Article
Biochemistry & Molecular Biology
German Higuera-Martinez, Ivonne Maciel Arciniega-Martinez, Rosa Adriana Jarillo-Luna, Luz Maria Cardenas-Jaramillo, David Levaro-Loquio, Maritza Velasquez-Torres, Edgar Abarca-Rojano, Aldo Arturo Resendiz-Albor, Judith Pacheco-Yepez
Summary: Amebiasis is an intestinal infection caused by Entamoeba histolytica. Amebic liver abscess (ALA) is the most common extraintestinal complication of amebiasis. Neutrophils play a key role in the initial phase of ALA through reactive oxygen species (ROS) and the enzyme NADPH oxidase (NOX2). Apocynin, a natural inhibitor of NOX, reduces ALA progression by decreasing NOX activity and damaging amoebae in vitro.
Article
Immunology
Marina Nayeli Medina-Rosales, Martin Humberto Munoz-Ortega, Mariana Haydee Garcia-Hernandez, Patricia Talamas-Rohana, Iliana Ernestina Medina-Ramirez, Larissa Guadalupe Salas-Moron, Sandra Luz Martinez-Hernandez, Manuel Enrique Avila-Blanco, Beatriz Medina-Rosales, Javier Ventura-Juarez
Summary: The study found that ACh enhances the pathogenicity of E. histolytica by upregulating various virulence factors, enhancing activities like erythrophagocytosis, cytotoxicity, and actin cytoskeleton remodeling, leading to more severe disease conditions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Maria S. Salazar, Carlos D. Maya, Mario Cervantes, Ajmani Surainder
Summary: Amebiasis is a parasitic infection caused by the protozoan Entamoeba histolytica, commonly seen in migrants and travelers. Liver infection can lead to liver abscesses, and a fraction of patients may develop lung involvement. Diagnosis is based on clinical, imaging, and serology studies. Treatment options include medication and surgical drainage.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)
Review
Medicine, General & Internal
Daisuke Usuda, Shiho Tsuge, Riki Sakurai, Kenji Kawai, Shun Matsubara, Risa Tanaka, Makoto Suzuki, Hayabusa Takano, Shintaro Shimozawa, Yuta Hotchi, Shungo Tokunaga, Ippei Osugi, Risa Katou, Sakurako Ito, Kentaro Mishima, Akihiko Kondo, Keiko Mizuno, Hiroki Takami, Takayuki Komatsu, Jiro Oba, Tomohisa Nomura, Manabu Sugita
Summary: Amebic liver abscesses (ALAs) are a common extraintestinal manifestation of human invasive amebiasis caused by Entamoeba histolytica. ALAs result from the invasion of the intestinal mucosa, and diagnosis requires liver imaging and antibody detection. ALAs can be treated with medication, and the prognosis is generally good.
WORLD JOURNAL OF CLINICAL CASES
(2022)
Article
Immunology
Marisol Pacheco-Sanchez, Sandra Luz Martinez-Hernandez, Martin Humberto Munoz-Ortega, Jesus Alejandro Reyes-Martinez, Manuel Enrique avila-Blanco, Javier Ventura-Juarez
Summary: The aim of this study is to identify a membrane receptor in E. histolytica trophozoites for acetylcholine (ACh). Results show that the Gal/GalNAc lectin acts as a possible binding site for ACh and this binding may occur through the 150 kDa intermediate subunit. At the same time, this interaction activates the GTPases, Ras, and Rab, which are involved in the proliferation, and reorganization of the amoebic cytoskeleton and vesicular trafficking.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Yumiko Saito-Nakano, Takashi Makiuchi, Mami Tochikura, Carol A. Gilchrist, William A. Petri, Tomoyoshi Nozaki
Summary: The protist EhArfX2, involved in vesicular traffic regulation, is highly upregulated in a virulent strain of Entamoeba histolytica. Its function is essential for intracellular transportation and defense against reactive oxygen and nitrogen species, contributing to virulence in the host organism.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Shruti Nagaraja, Maggi W. Cai, Jingjing Sun, Hugo Varet, Lotem Sarid, Meirav Trebicz-Geffen, Yana Shaulov, Mohit Mazumdar, Rachel Legendre, Jean-Yves Coppee, Thomas J. Begley, Peter C. Dedon, Samudrala Gourinath, Nancy Guillen, Yumiko Saito-Nakano, Chikako Shimokawa, Hajime Hisaeda, Serge Ankri
Summary: Queuine has a dual role in promoting oxidative stress resistance and reducing virulence in the eukaryotic parasite Entamoeba histolytica. It is efficiently incorporated into the parasite's tRNAs and stimulates methylation, leading to protection against oxidative stress and downregulation of virulence-associated genes. Silencing of EhTGT prevents queuine incorporation, impairing parasite growth, oxidative stress resistance, and cytopathic activity.
Article
Public, Environmental & Occupational Health
Aradhana Singh, Tuhina Banerjee, Sunit Kumar Shukla
Summary: Recurrence of amebic liver abscess (ALA) is becoming increasingly reported, with factors such as presence of resistance genes, bacterial flora, and abscess size being associated with recurrence.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Infectious Diseases
Andrea L. Maricuto, Viledy L. Velasquez, Jacinto Pineda, David M. Flora-Noda, Isaac Rodriguez, Crismar A. Rodriguez-Ines, Oscar O. Noya-Gonzalez, Rosa Contreras, Oscar D. Omana-Avila, Ivan A. Escalante-Perez, Natasha A. Camejo-Avila, Nicolle A. Kuffaty-Akkou, Fhabian S. Carrion-Nessi, Martin Carballo, Maria E. Landaeta, David A. Forero-Pena
Summary: This study presents a case of a patient with COVID-19 co-presenting with ALA. The case suggests that coinfection with SARS-CoV-2 and Entamoeba histolytica could impact the patient's clinical course, but larger studies are needed to fully understand the interaction between these pathogens.
BMC INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Francisco Sierra-Lopez, Lidia Baylon-Pacheco, Sonia Cynthia Vanegas-Villa, Jose Luis Rosales-Encina
Summary: Entamoeba histolytica, an intestinal protozoan parasite, has two low molecular weight protein tyrosine phosphatase genes, EhLMW-PTP1 and EhLMW-PTP2, with one amino acid difference between them. These genes show different activities and stabilities under various conditions.
Article
Microbiology
Yasuaki Yanagawa, Shinji Izumiyama, Yumiko Saito-Nakano, Kumiko Nakada-Tsukui, Seiki Kobayashi, Naoko Yoshida, Yoshimi Kikuchi, Hiroyuki Gatanaga, Shinichi Oka, Tomoyoshi Nozaki, Koji Watanabe
Summary: The severity of Entamoeba histolytica infection is determined by host immunology, pathogen virulence, and the intestinal environment. Conventional research for assessing pathogen virulence has been mainly performed using laboratory strains, and it is unclear whether this knowledge can be applied to the true pathogenesis. Transcriptomic analysis of clinical and laboratory strains revealed significant differences in gene expression, indicating that the changes caused by liver challenge may not be consistent between clinical and laboratory strains. Environmental stress can also enhance the virulence of the laboratory strain through a shift in gene expression profile.
Article
Microbiology
Janeth Lozano-Mendoza, Fatima Ramirez-Montiel, Angeles Rangel-Serrano, Itzel Paramo-Perez, Claudia Leticia Mendoza-Macias, Faridi Saavedra-Salazar, Bernardo Franco, Nauru Vargas-Maya, Ghulam Jeelani, Yumiko Saito-Nakano, Fernando Anaya-Velazquez, Tomoyoshi Nozaki, Felipe Padilla-Vaca
Summary: This study found that the virulence of Entamoeba histolytica comes from complex interactions between the parasite and the host, involving various amoebic components and host factors. There were no differences in gene expression of classical virulence factors between the nonvirulent UG10 strain and its parental HM-1:IMSS strain. Overexpression of the EhAIG1 gene increased the virulence of UG10. Thus, the EhAIG1 gene represents a novel virulence determinant in E. histolytica.
Article
Parasitology
Itzia Azucena Rangel-Castaneda, Araceli Castillo-Romero, Gloria Leon-Avila, Martin Zermeno-Ruiz, Jose Manuel Hernandez-Hernandez
Summary: The study showed that terfenadine affects the growth and cell viability of Entamoeba histolytica in a time- and dose-dependent manner, with the most significant inhibitory effects observed with 4 mu M at 48 h. Additionally, terfenadine exhibited low toxicity on Caco-2 cells and significantly inhibited adhesion to Caco-2 cells and erythrophagocytic capacity of the parasite. These findings suggest that terfenadine exerts significant effects on the virulence of Entamoeba histolytica and may be effective in the treatment of amoebiasis.
PARASITOLOGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Mitzi Diaz-Hernandez, Rosario Javier-Reyna, Diana Martinez-Valencia, Sarita Montano, Esther Orozco
Summary: By studying the active movement and phagocytosis of the trophozoites of Entamoeba histolytica, researchers have discovered the dynamics of the ESCRT protein interactions during phagocytosis. They identified the proteins forming the E. histolytica ESCRT-II complex and their interactions with other molecules involved in phagocytosis. This study provides valuable insights into the phagocytosis process and the function of related proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Ravikanth Reddy
Summary: Intestinal amoebiasis and its sequelae, such as amoebic liver abscess, are rarely reported in the modern medical era. Atypical presentation of amoebic liver abscess, with high false-positive results on serology in endemic regions, presents major diagnostic challenges in developing countries. The case study discussed illustrates the importance of detailed medical history and serological testing in the diagnosis and treatment of amoebic liver abscess, with successful management evident in the patient's three-month follow-up.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Correction
Multidisciplinary Sciences
Lydia Enith Nava-Rivera, Nadia Denys Betancourt-Martinez, Rodrigo Lozoya-Martinez, Pilar Carranza-Rosales, Nancy Elena Guzman-Delgado, Irma Edith Carranza-Torres, Hector Delgado-Aguirre, Jose Omar Zambrano-Ortiz, Javier Moran-Martinez
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Alfredo Pherez-Farah, Rosa del Carmen Lopez-Sanchez, Luis Mario Villela-Martinez, Rocio Ortiz-Lopez, Brady E. Beltran, Jose Ascencion Hernandez-Hernandez
Summary: Lymphomas are a highly complex and diverse group of hematological neoplasms, with pathogenic mechanisms involving dysregulation of the sphingolipid family. The crucial role of sphingolipids in virtually every cellular process makes studying their impact in cancer essential.
Article
Oncology
Liliana Gomez-Flores-Ramos, Angelica Leticia Barraza-Arellano, Alejandro Mohar, Miguel Trujillo-Martinez, Lizbeth Grimaldo, Rocio Ortiz-Lopez, Victor Trevino
Summary: This study identified breast cancer-associated genes in young, Mexican women through genetic analysis. The results revealed both well-known genes and rarely reported variants in this population. Furthermore, candidate causal genes were successfully assigned to a significant portion of patients, which could contribute to improved diagnosis, treatment, and life expectancy in young women with breast cancer.
Article
Genetics & Heredity
Gerardo J. Alanis-Funes, Saul Lira-Albarran, Jesus Hernandez-Perez, Mario A. Garza-Elizondo, Rocio Ortiz-Lopez, Cesar Elizondo, Augusto Rojas-Martinez, Rocio A. Chavez-Santoscoy, Claudia Rangel-Escareno
Summary: Currently, there is no established genetic basis to differentiate hypermobility spectrum disorders (HSD) from hypermobile Ehlers-Danlos syndrome (hEDS). This study used whole-exome sequencing to identify DNA mutations in patients diagnosed with generalized HSD. Three genes (MUC3A, RHBG, and ZNF717) were found to be mutated in all five patients evaluated. Functional enrichment analysis revealed the extracellular matrix (ECM) structural constituent as the primary overrepresented molecular function. Comparison with other studies supported the hypothesis of two distinct diseases sharing a common genetic background. Five potential biomarkers for HSD were identified.
Article
Plant Sciences
Liliana De La Cruz-Jimenez, Mario Alberto Hernandez-Torres, Imelda N. Monroy-Garcia, Catalina Rivas-Morales, Maria Julia Verde-Star, Vianey Gonzalez-Villasana, Ezequiel Viveros-Valdez
Summary: Seven medicinal plants from Chiapas, Mexico, were analyzed for their medicinal properties, including antibacterial, antioxidant, antihemolytic, and alpha-glucosidase inhibition activities. T. nelsonii exhibited the highest antimicrobial and antihemolytic activities, suggesting its potential as a source of compounds for cancer treatment and blood sugar control.
Article
Biology
L. N. Espinosa, D. Carrillo, L. Gaytan, F. G. Veliz, J. Moran, R. A. Delgado
Summary: This study reports a case of accidental monensin sodium poisoning in a dairy production system in Mexico. The poisoned calves showed various symptoms and pathological changes in multiple organs.
REVISTA BIO CIENCIAS
(2022)
Article
Environmental Sciences
Jose Javier Garcia Salcedo, Taehyun Roh, Lydia Enith Nava Rivera, Nadia Denys Betancourt Martinez, Pilar Carranza Rosales, Maria Francisco San Miguel Salazar, Mario Alberto Rivera Guillen, Luis Benjamin Serrano Gallardo, Maria Sonadora Nino Castaneda, Nacny Elena Guzman Delgado, Jair Millan Orozco, Natalia Ortega Morales, Javier Moran Martinez
Summary: Multiple comorbidities related to arsenic exposure through drinking water continue to be public problems worldwide. This study conducted a comparative analysis of arsenic levels in pregnant women and their neonates in Mexico, and suggested that breastfeeding may reduce arsenic exposure in infants.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Bryan Alejandro Espinosa-Rodriguez, Daniela Trevino-Almaguer, Pilar Carranza-Rosales, Monica Azucena Ramirez-Cabrera, Karla Ramirez-Estrada, Eder Ubaldo Arredondo-Espinoza, Luis Fernando Mendez-Lopez, Isaias Balderas-Renteria
Summary: Metabolic reprogramming in cancer cells by metformin and biguanides may involve the disruption of L-arginine and related compounds, leading to antitumoral effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Sara Garcia-Davis, Atteneri Lopez-Arencibia, Carlos J. Bethencourt-Estrella, Desiree San Nicolas-Hernandez, Ezequiel Viveros-Valdez, Ana R. Diaz-Marrero, Jose J. Fernandez, Jacob Lorenzo-Morales, Jose E. Pinero
Summary: In this study, it was found that five compounds isolated from the red alga Laurencia johnstonii exhibited leishmanicidal activity. Among them, laurequinone showed the strongest activity and was capable of inducing programmed cell death or apoptosis in the parasite. These findings highlight the potential of laurequinone as a novel therapeutic agent against kinetoplastid parasites.
Article
Infectious Diseases
Carmen Molina-Torres, Carlos Pedraza-Rodriguez, Lucio Vera-Cabrera, Jorge Ocampo-Candiani, Catalina Rivas-Morales, Ezequiel Viveros-Valdez
Summary: Tuberculosis (TB) is still a major global health problem, with antibiotic-resistant strains posing the greatest challenge to its treatment. Additionally, the prevalence of non-tuberculous mycobacteria (NTM) in humans has significantly increased in recent years. This study aims to investigate the antimycobacterial effects of extracts and major compounds from Hedeoma drummondii on both clinical isolates of Mycobacterium tuberculosis and NTM strains. The methanolic extract exhibited the best activity against M. tuberculosis, inhibiting 10 out of 12 strains at a concentration < 2500 mu g/mL, while the hexanic extract showed the best activity against NTM, inhibiting 8 out of 10 strains at <= 625 mu g/mL. Furthermore, there was a strong positive correlation between the antimycobacterial activity of pulegone and the hexanic extract against NTM strains, suggesting its potential as a predictive marker for these microorganisms.
Article
Andrology
Nadia Denys Betancourt-Martinez, Pilar Carranza-Rosales, Ezequiel Viveros-Valdez, Nancy Elena Guzman-Delgado, Maritza Argelia Macias-Corral, Irma Edith Carranza-Torres, Lydia Enith Nava-Rivera, Maria Sonadora Nino Castaneda, Javier Moran-Martinez
Summary: H. drummondii has a protective effect against FA-induced sperm genotoxic damage, maintaining the histological structure of the testis, similar to alpha-tocopherol.
Article
Health Care Sciences & Services
Pilar Carranza-Rosales, Daniel Valencia-Mercado, Olga Esquivel-Hernandez, Manuel Ismael Gonzalez-Geroniz, Jose Inocente Banuelos-Garcia, Ana Lilia Castruita-Avila, Mario Alberto Sanchez-Prieto, Ezequiel Viveros-Valdez, Javier Moran-Martinez, Isaias Balderas-Renteria, Nancy Elena Guzman-Delgado, Irma Edith Carranza-Torres
Summary: Breast cancer is a major cause of death worldwide, and there is growing interest in personalized therapy. This study used organotypic culture of human breast tumor slices to predict the effects of antineoplastics on tumor viability.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Juanita Deniss Perales-Flores, Maria Julia Verde-Star, Jose Ezequiel Viveros Valdez, Maria Porfiria Barron-Gonzalez, Ruth Amelia Garza-Padron, Victor E. Aguirre-Arzola, Ramon Gerardo Rodriguez
Summary: Mexico is one of the main public health problems of Helicobacter pylori, this study evaluated the in vitro activity of walnut and rosemary as well as rue extracts against H. pylori, showing their potential as alternative sources for new treatments.