Article
Neurosciences
Abhishek Pethe, Mira Hamze, Marina Giannaki, Bernd Heimrich, Igor Medina, Anna-Maria Hartmann, Eleni Roussa
Summary: This study investigates the effects of KCC2/NBCe1 interaction on the phosphorylation pattern and function of KCC2. The results show that KCC2 and NBCe1 interact in the mouse brain and this interaction may have pathophysiological relevance.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Inyeong Choi, Hansoo Yang, Eunjin Kim, Soojung Lee
Summary: In this study, the researchers found that the channel activity of the sodium bicarbonate cotransporter NBCn1 decreases the function of NMDA receptors (NMDARs), and this effect may involve the interaction between NBCn1 and NMDARs. Furthermore, the results suggest that NBCn1 has dual effects on NMDARs in the brain, stabilizing receptor expression while decreasing receptor function.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Marc Severin, Emma Lind Pedersen, Magnus Thane Borre, Ida Axholm, Frederik Bendix Christiansen, Muthulakshmi Ponniah, Dominika Czaplinska, Tanja Larsen, Luis Angel Pardo, Stine Falsig Pedersen
Summary: We found that the localization of NBCn1 in epithelial cells is regulated by a predicted N-terminal beta-sheet and short C-terminal alpha-helical motif. NBCn1 interacts with E-cadherin, DLG1, and RhoA, and localizes to adherens junctions, lamellipodia, filopodia, centrosomes, and primary cilia. Our findings suggest that NBCn1 plays a role in cell cycle progression by trafficking between lateral junctions, the leading edge, and cell division machinery.
JOURNAL OF CELL SCIENCE
(2023)
Review
Chemistry, Medicinal
Jingjing Wang, Aqeela Zahra, YunFu Wang, Jianping Wu
Summary: Acid-base homeostasis is crucial for physiological function and pathology. NBCn1, a transmembrane transporter, plays a significant role in cellular pH regulation and HCO3- transport. However, the expression and activity of NBCn1 remain unclear. Furthermore, NBCn1 is involved in various cellular processes. This review provides an overview of recent research on NBCn1, including its basic features, regulation, tissue-specific physiology, and potential application in cancer therapy.
Article
Physiology
Osamu Yamazaki, Maho Yamashita, Jinping Li, Fumika Ochiai-Homma, Tadashi Yoshida, Junichi Hirahashi, Taiji Furukawa, Ken Kozuma, Yoshihide Fujigaki, George Seki, Matsuhiko Hayashi, Shigeru Shibata
Summary: This study identified the I551F variant of NBCe1-A gene, which showed impaired transport activity predominantly through cytoplasmic retention and could exert a dominant negative effect on wild-type NBCe1-A, suggesting its physiological significance.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Cell Biology
De-Jian Liu, Cui-Cui Liu, Xiu-Qin Zhong, Xuan Wu, Hui-Hui Zhang, Shang-Wen Lu, Zhuo-Ling Shen, Wen-Wen Song, Shi-Long Zhao, You-Song Peng, He-Ping Zheng, Mu-Yang Wan, Yao-Qing Chen, Lei Deng
Summary: Neuraminidase is identified as a crucial element in the development of a universal influenza vaccine. Inducing neuraminidase-specific broadly protective antibodies through vaccinations is difficult. In this study, conserved peptides from the globular head domains of neuraminidase were selected to focus the immune response and enhance cross-protections. The peptide-based sequential immunization strategy proved to be effective in inducing targeted and cross-protective antibody response, providing insights for designing universal vaccines against highly variable pathogens.
Article
Multidisciplinary Sciences
Minrui Fan, Jianxiu Zhang, Chien-Ling Lee, Jinru Zhang, Liang Feng
Summary: The sodium-chloride cotransporter (NCC) is crucial for kidney function and blood pressure regulation. This study used cryo-electron microscopy to determine the structure of human NCC and its interaction with a commonly prescribed thiazide diuretic. The findings provide important insights into the NCC's transport mechanism, as well as its regulation and interaction with thiazide diuretics, which are widely used for treating hypertension.
Article
Biochemical Research Methods
Delong Zhou, Yvan Tran, Sherif Abou Elela, Michelle S. Scott
Summary: SAPFIR is a predictor of protein features associated with alternative splicing events, allowing for rapid identification of functional alternative splicing events and analysis of proteins generated from diverse genes, providing guidance on the impact on cellular functions.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Jacob E. Pruett, Edgar D. Torres Fernandez, Steven J. Everman, Ruth M. Vinson, Kacey Davenport, Madelyn K. Logan, Stephanie A. Ye, Damian G. Romero, Licy L. Yanes Cardozo
Summary: This study investigated the therapeutic effects of empagliflozin on polycystic ovary syndrome (PCOS), showing reductions in obesity and blood pressure, and a decrease in the activity of the renin-angiotensin system. However, the drug had limited effects on blood glucose and renal injury, suggesting the need for additional treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gabriela Garcia-Rodriguez, Daniel Charlier, Dorien Wilmaerts, Jan Michiels, Remy Loris
Summary: The toxin RnlA is tightly regulated by the antitoxin RnlB through structural transitions, highlighting the crucial role of conformational heterogeneity in protein function.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Physiology
Olha Kravtsova, Ruslan Bohovyk, Vladislav Levchenko, Oleg Palygin, Christine A. Klemens, Timo Rieg, Alexander Staruschenko
Summary: This study demonstrates that Na+-glucose cotransporter-2 (SGLT2) inhibition in a nondiabetic model of salt-sensitive hypertension attenuates the development and magnitude of salt-induced hypertension. Chronic inhibition of SGLT2 increases glucose and Na+ excretion without affecting the expression and function of other Na+ transporters and channels along the nephron and hormone levels in the renin-angiotensin-aldosterone system.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Jacob E. Pruett, Steven J. Everman, Ngoc H. Hoang, Faridah Salau, Lucy C. Taylor, Kristin S. Edwards, Jonathan P. Hosler, Alexandra M. Huffman, Damian G. Romero, Licy L. Yanes Cardozo
Summary: PCOS model with excess androgen leads to expansion of adipose tissue and affects mitochondrial content and function. SGLT2i reduces adiposity by improving the frequency of small adipocytes in adipose tissue, without affecting mitochondrial function and oxidative stress.
BIOLOGY OF SEX DIFFERENCES
(2022)
Article
Biochemistry & Molecular Biology
Max Baymiller, Benjamin Nordick, Connor M. Forsyth, Susan A. Martinis
Summary: Aminoacyl-tRNA synthetases are ancient and ubiquitous components of all organisms. In this study, a leukocyte-specific exon skipping event in human leucyl-tRNA synthetase (LARS) was discovered using long-read sequencing. The splice variant LSV3, regulated by SRSF1, retains its role as a leucine sensor and signal transducer despite lacking tRNA leucylation activity. Alternative splicing of LARS separates its ancient catalytic activity from its more recent evolutionary role in cell signaling, providing functional specificity in human immune cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Haihong Guo, Nengwei Xu, Malte Prell, Hiltrud Koenigs, Benita Hermanns-Sachweh, Bernhard Luescher, Ferdinand Kappes
Summary: The BGIS method investigates recombinant protein toxicity in E. coli, identifying protein domains and enabling screening. The study discovered an unrecognized RNA-interacting domain in the DEK oncoprotein, and successfully used BGIS for screening loss-of-function mutants in the DNA modulating domain.
Article
Critical Care Medicine
Man Li, Junqiao Lv, Zhiqiang Wang, Liping Wang, Zhixin Qin, Chen Deng, Jinming Liu, Lin Sun
Summary: HMGB1 promotes the expression of NKCC1 and NHE1 in astrocytes and endothelial cells through activation of the HMGB1-TLR4-TRIF-NF-kappa B signaling pathway, leading to cytotoxic and ionic edema following spinal cord injury. Inhibition of HMGB1 reduces the expression of NKCC1 and NHE1 and decreases spinal cord edema. These findings are important for understanding the regulation of spinal cord edema after injury.
JOURNAL OF NEUROTRAUMA
(2023)