Journal
EXPERIMENTAL PARASITOLOGY
Volume 118, Issue 4, Pages 536-542Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2007.11.012
Keywords
HSP70; fasciola hepatica; fasciola gigantica; fasciolosis; liver fluke; parasite; trematode; ESP; excretory-secretory products; HSP; heat-shock protein; ITT; Indonesian thin tail; rFhHSP70; recombinant F hepatica 70 kDa heat-shock protein; WWL; whole worm lysate
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Fasciola hepatica and Fasciola gigantica are trematode parasites responsible for fasciolosis, a disease of ruminant animals which is also increasingly recognised as a disease in humans. By biochemical and in silico methods, we have cloned and characterised the 70 kDa heat-shock proteins (HSP70s) of F hepatica and F gigantica. The nucleotide and protein sequences for HSP70 were found to be 98% and 99% identical between liver fluke species, respectively, and to encode conserved amino acid motifs that are of putative functional importance. Western blot analysis demonstrated that HSP70 proteins were expressed at a higher level in F gigantica recovered from sheep relative to F hepatica, but HSP70 was not detected in the excretory-secretory products of these liver fluke samples. Real-time reverse-transcriptase PCR analysis of HSP70 expression in parasites from sheep, but not cattle, showed HSP70 expression to be higher in F gigantica than F hepatica. These results suggest that hosts refractory to F gigantica are associated with higher HSP70 expression by this parasite and that HSP70 expression may represent a biochemical marker of the stress response of F gigantica. (c) 2007 Elsevier Inc. All rights reserved.
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