Article
Microbiology
Doaa Naguib, Dawn M. Roellig, Nagah Arafat, Lihua Xiao
Summary: This study investigated Cryptosporidium spp. in rabbits from different farms in Egypt, finding an overall detection rate of approximately 11.9%. All 28 samples tested positive for Cryptosporidium cuniculus, with 16 samples successfully subtyped into two subtypes. These findings suggest that Cryptosporidium spp. in rabbits from Egypt have zoonotic potential.
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: The study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan using simple microscopy and PCR techniques. Results showed age and sex were important factors affecting prevalence, with molecular detection being more efficient and sensitive compared to simple microscopy.
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: This study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan, comparing the sensitivity of two diagnostic techniques. The research concluded that molecular detection through PCR is more efficient, specific, and sensitive than simple microscopy for detecting Cryptosporidium infection.
Article
Biotechnology & Applied Microbiology
Samart Dorn-In, Sirkka Mang, Karin Schwaiger
Summary: This study investigated cold-tolerant Clostridium spp. strains isolated from various samples, identifying them into different groups based on genetic and biochemical characteristics. Spiked beef samples showed changes in odor after storage, while maintaining similar color and meat quality compared to the control group.
Article
Agriculture, Dairy & Animal Science
Hyo-Jin Paek, Zhou-Yan Li, Biao-Hu Quan, Xi-Jun Yin
Summary: Knockout of the MSTN gene is associated with enlarged tongue and suckling difficulty in animals, leading to high mortality. This study developed a PCR-RFLP genotyping method for MSTN mutant pig breeding, providing a simple and fast way to determine the genotype of pigs and contribute to commercial breeding systems and production of double muscle pigs.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Mercedes Fernandez-Escobar, Federica Giorda, Virgina Mattioda, Tania Audino, Fabio Di Nocera, Giuseppe Lucifora, Katia Varello, Carla Grattarola, Luis Miguel Ortega-Mora, Cristina Casalone, Rafael Calero-Bernal
Summary: This study investigated the genetic diversity of Toxoplasma gondii strains in stranded dolphins found in the Mediterranean coastlines of Italy. The results showed that dolphins were mainly infected with ToxoDB#3 and #2 genotypes.
Article
Biochemical Research Methods
Anshuman Kumar, Ishwar Dayal Gupta, Govind Mohan, M. R. Vineeth, D. Ravi kumar, S. Jayakumar, Ranjit Singh Kataria, Saket Kumar Niranjan
Summary: Two PCR-based assays using T-ARMS-PCR and PCR-RFLP were developed to screen for the JH1 mutation in Jersey cattle, leading to the identification of carriers in Indian Jersey bulls for the first time. These PCR assays are cost-effective, rapid, and accurate, and can be used separately or in combination for screening and cross-validation.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Veterinary Sciences
Jacqueline King, Anne Pohlmann, Kamila Dziadek, Martin Beer, Kerstin Wernike
Summary: Bovine viral diarrhea virus (BVDV) is a significant pathogen causing economic losses globally. The genetic variability of BVDV highlights the importance of whole-genome sequencing for identifying novel strains and supplementing missing sequence information in public repositories. The proposed amplicon-based sequencing protocol allows rapid, inexpensive, and accessible obtainment of complete BVDV genomes.
BMC VETERINARY RESEARCH
(2021)
Article
Parasitology
N. P. Mthethwa, I. D. Amoah, P. Reddy, F. Bux, S. Kumari
Summary: This study developed and validated a molecular protocol for DNA extraction, efficient recovery of protozoan parasites, and quantification in wastewater using droplet digital polymerase chain reaction (ddPCR). The conventional phenol-chloroform extraction method showed the highest DNA concentration and detection of Cryptosporidium parvum compared to other methods. The optimized protocol successfully measured the protozoan concentration in wastewater from different regions in South Africa and could be effective in monitoring and mitigating water-borne infections.
EXPERIMENTAL PARASITOLOGY
(2022)
Article
Parasitology
M. A. Mohamed, H. M. Hammam, H. A. El-Taweel, N. F. Abd El-latif
Summary: This study investigated the species of Cryptosporidium in HIV patients in Egypt and their associated features. The results showed that Cryptosporidium infection is common and symptomatic in HIV patients in Egypt. The dominant species, C. hominis, C. parvum, and C. meleagridis, have distinct distributions in urban and rural residents.
TROPICAL BIOMEDICINE
(2022)
Article
Food Science & Technology
Zoe Gounari, Stamatoula Bonatsou, Ilario Ferrocino, Luca Cocolin, Olga S. Papadopoulou, Efstathios Z. Panagou
Summary: This study investigated the physicochemical characteristics and microbiological composition of naturally black dry-salted olives obtained from different retail outlets in the Greek market. The results showed significant variability in the physicochemical attributes among the samples, with pH values ranging from 4.0 to 5.0 and water activity values ranging from 0.58 to 0.91. The dominant yeast species identified were Pichia membranifaciens, Candida sorbosivorans, Citeromyces nyon-sensis, Candida etchelsii, Wickerhamomyces subpelliculosus, Candida apicola, Wickerhamomyces anomalus, Torulaspora delbrueckii, and Candida versatilis. The majority of the samples met the microbiological and hygienic quality requirements, but there was a lack of standardization in the processing of this commercial style.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Entomology
Alenka Zugelj, Bojan Papic, Irena Zdovc, Urska Zajc, Majda Golob, Jana Avbersek, Darja Kusar
Summary: The study investigated the distribution of ERIC types of Paenibacillus larvae in Slovenia, where ERIC II was found to be the predominant type at 70.2%. Furthermore, whole-genome sequencing of outbreak-related ERIC I isolates revealed seven outbreak clusters with transmission occurring within a 3-km radius and between geographically distant apiaries, emphasizing the role of beekeeping activities in the transmission of P. larvae.
Article
Clinical Neurology
Raziyeh Khalesi, Ehsan Razmara, Golareh Asgaritarghi, Ali Reza Tavasoli, Yasser Riazalhosseini, Daniel Auld, Masoud Garshasbi
Summary: This study identified potential genetic factors causing Warburg micro syndrome in two Iranian patients, validated candidate variants through Sanger sequencing, and demonstrated novel manifestations associated with the disease, expanding the genotype-phenotype spectrum of WARBM type 1.
Article
Plant Sciences
Harleen Kaur, Jhulia Gelain, Marcus Vinicius Marin, Natalia A. Peres, Guido Schnabel
Summary: A new Neopestalotiopsis sp. has been discovered in strawberry plants in Florida, Georgia, and South Carolina, causing severe leaf spot and fruit rot. This new species is more aggressive and destructive than other Pestalotiopsis pathogens, leading to significant yield losses for affected growers. The study developed a molecular method based on PCR/RFLP for accurate and fast identification of the new Neopestalotiopsis sp. variant from strawberry plants. This method utilizes PCR amplification of a beta-tubulin gene fragment and the restriction enzyme BsaWI to differentiate the new species from others.
Article
Microbiology
Laure Claudel, Nicolas Valeix, Louise Basmaciyan, Bruno Pereira, Damien Costa, Anne Vincent, Stephane Valot, Loic Favennec, Frederic Dalle
Summary: The study evaluated 11 commercial mechanical pretreatment matrixes for their performances in improving Cryptosporidium parvum oocyst DNA extraction, finding that the performance varied depending on the composition, size, and shape of the beads.
