Journal
EXPERIMENTAL PARASITOLOGY
Volume 127, Issue 1, Pages 215-221Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.07.020
Keywords
Leishmania; Yangambin; Ocotea duckei; Cell death; Confocal microscopy; Transmission electron microscopy
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Funding
- CAPES
- CNPq
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We have previously demonstrated that yangambin, a lignan obtained from Ocotea duckei Vattimo (Lauraceae), shows antileishmanial activity against promastigote forms of Leishmania chagasi and Leishmania amazonensis. The aim of this study was to determine the in vitro effects of yangambin against these parasites using electron and confocal microscopy. L chagasi and L amazonensis promastigotes were incubated respectively with 50 mu g/mL and 65 mu g/mL of pure yangambin and stained with acridine orange. Treated-parasites showed significant alterations in fluorescence emission pattern and cell morphology when compared with control cells, including the appearance of abnormal round-shaped cells, loss of cell motility, nuclear pyknosis, cytoplasm acidification and increased number of acidic vesicular organelles (AVOs), suggesting important physiological changes. Ultrastructural analysis of treated-promatigotes showed characteristics of cell death by apoptosis as well as by autophagy. The presence of parasites exhibiting multiples nuclei suggests that yangambin may also affect the microtubule dynamic in both Leishmania species. Taken together our results show that yangambin is a promissing agent against Leishmania. (C) 2010 Elsevier Inc. All rights reserved.
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