

Generation of Transgene-Free Lung Disease-Specific Human Induced Pluripotent Stem Cells Using a Single Excisable Lentiviral Stem Cell Cassette

(2010) Aba Somers et al.   STEM CELLS

A novel animal-component-free medium for rabies virus production in Vero cells grown on Cytodex 1 microcarriers in a stirred bioreactor

(2009) Samia Rourou et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production of Germline Transgenic Prairie Voles (Microtus ochrogaster) Using Lentiviral Vectors1

(2009) Zoe R. Donaldson et al.   BIOLOGY OF REPRODUCTION

Mcl-1 overexpression leads to higher viabilities and increased production of humanized monoclonal antibody in Chinese hamster ovary cells

(2009) Brian S. Majors et al.   BIOTECHNOLOGY PROGRESS

Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells

(2009) T. VandenDriessche et al.   BLOOD

Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection

(2009) R. E. Throm et al.   BLOOD

Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography

(2009) Robert H Kutner et al.   BMC BIOTECHNOLOGY

Development of inducible EIAV-based lentiviral vector packaging and producer cell lines

(2009) H J Stewart et al.   GENE THERAPY

The genotoxic potential of retroviral vectors is strongly modulated by vector design and integration site selection in a mouse model of HSC gene therapy

(2009) Eugenio Montini et al.   JOURNAL OF CLINICAL INVESTIGATION

Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer

(2009) Marlene Carmo et al.   JOURNAL OF GENE MEDICINE

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

(2009) Sven Ansorge et al.   JOURNAL OF GENE MEDICINE

Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection

(2009) Hitoshi Kuroda et al.   JOURNAL OF VIROLOGICAL METHODS

Analysis of the Viral Elements Required in the Nuclear Import of HIV-1 DNA

(2009) L. Riviere et al.   JOURNAL OF VIROLOGY

Optimized Lentiviral Vector Design Improves Titer and Transgene Expression of Vectors Containing the Chicken β-Globin Locus HS4 Insulator Element

(2009) Hideki Hanawa et al.   MOLECULAR THERAPY

Hybrid Lentivirus-transposon Vectors With a Random Integration Profile in Human Cells

(2009) Nicklas H Staunstrup et al.   MOLECULAR THERAPY

Insertional Transformation of Hematopoietic Cells by Self-inactivating Lentiviral and Gammaretroviral Vectors

(2009) Ute Modlich et al.   MOLECULAR THERAPY

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

(2009) Lajos Mátés et al.   NATURE GENETICS

Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors

(2009) Robert H Kutner et al.   Nature Protocols

A Lentivirus-Mediated Genetic Screen Identifies Dihydrofolate Reductase (DHFR) as a Modulator of β-Catenin/GSK3 Signaling

(2009) Richard A. Klinghoffer et al.   PLoS One

Quantitative proteomic analysis of lentiviral vectors using 2-DE

(2009) Jérôme Denard et al.   PROTEOMICS

Hematopoietic Stem Cell Gene Therapy with a Lentiviral Vector in X-Linked Adrenoleukodystrophy

(2009) N. Cartier et al.   SCIENCE

Dopamine Gene Therapy for Parkinson's Disease in a Nonhuman Primate Without Associated Dyskinesia

(2009) B. Jarraya et al.   Science Translational Medicine

Polycistronic Lentiviral Vector for “Hit and Run” Reprogramming of Adult Skin Fibroblasts to Induced Pluripotent Stem Cells

(2009) Chia-Wei Chang et al.   STEM CELLS

Generation of Domestic Transgenic Cloned Kittens Using Lentivirus Vectors

(2009) Martha C. Gómez et al.   Cloning Stem Cells

Polyethylenimine-Based Transfection Method as a Simple and Effective Way to Produce Recombinant Lentiviral Vectors

(2008) Jorge R. Toledo et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhancement of transient gene expression and culture viability using Chinese hamster ovary cells overexpressing Bcl-xL

(2008) Brian S. Majors et al.   BIOTECHNOLOGY AND BIOENGINEERING

Sequential Expression of Pluripotency Markers during Direct Reprogramming of Mouse Somatic Cells

(2008) Tobias Brambrink et al.   Cell Stem Cell

Generation of lentivirus vectors using recombinant baculoviruses

(2008) H P Lesch et al.   GENE THERAPY

Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies

(2008) Alois Jungbauer et al.   JOURNAL OF CHROMATOGRAPHY A

Immobilized metal affinity chromatography of histidine-tagged lentiviral vectors using monolithic adsorbents

(2008) M.C. Cheeks et al.   JOURNAL OF CHROMATOGRAPHY A

Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID-X1 patients

(2008) Steven J. Howe et al.   JOURNAL OF CLINICAL INVESTIGATION

Substitution of the myristoylation signal of human immunodeficiency virus type 1 Pr55Gag with the phospholipase C- 1 pleckstrin homology domain results in infectious pseudovirion production

(2008) E. Urano et al.   JOURNAL OF GENERAL VIROLOGY

Removal of envelope protein-free retroviral vectors by anion-exchange chromatography to improve product quality

(2008) Teresa Rodrigues et al.   JOURNAL OF SEPARATION SCIENCE

Zinc-finger Nucleases: The Next Generation Emerges

(2008) Toni Cathomen et al.   MOLECULAR THERAPY

Inducible Packaging Cells for Large-scale Production of Lentiviral Vectors in Serum-free Suspension Culture

(2008) Sophie Broussau et al.   MOLECULAR THERAPY

HIV-1 matrix protein p17 induces human plasmacytoid dendritic cells to acquire a migratory immature cell phenotype

(2008) S. Fiorentini et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette

(2008) Cesar A. Sommer et al.   STEM CELLS