Journal
EXPERIMENTAL PARASITOLOGY
Volume 129, Issue 1, Pages 11-16Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2011.06.003
Keywords
Schistosoma mansoni; Biomphalaria glabrata; Intramolluscan stage; DNA/RNA extraction
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Funding
- French National Agency for Research [ANR-07-BLAN-0214-03]
- CNRS
- UPVD
- Agence Nationale de la Recherche (ANR) [ANR-07-BLAN-0214] Funding Source: Agence Nationale de la Recherche (ANR)
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Detailed studies of host/parasite interactions are currently limited because in situ gene sequencing or monitoring of parasite gene expression is so far limited to genes presenting a high loci copy number in the Schistosome genome or a high level of expression. Indeed, how to investigate the host parasite molecular interplay when parasites are not directly accessible in vivo? Here we describe a method to circumvent this problem and to analyze DNA and RNA of Schistosoma mansoni during the interaction with its intermediate snail host Biomphalaria glabrata. We propose a technique for improved DNA and RNA extraction from the intra-molluscan stage of the parasite recovered after fixation of infected snails in Raillet-Henry solution. The extractions can be used for genetic analysis, transcription studies and microsatellite genotyping. (C) 2011 Elsevier Inc. All rights reserved.
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