Article
Microbiology
Harrison Dulin, Nathan Hendricks, Duo Xu, Linfeng Gao, Keidy Wuang, Huiwang Ai, Rong Hai
Summary: Influenza virus proteins can be nitrated, which decreases virus infectivity and replication ex vivo, but the inhibitory effect of nitration on virus functions in vivo may be insufficient. Understanding the role of peroxynitrite during influenza virus infections can provide valuable insights.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Athanasios Fysikopoulos, Michael Seimetz, Stefan Hadzic, Fenja Knoepp, Cheng-Yu Wu, Kathrin Malkmus, Jochen Wilhelm, Alexandra Pichl, Mariola Bednorz, Elsa Tadele Roxlau, Hossein A. Ghofrani, Natascha Sommer, Mareike Gierhardt, Ralph T. Schermuly, Werner Seeger, Friedrich Grimminger, Norbert Weissmann, Simone Kraut
Summary: The study demonstrates that inhibiting iNOS can potentially promote lung regeneration, improve emphysema, and reverse pulmonary hypertension in a mouse model of severe emphysema without tobacco smoke exposure.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Plant Sciences
Magdalena Arasimowicz-Jelonek, Przemyslaw Jagodzik, Artur Plociennik, Ewa Sobieszczuk-Nowicka, Autar Mattoo, Wladyslaw Polcyn, Jolanta Floryszak-Wieczorek
Summary: Peroxynitrite plays a crucial role in regulating the nitro-oxidative environment during dark-induced leaf senescence in Arabidopsis. It selectively modifies bio-targets including proteins and nucleic acids. The formation of peroxynitrite coincides with the decrease in nitric oxide emission during leaf senescence. Different amino acids and nucleotides in proteins and nucleic acids are nitration targets of peroxynitrite, and this modification has a significant impact on protein biosynthesis and carbohydrate metabolism.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Cell Biology
Wan-Chao Yang, Hong-Ling Cao, Yue-Zhen Wang, Ting-Ting Li, Hong-Yu Hu, Qiang Wan, Wen-Zhi Li
Summary: Hyperglycemia worsens brain damage by affecting vascular endothelial function. Nitric oxide synthase plays a crucial role in diabetic rats with traumatic brain injury, including anti-inflammatory, anti-oxidative stress, and anti-apoptotic activities.
NEURAL REGENERATION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Shikai Liu, Wenting Li, Hengxing Chen, Jialing Zhou, Shuming Dong, Pengyu Zang, Boshi Tian, He Ding, Shili Gai, Piaoping Yang, Yanli Zhao
Summary: This study presents a two-dimensional nanoplatform that integrates a NO donor-modified LiYF4:Ce scintillator and graphitic carbon nitride nanosheets to generate highly cytotoxic peroxynitrite (ONOO-) on demand. The ONOO- effectively enhances the therapeutic efficacy of radiotherapy by inducing mitochondrial and DNA damage and inhibiting glutamine synthetase (GS) to delay DNA damage repair, overcoming the limitations of clinical radiotherapy.
Article
Chemistry, Multidisciplinary
Yongyan Deng, Yupeng Wang, Fan Jia, Weifeng Liu, Dongfang Zhou, Qiao Jin, Jian Ji
Summary: This study reports a novel supramolecular prodrug nanoassemblies strategy that co-delivers nitric oxide and superoxide anion inducing chemotherapeutic agents for enhanced reactive nitrogen species effects in chemotherapy. The efficacy of this strategy was validated in hepatoma models, providing a new direction for cancer treatment.
Article
Materials Science, Biomaterials
Ping Huang, Zihong Li, Li Nong, Jie Cheng, Weiying Lin
Summary: Aging is a natural process characterized by the decline of physiological functions in the body's organs and tissues. Oxidative stress, caused by active nitrogen substances like ONOO-, is considered to be the main cause of aging. Detecting changes in ONOO- in aging cells is important for studying senescence. In this study, we developed a fluorescent probe FLASN based on flavonol that can detect and treat ONOO- in cells. Our results showed that FLASN can effectively detect ONOO- in cells and reduce its production stimulated by metformin. This study provides a new perspective on the role of ONOO- in senescence and the potential therapeutic effect of flavonol.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Plant Sciences
Neha Gupta, Arun Kumar Mishra
Summary: NOS-derived NO plays a critical role in protecting cyanobacteria from oxidative burst and maintaining their physiological balance. Addition of L-NAME inhibitor reduces NO accumulation, while addition of L-arginine increases NO synthesis. Inhibition of NOS leads to decreased growth and metabolic activity of cyanobacteria, as well as decline in photopigments, PSII efficiency, loss in membrane integrity and DNA damage, ultimately resulting in cell death.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Stanislava Vrankova, Zuzana Galandakova, Jakub Benko, Martina Cebova, Igor Riecansky, Olga Pechanova
Summary: The results of the study indicate that 10 weeks of social isolation led to a significant decrease in PPI, accompanied by a decrease in NOS activity in the cerebral cortex and the cerebellum, an increase in iNOS in the hippocampus, and an increase in CD concentration in cortex homogenate. On the other hand, 29 weeks of social isolation had an opposite effect on NOS activity, with an increase in the cerebral cortex and the cerebellum in animals reared in a social environment, accompanied by a decrease in CD concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Seyhan Taskin, Hakim Celik, Gokhan Cakirca, Vildan Manav, Abdullah Taskin
Summary: This study found that patients with alopecia areata are exposed to potent nitrosative stress. Specifically, peroxynitrite, acting as a bridge between reactive oxygen species and reactive nitrogen species, triggers the expansion of oxidative stress cascade. Nitrosative stress may play a role in the pathogenesis of alopecia areata. Nitrosative stress parameters, particularly NOS activity, may be potential markers for alopecia areata.
JOURNAL OF COSMETIC DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Birandra K. Sinha, Erik J. Tokar, Carl D. Bortner
Summary: NCX4040 exhibits cytotoxicity against ovarian tumor cells and induces apoptosis and oxidative stress. Sensitivity to NCX4040 and DNA damage repair efficiency vary among different cell lines, and the cytotoxicity may be mediated by peroxynitrite.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Xiang-Sheng Zhang, Yue Lu, Wen Li, Tao Tao, Lei Peng, Wei-Han Wang, Sen Gao, Cang Liu, Zong Zhuang, Da-Yong Xia, Chun-Hua Hang, Wei Li
Summary: The study showed that astaxanthin significantly reduced oxidative stress and neuronal apoptosis after traumatic brain injury. Astaxanthin improved neurological functions through the SIRT1/Nrf2/Prx2/ASK1/p38 signaling pathway, suggesting it as a potential candidate for alleviating traumatic brain injury.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Lei Wen, Xinyu Ma, Jing Yang, Minmin Jiang, Chao Peng, Zhongyun Ma, Huan Yu, Yinhui Li
Summary: This study proposed a new design strategy based on pi-conjugation modulation for fluorescent probes, which can generate different emission bands in the presence and absence of the target. The newly designed fluorescence probe, Rhod-DCM-B, exhibited outstanding sensitivity and excellent selectivity towards ONOO-. It was successfully employed to determine endogenous and exogenous ONOO- in living cells, and effectively monitored the ONOO- in vivo.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yujin L. Kim, Joshua T. Plank, Bo Li, Alexander R. Lippert
Summary: This article presents a kinetics-based fluorescence method for quantitative tracking of peroxynitrite generation from donor compounds, providing a new approach for real-time quantitative monitoring of peroxynitrite.
ANALYTICAL CHEMISTRY
(2022)
Article
Ophthalmology
Tadanobu Yoshikawa, Kenji Obayashi, Kimie Miyata, Keigo Saeki, Nahoko Ogata
Summary: The study revealed a significant association between higher serum ADMA levels and severe glaucoma, which remained significant after adjusting for potential confounders.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Neurosciences
Wenzhu Wang, Zihan Li, Yitong Yan, Shuo Wu, Xinyu Yao, Chen Gao, Lanxiang Liu, Yan Yu
Summary: This study investigated the reparative mechanisms of low-intensity pulsed ultrasound (LIPUS) in traumatic brain injury (TBI) and found that LIPUS promotes hippocampal neurogenesis, enhances neural electrical activity and neural plasticity, ultimately restoring neuronal function and cognitive capabilities in TBI mice.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Wenmin Yi, Fei Chen, Minghao Yuan, Chuanling Wang, Shengyuan Wang, Jie Wen, Qian Zou, Yinshuang Pu, Zhiyou Cai
Summary: The study suggests that a high-fat diet may lead to tau hyperphosphorylation and synaptic dysfunction by inhibiting the SIRT1/AMPK pathway and disrupting autophagy flux, ultimately resulting in cognitive decline.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Kim M. Hemsley, Helen Beard, Glyn Chidlow, Teresa Mammone, Leanne K. Winner, Daniel Neumann, Barbara King, Marten F. Snel, Paul J. Trim, Robert J. Casson
Summary: Optical coherence tomography (OCT) is a non-invasive method that can be used to rapidly and quantitatively examine the integrity of the neuroretina. It has been shown that OCT can be used to observe retinal thinning in patients with childhood dementia, and to assess the improvement of retinal structure after treatment. Furthermore, OCT can provide insights into other childhood dementias based on the correlation between retinal and brain degeneration in Sanfilippo syndrome.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Qianling Jiang, Xin Ma, Gaochen Zhu, Wen Si, Lingyu He, Guan Yang
Summary: This study investigated the effects of EAE induction on thymopoiesis and T cell development, revealing changes such as increased apoptosis, decreased proliferation, and a blockade in the transition from double-negative thymocytes to double-positive cells. It was also found that positive selection was disrupted in the thymus of EAE mice, along with an increased production of regulatory T cells.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Alice McDouall, Guido Wassink, Sumudu Ranasinghe, Kelly Q. Zhou, Rashika N. Karunasinghe, Justin M. Dean, Joanne O. Davidson
Summary: This study found that blocking connexin 43 hemichannels can attenuate brain injury and promote neurodevelopment in infants with mild hypoxic-ischemic encephalopathy, without causing hypothermia.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Hannah Sweetman, Mahmudur Rahman, Aditya Vedantam, Kajana Satkunendrarajah
Summary: Degenerative cervical myelopathy (DCM) is a neurological condition characterized by chronic compression of the cervical spinal cord, leading to impaired limb function. While respiratory dysfunction is not a common symptom of DCM, it can affect the ventilatory response to respiratory challenges. Surgical decompression improves sensorimotor function in DCM, but its impact on respiratory function is unclear. This study evaluates respiratory function and adaptive ventilation in a DCM model, showing that DCM impairs acute adaptive ventilatory ability and surgical decompression does not fully restore it.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Chengmei Sun, Muhammad Saif Ur Rahman, Budbazar Enkhjargal, Jianhua Peng, Keren Zhou, Zhiyi Xie, Lingyun Wu, Tongyu Zhang, Qiquan Zhu, Jiping Tang, Yujia Zeng, John H. Zhang, Shanshan Xu
Summary: This study found that Osteopontin (OPN) can attenuate inflammatory responses after subarachnoid hemorrhage (SAH) by promoting an anti-inflammatory microglial state. This effect may be mediated through the integrin-FAK-STAT3 and NF-kappa B signaling pathways.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Yang Yang, Xuezhu Chen, Chuanyan Yang, Mei Liu, Qianying Huang, Likun Yang, Yuhai Wang, Hua Feng, Zhongyang Gao, Tunan Chen
Summary: The study explores the effects of specific chemogenetic stimulation of intact corticospinal tract on functional recovery after stroke in mice. The findings demonstrate that combining chemogenetic activation with rehabilitation training leads to significant motor functional recovery by promoting axon sprouting and rewiring new functional circuits.
EXPERIMENTAL NEUROLOGY
(2024)