Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Pilar Escribano, Jesus Guinea, Patricia Munoz, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The implementation of commercial kits for standardized fungal sample preparation and updated reference libraries enables the easy use of MALDI-TOF MS for routine identification of fungal species. Although further improvement in the databases is needed, this method allows for the correct identification of a significant number of fungal species at the species level.
Article
Biochemical Research Methods
Ari Chae, Gwanwon Lee, Dong-Yeun Koh, Cheol-Min Yang, Sungho Lee, Young-Kwan Kim
Summary: Carbon fiber (CNF), prepared from electrospun PAN fibers, shows high salt tolerance, sensitivity, and resolution for matrix-free LDI-MS analysis, making it a promising material for developing an efficient and universal platform for analyte analysis regardless of ionization modes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
John P. McGee, Pei Su, Kenneth R. Durbin, Michael A. R. Hollas, Nicholas W. Bateman, G. Larry Maxwell, Thomas P. Conrads, Ryan T. Fellers, Rafael D. Melani, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Summary: This study introduces a semi-automated single-ion mass spectrometry workflow for top-down tandem mass spectrometry analysis directly from tissue microenvironments. The results demonstrate that this method can identify multiple proteoforms in tumor samples within a short period of time, and effectively identify proteoform signatures in tumor and stromal regions.
NATURE COMMUNICATIONS
(2023)
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Review
Chemistry, Analytical
Yuanyuan Wu, Wenmei Zhang, Yaoyao Zhao, Xiayan Wang, Guangsheng Guo
Summary: Research on single-cell proteomics is crucial for understanding the growth, development, and diseases in organisms. However, the complexity and limited quantity of proteins in single cells have hindered the progress in this field. Mass spectrometry-based single-cell proteomics, particularly electrospray ionization mass spectrometry (ESI-MS), has rapidly advanced due to its minimal sample requirements, high sensitivity, and structural information without antibody labeling. Nevertheless, the variations in pretreatment methods, separation techniques, and MS instruments greatly affect the throughput and coverage of single-cell proteomic technologies. This review categorizes recent publications based on different methods for single-cell protein sample separation, highlights the performance of these methods in terms of detection coverage and throughput, and discusses the applications and future prospects of ESI-MS-based single-cell proteomics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Meletia Kallianta, Eftychia Pappa, Heleni Vastardis, Christos Rahiotis
Summary: Mass Spectrometry (MS) is a highly sensitive and universal analytical technique that has rapidly developed in recent decades. It has various applications in dentistry, including research on dental materials and agents, laboratory analysis of biospecimens, and real-time diagnostics in oral surgery and pathology. MS can provide valuable insights into the clinical behavior, toxicity, antimicrobial properties, and biomarkers of dental materials and agents. It is also effective in detecting biomarkers in saliva and providing a comprehensive understanding of cellular functions through metabolite analysis. Additionally, MS shows promising potential for surgical approaches to oral malignant disorders. Overall, MS has revolutionized dental research and has the potential to advance personalized dentistry.
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Article
Chemistry, Physical
Stefania Bergamini, Elisa Bellei, Luigi Generali, Aldo Tomasi, Carlo Bertoldi
Summary: This study evaluated proteomic changes induced by CHX and CHX+ADS in supragingival dental sites susceptible to discoloration effects. The results showed that long-term use of CHX led to changes in the TSCM proteome, but these changes were reduced when using CHX+ADS. The ADS limited TSCM formation and the antiseptic effect of CHX reduced its impact on bacterial cell permeability, while Maillard's reaction produced molecules that could facilitate bacterial adhesion.
Article
Chemistry, Analytical
Ying Feng, Xianhu Wei, Moutong Chen, Honghui Zhu, Jumei Zhang, Youxiong Zhang, Liang Xue, Lanyan Huang, Guoyang Chen, Qihui Gu, Shi Wu, Yu Ding, Qingping Wu
Summary: This study developed a new deep semi-quantitative fingerprinting method using pseudotargeted metabolomics and deep learning techniques, achieving a prediction accuracy of Listeria monocytogenes serotypes exceeding 99%. This technology demonstrates great potential for the rapid and accurate identification of pathogens.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
