Article
Cell Biology
Yuki Yoshimoto, Akiyoshi Uezumi, Madoka Ikemoto-Uezumi, Kaori Tanaka, Xinyi Yu, Tamaki Kurosawa, Shinsei Yambe, Kazumitsu Maehara, Yasuyuki Ohkawa, Yusuke Sotomaru, Chisa Shukunami
Summary: This study developed a method using induced pluripotent stem cells (iPSCs) to induce mature tenocytes expressing tenomodulin by monitoring green fluorescent protein (GFP) expression. Single-cell RNA sequencing revealed distinct clusters of tenocytes and a differentiation trajectory, and highlighted the importance of retinoic acid (RA) as a regulatory pathway for tenogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Anatomy & Morphology
Amirah Zulkifli, Raja Elina Ahmad, Shaliny Krishnan, Peggy Kong, Hui Yin Nam, Tunku Kamarul
Summary: Tendon injuries are difficult to treat due to the limited repair ability of tendon tissues. Mesenchymal stem cells (MSC) therapy shows promise in enhancing tendon repair, especially under hypoxic conditions. This review focuses on the role of hypoxia-inducible factor-1-alpha (HIF-1 alpha) in mediating MSC tenogenic differentiation pathways and discusses the interaction between HIF-1 alpha and fibroblast growth factor-2 (FGF-2) and transforming growth factor-beta 1 (TGF beta 1). Strategies to stabilize HIF-1 alpha can improve the efficacy of MSC therapy for tendon repair.
Article
Cell & Tissue Engineering
Guanyin Chen, Dong Fan, Wangqian Zhang, Shuning Wang, Jintao Gu, Yuan Gao, Lei He, Weina Li, Cun Zhang, Meng Li, Yingqi Zhang, Zhaohui Liu, Qiang Hao
Summary: This study found that under hypoxic conditions, there was a significant increase in the expression of Mkx in MSCs. Mkx mediated hypoxia-induced tenogenic differentiation of MSCs but could not completely repress the proliferation of hypoxic MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Hong Cheng, Yan Huang, Wei Chen, Jifei Che, Taidong Liu, Jing Na, Ruojin Wang, Yubo Fan
Summary: The combinational effect of cyclic strain and electrical stimulation was found to significantly promote the neural differentiation of rat bone marrow-derived mesenchymal stem cells, enhancing the expression of neural markers and upregulating neurotrophic factors and cAMP. In addition, co-stimulation further enhanced calcium influx and phosphorylation of ERK1/2 and AKT in neural differentiated BMSCs, suggesting a synergistic effect of the combination on stem cell neuronal differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Health Care Sciences & Services
Aigul R. Rakhmatullina, Rimma N. Mingaleeva, Dina U. Gafurbaeva, Olesya N. Glazunova, Aisylu R. Sagdeeva, Emil R. Bulatov, Albert A. Rizvanov, Regina R. Miftakhova
Summary: This study successfully immortalized adipose-derived MSCs by overexpressing hTERT gene and knocking down TP53, maintaining the differentiation potential and cell morphology stability during long-term culture.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell & Tissue Engineering
Bing-Yu Zhang, Pu Xu, Qing Luo, Guan-Bin Song
Summary: By adding TGF-beta 1 in a collagen sponge-based 3D culture system, the proliferation and tenogenic differentiation of rat BMSCs can be enhanced. The 3D culture significantly improved the differentiation of BMSCs into tenocytes compared to 2D culture, with TGF-beta 1 playing a crucial role in this process.
WORLD JOURNAL OF STEM CELLS
(2021)
Article
Engineering, Biomedical
Hyeree Park, Showan N. Nazhat, Derek H. Rosenzweig
Summary: In this study, a new gel aspiration-ejection method was used to fabricate aligned dense collagen hydrogel scaffolds, which showed regenerative potential for short-term mechanoactivated tenogenic differentiation without the need for serum and growth factors.
Article
Biochemistry & Molecular Biology
Xin Zhou, Junhong Li, Antonios Giannopoulos, Paul J. Kingham, Ludvig J. Backman
Summary: The secretome from myoblasts affects tenocyte migration, cell phenotype, and collagen production, with statically loaded myoblasts' secretome showing a more significant effect on enhancing tenocyte migration and collagen production. Statically loaded myoblasts' secretome also induces a myofibroblast-like phenotype in tenocytes, demonstrating a profound influence on parameters related to tendon healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Hui Yin Nam, Mohd Rusdi Yusof, Tunku Kamarul
Summary: The study aimed to determine the superior tenogenic expressions of adipose derived mesenchymal stromal cells (AD-MSCs) and bone marrow derived-MSCs (BM-MSCs) when subjected to cyclical tensile loading. The results showed that both cell types demonstrated similar tenogenic phenotypic and gene expression levels in a similar loading environment.
STEM CELLS INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Agnieszka Zok, Mariusz Kaczmarek, Wojciech Pienkowski, Paul Mozdziak, Hanna Piotrowska-Kempisty, Bartosz Kempisty
Summary: Wharton's jelly (WJ) from umbilical cords contains mesenchymal stem cells (MSCs) with immunomodulatory properties and differentiation capacity, which makes them a promising tool for cellular therapies. In this study, WJ-MSCs were cultured for differentiation into osteogenic, chondrogenic, adipogenic, and neurogenic lineages, and differentially expressed genes were identified using RNA analysis. The study provides insights into the transcriptomic changes and identification of novel markers in the differentiation process of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Wojciech Pienkowski, Marcin Rucinski, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okolow, Piotr Dziegiel, Bartosz Kempisty
Summary: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) possess multilineage differentiation potential and specific surface markers. In this study, RNA sequencing analysis revealed differentially expressed genes associated with apoptosis and identified upregulation of ZBTB16 and FOXO1, as well as downregulation of TGFA, in all differentiated groups. Additionally, several novel marker genes related to WJ-MSCs differentiation were identified. This study sheds light on the molecular mechanisms involved in long-term culture and differentiation of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Jianyun Liu, Shan He, Baicheng Ma, Xingnuan Li, Yaqin Wang, Jianjun Xiong
Summary: This study used quantitative proteomic analysis to screen for protein expression profiles involved in early-stage osteogenesis and adipogenesis of MSCs. Two upregulated proteins, FBLN2 and NPR3, were identified to participate in the osteogenic differentiation process. The proteomics analysis also revealed functional alterations associated with FBLN2 and NPR3 deficiency.
Article
Materials Science, Biomaterials
Zhuoxin Chen, Hong Xiao, Hongbo Zhang, Qiangwei Xin, Haochen Zhang, Haixin Liu, Mingzhen Wu, Liangrui Zuo, Jun Luo, Qiang Guo, Chunmei Ding, Hong Tan, Jianshu Li
Summary: A novel heterogeneous bilayer hydrogel scaffold was fabricated, mimicking the extracellular matrix of natural cartilage and subchondral bone, to promote the repair of cartilage and bone defects. The incorporation of GelMA, AGA, VPA, and calcium ions showed promising results in promoting chondrogenesis and osteogenesis, as confirmed by in vivo evaluation. This cost-effective and efficient cell-free hydrogel scaffold has great potential for osteochondral repair and inspires the development of tissue-engineering scaffolds.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Cell Biology
Elahe Karimi, Negin Vahedi, Reza Ramezani Sarbandi, Azim Parandakh, Camellia Ganjoury, Faraz Sigaroodi, Najmeh Najmoddin, Mohammad Tabatabaei, Mohammad Tafazzoli-shadpour, Abdolreza Ardeshirylajimi, Mohammad-Mehdi Khani
Summary: By utilizing nanovibration, we were able to direct the differentiation of mesenchymal stem cells (MSCs) into tendon-specific cells without the use of chemical growth factors or complex scaffolds. This finding has significant implications for mechanoregulation of MSCs in stem cell engineering and regenerative medicine applications.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2023)
Article
Biochemistry & Molecular Biology
Honorata Kraskiewicz, Piotr Hinc, Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Maria Paprocka, Danuta Witkowska, Isma Liza Mohd Isa, Abhay Pandit, Aleksandra Klimczak
Summary: A potential treatment for chronic wounds using a hydrogel loaded with secretome from adipose tissue mesenchymal stem cells has been proposed. The biocompatibility and pro-angiogenic activity of the hydrogel-released factors were investigated, showing positive effects on skin-origin cell proliferation and endothelial cell angiogenic properties. Additionally, antimicrobial activity was observed in the secretome, indicating its potential use as an advanced wound-healing dressing in combination with the hydrogel.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Qiuping Liu, Jinghui Sun, Qing Luo, Yang Ju, Guanbin Song
Summary: Salinomycin suppresses the stemness properties of LCSCs by inhibiting the Wnt/beta-catenin signaling pathway and inducing differentiation. This study suggests that salinomycin may serve as a potential drug for liver cancer therapy targeting LCSCs.
CURRENT STEM CELL RESEARCH & THERAPY
(2021)
Article
Materials Science, Multidisciplinary
Yi Cui, Yuhki Toku, Yasuhiro Kimura, Yang Ju
Summary: Through large-scale atomistic simulations, it is proven that unclosed shear dislocation loops play a crucial role in cold-welded metallic nanowires, affecting not only their tensile deformation but also the fracture process. The emission of shear loops can induce surface deformation and redistribute mass in the process of plasticity, serving as a general mechanism.
COMPUTATIONAL MATERIALS SCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Yuhki Toku, Yosuke Togawa, Yasuyuki Morita, Yang Ju
Summary: This paper introduces a new technique for crystal plane control of single crystal SnO2 nanobelts by thermal sublimation, where synthesis pressure was found to significantly impact the cross-sectional aspect ratio of the nanobelts. Pressure influenced the surface free energy, leading to preferential growth of crystal planes. The aspect ratio range of nanobelts from 1.2 to 7.39 was achieved solely due to pressure variation, with dispersed Au nanoparticles acting as catalysts for the formation of uniform SnO2 nanobelts.
Article
Engineering, Multidisciplinary
Yasumoto Sato, Natsuki Ogura, Yuhei Yamaguchi, Yang Ju
Summary: This study developed a sensor for dielectric constant measurements using a signal transmission line, and validated its effectiveness in evaluating the dielectric constant of resin materials in a simple and convenient manner. The sensor utilized signal reflections caused by variations in the characteristic impedance of the signal transmission line, showing a substantially linear relation between the measured reflection coefficient and the dielectric constant of the resin material measured.
Article
Biochemistry & Molecular Biology
Jie Wang, Lin Liu, Zhongjie Ding, Qing Luo, Yang Ju, Guanbin Song
Summary: Exogenous NAD(+) delays BMSC senescence induced by D-gal by increasing Sirt1 expression, reducing senescence-associated-beta-galactosidase activity, and downregulating poly (ADP-ribose) polymerase 1 expression.
Article
Physics, Applied
Bo Tong, Takahiro Hirabayashi, Yuhki Toku, Yasuyuki Morita, Yang Ju
Summary: A new method was developed in this study to quantitatively evaluate the conductivity of metallic nanowires at nanometer scale using microwave atomic force microscopy. By establishing a semi-near-field model and utilizing a metal strip substrate for calibration, the local conductivities of nanowires were measured in a single scan.
APPLIED PHYSICS EXPRESS
(2021)
Article
Physics, Applied
Minji Zhao, Bo Tong, Yasuhiro Kimura, Yuhki Toku, Yasuyuki Morita, Yang Ju
Summary: This paper presents a non-contact and quantitative method for evaluating the permittivity of semiconductor nanomaterials using microwave atomic force microscopy (M-AFM). The topography and microwave images of nanomaterials were obtained in a single scanning process, allowing for high spatial resolution of ZnO, CuO, and SnO2 nanowires and nanobelts. The local relative permittivity of these one-dimensional metal oxide nanomaterials was quantitatively evaluated.
APPLIED PHYSICS LETTERS
(2021)
Article
Materials Science, Multidisciplinary
Shaojie Gu, Yi Cui, Yasuhiro Kimura, Yuhki Toku, Yang Ju
Summary: The study demonstrates that high-density pulsed electric current treatment can fully relieve strain hardening and restore ductility accordingly; dislocation density plays a dominant role in this process, while grain size has a side effect on strain hardening relief. HDPEC treatment can alter the microstructure of materials, allowing for the enhancement of mechanical properties in deformed components, achieving rapid relief of strain hardening and improved formability.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Sungmin Yoon, Yasuhiro Kimura, Yi Cui, Yuhki Toku, Yang Ju
Summary: This study investigates the evaluation of improved fracture characteristics in stainless steel by HDPEC using finite element analysis. The results suggest that modified material properties by HDPEC positively resist crack propagation.
MATERIALS TRANSACTIONS
(2021)
Review
Chemistry, Multidisciplinary
S. Arumugam, Y. Ju
Summary: The development of suitable bioactive scaffolds for bone tissue engineering is crucial to mimic the extracellular bone matrix, while the use of CNTs-reinforced composites in the biomedical field is increasing due to their outstanding properties.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Mechanics
Yi Cui, Zengtao Chen, Shaojie Gu, Wenzhi Yang, Yang Ju
Summary: The size dependence of central nanovoid embedded in high-entropy-alloy films under biaxial tension is investigated. It is found that lattice distortion facilitates dislocation emission from the void surface, leading to a decrease in critical stress. Additionally, the grain size variation affects the peak stress in polycrystalline samples, and the failure mechanism differs between monocrystalline and polycrystalline samples.
ARCHIVE OF APPLIED MECHANICS
(2023)
Article
Engineering, Electrical & Electronic
Yasuhiro Kimura, Yang Ju
Summary: The study investigated the relationship between intermetallic compounds (IMCs) and electromigration (EM)-based metallic micro/nanowire growth, finding that IMC negatively impacts wire growth. It was discovered that a thicker passivation layer allows for higher pressure generation for wire growth, but overly thick passivation can lead to delamination and hinder wire growth by releasing the interconnect constraints. By sandwiching the metallic interconnect with materials that do not form IMC, it is possible to promote stable EM-based wire growth.
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B
(2021)
Article
Materials Science, Multidisciplinary
Shaojie Gu, Yi Cui, Sungmin Yoon, Zizheng Wang, Yasuhiro Kimura, Yuhki Toku, Yang Ju
Summary: The {101} texture of deformed face-centered cubic metals can be alleviated by high-density pulsed electric current (HDPEC) treatment, which also leads to recovery of deformed grains, resulting in random grain orientation and equiaxed grain morphology to recover anisotropy in the deformed metals. This method provides a promising and cost-effective way to modify the microstructure of materials.
Article
Computer Science, Artificial Intelligence
Zhangyong Li, Wang Chen, Yang Ju, Yong Chen, Zhengjun Hou, Xinwei Li, Yuhao Jiang
Summary: Bone age assessment is crucial for diagnosing endocrinology disorders in adolescents. Many deep learning methods have been proposed for automatic bone age estimation, but they lack sufficient discriminative information or require additional manual annotations. This research proposes a new two-stage deep learning method that extracts critical bone regions and utilizes gender assistance for accurate bone age estimation. The proposed method achieves promising results on both public and private datasets.
FRONTIERS IN ARTIFICIAL INTELLIGENCE
(2023)
Article
Engineering, Mechanical
Sungmin Yoon, Yasuhiro Kimura, Yuhki Toku, Yang Ju, Soojeong Park, Yunhae Kim
Summary: The study evaluated the creep properties of martensitic stainless steel using the LM model, determining creep constants through experiments and analysis. The creep strain-time curves were successfully estimated, characterizing the creep behavior of the material.
MECHANICAL ENGINEERING JOURNAL
(2021)
