Article
Multidisciplinary Sciences
Ze-Hua Xu, Hang Zhang, Chang-Jun Zhang, Si-Jian Yu, Jing Yuan, Kangxin Jin, Zi-Bing Jin
Summary: With the increasing use of artificial light and optoelectronic products, light damage (LD) to the human retina has become a global issue. This study investigated the mechanism of how blue light leads to photoreceptor death and found that regenerating islet-derived 1 alpha (REG1A) prevents LD-induced apoptosis in photoreceptor cells by increasing the expression of the anti-apoptotic gene Bcl2 and decreasing the expression of the pro-apoptotic gene Bax. REG1A restores mitochondrial function and cell apoptosis after LD-induced damage, suggesting its potential application in the prevention and treatment of retinal vision loss.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Cell Biology
Natalia Ziolkowska, Bogdan Lewczuk, Natalia Szyrynska, Aleksandra Rawicka, Alla Vyniarska
Summary: This study examined the impact of low-intensity blue light on the albino Wistar rat retina, specifically the intrinsically photosensitive retinal ganglion cells (ipRGCs). Different exposure patterns of blue light were tested, and the results showed that prolonged exposure to low-intensity blue light caused damage to the retinas of the rats. This damage included decreased dendrite length, reduced immunoreactivity, changes in dendritic arborization, increased GFAP immunoreactivity, and apoptosis.
Article
Ophthalmology
Satoshi Yokota, Sahil H. Shah, Emma Lee Huie, Runxia Rain Wen, Ziming Luo, Jeffrey L. Goldberg
Summary: The study aimed to investigate the dynamics and regulation of mitochondrial transport during retinal ganglion cell (RGC) maturation using acutely purified RGCs as a model system. Single-cell RNA sequencing analysis identified Kinesin family member 5A (Kif5a) as a motor protein relevant to mitochondrial transport. Manipulation of Kif5a expression demonstrated its direct regulation of mitochondrial axonal transport in developing RGCs.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Elisabetta Catalani, Kashi Brunetti, Simona Del Quondam, Davide Cervia
Summary: This article discusses the relationship between mitochondrial dysfunction and retinal pathology related to RGC degeneration, as well as the potential for neuroprotection using redox-related strategies.
Article
Endocrinology & Metabolism
Chao Wang, Yaqiong An, Zhaohua Xia, Xuezhi Zhou, Haibo Li, Shuang Song, Lexi Ding, Xiaobo Xia
Summary: This study found that melatonin could protect RGCs from glutamate excitotoxicity and NMDA-induced injury, reduce oxidative stress, and improve visual function in mice. These findings provide practical research ideas for the comprehensive prevention and treatment of glaucoma using melatonin.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Ben Mead, Alicia Kerr, Naoki Nakaya, Stanislav I. Tomarev
Summary: This study aimed to characterize the miRNA profile of purified retinal ganglion cells (RGC) from healthy and diseased rat retina. Over 100 different miRNAs were identified in diseased retina compared to healthy retina. Manipulating candidate mRNAs after retinal injury revealed significant neuroprotection and neuritogenesis effects in RGC.
Review
Biochemistry & Molecular Biology
Eugene Yu-Chuan Kang, Pei-Kang Liu, Yao-Tseng Wen, Peter M. J. Quinn, Sarah R. Levi, Nan-Kai Wang, Rong-Kung Tsai
Summary: RGC degeneration is the most common neurodegenerative disorder causing irreversible blindness worldwide, with the underlying pathophysiology and mechanisms largely unknown. Oxidative stress and mitochondrial dysfunction have been identified as principal mechanisms in RGC degeneration and other neurodegenerative disorders. Antioxidants may hold promise as a potential future therapy for ocular diseases associated with RGC degeneration.
Article
Engineering, Biomedical
Sanaz Behtaj, Fereshteh Karamali, Samaneh Najafian, Elahe Masaeli, Mohammad-Hossein Nasr Esfahani, Maksym Rybachuk
Summary: Stem cell-based retinal ganglion cells (RGCs) replacement therapy shows potential for restoring vision in glaucoma by replacing degenerated RGCs and facilitating axonal regeneration. However, generating clinically safe and functional RGCs that can integrate into hosts' retinas remains a challenge. The use of PGS/PCL scaffold in differentiation of hESCs into RGC-like cells promotes cell differentiation and neurite growth, contributing to potential future clinical therapy for glaucoma treatment.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Elena Grossini, Sakthipriyan Venkatesan, Micol Alkabes, Caterina Toma, Stefano de Cilla
Summary: Research has shown that Membrane Blue Dual (MBD) exerts greater protective effects, especially when combined with polyethylene glycol (PEG), by modulating mitochondrial function to increase cell viability, mitochondrial membrane potential, proliferation, and migration in human retinal pigment epithelial cells (ARPE-19) and retinal ganglion cells-like (RGC-5).
Article
Ophthalmology
Ru Zhao, Tao He, Yiqiao Xing, Jinyuan Luo
Summary: This study finds that COG1410 regulates the state of microglia and protects retinal ganglion cells in retinal ischemia-reperfusion injury, showing promising potential for the treatment of eye diseases.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Cell Biology
Jennifer H. Pham, Gretchen A. Johnson, Rajiv S. Rangan, Charles E. Amankwa, Suchismita Acharya, Dorota L. Stankowska
Summary: This study evaluated the mechanisms underlying the neuroprotective effects of the compound SA-2 in retinal ganglion cell degeneration models. The results showed that SA-2 reduced apoptosis and decreased reactive oxygen species (ROS) levels in rat and human RGCs, suggesting that it may be a potential therapeutic strategy for preventing neurodegeneration in glaucomatous RGCs.
Article
Ophthalmology
You-Ren Wu, Tomoyo Hashiguchi, Junki Sho, Shih-Hwa Chiou, Masayo Takahashi, Michiko Mandai
Summary: The study shows the potential for therapeutic use of pluripotent cell-derived RGCs by grafting the cells in healthy conditions and with an appropriate technical approach. Transplanted mESC-derived RGCs survived and extended neurites into the host retina, forming presumptive synapses with host bipolar cells. The ratio of integrated THY1-EGFP(+) neurites in the host IPL was higher in RGC-injured mice compared to healthy controls.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Cell Biology
Todd McLaughlin, Jinli Wang, Liyun Jia, Fuguo Wu, Yaqin Wang, Joshua J. Wang, Xiuqian Mu, Sarah X. Zhang
Summary: The chaperone p58(IPK) plays a protective role in the retina by reducing retinal ganglion cell (RGC) injury, which is mediated through its expression in retinal neurons. This finding provides a new direction and strategy for the treatment of neurodegenerative retinal diseases.
Article
Ophthalmology
Xiaohui Wang, Zhaowei Song, Huazhang Li, Kexin Liu, Ying Sun, Xiangyu Liu, Meiyu Wang, Yuexing Yang, Sheng Su, Zhijian Li
Summary: The study found that short-wavelength blue light can induce pyroptotic programmed cell death in human lens epithelial cells, and that a caspase-1 inhibitor can suppress this cell death. The dose and time of blue light exposure have a dose-dependent effect on cell death.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Neurosciences
Andrew M. Boal, Nolan R. McGrady, Joseph M. Holden, Michael L. Risner, David J. Calkins
Summary: By studying an inducible mouse model of glaucoma, it was found that the sensitivity to increased K+ concentration is reduced and the excitability of RGCs is diminished. These experimental results provide insights into the response of RGCs to intraocular pressure stress and lay the foundation for mechanistic investigation into targets for neuroprotective therapy.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Ophthalmology
Claudia Nunez-Alvarez, Carlota Suarez-Barrio, Susana del Olmo Aguado, Neville N. Osborne
ACTA OPHTHALMOLOGICA
(2019)
Article
Ophthalmology
Claudia Nunez-Alvarez, Neville N. Osborne
EXPERIMENTAL EYE RESEARCH
(2019)
Letter
Clinical Neurology
Luis Santos, Susana del Olmo-Aguado, Pedro L. Valenzuela, Kristian Winge, Eliseo Iglesias-Soler, Juan Arguelles-Luis, Samuel Alvarez-Valle, Guillermo J. Parcero-Iglesias, Ana Fernandez-Martinez, Alejandro Lucia
Article
Biochemistry & Molecular Biology
C. Nunez-Alvarez, N. N. Osborne
NEUROCHEMISTRY INTERNATIONAL
(2019)
Article
Biochemistry & Molecular Biology
Carlota Suarez-Barrio, Susana del Olmo-Aguado, Eva Garcia-Perez, Maria de la Fuente, Francisco Muruzabal, Eduardo Anitua, Begona Baamonde-Arbaiza, Luis Fernandez-Vega-Cueto, Luis Fernandez-Vega, Jesus Merayo-Lloves
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Ophthalmology
Eduardo Anitua, Maria de la Fuente, Susana del Olmo-Aguado, Carlota Suarez-Barrio, Jesus Merayo-Lloves, Francisco Muruzabal
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Carlota Suarez-Barrio, Susana del Olmo-Aguado, Eva Garcia-Perez, Enol Artime, Maria de la Fuente, Francisco Muruzabal, Eduardo Anitua, Begona Baamonde-Arbaiza, Luis Fernandez-Vega, Jesus Merayo-Lloves
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Carlota Suarez-Barrio, Susana del Olmo-Aguado, Eva Garcia-Perez, Luis Fernandez-Vega-Cueto, Andres Fernandez-Vega Cueto, Begona Baamonde-Arbaiza, Luis Fernandez-Vega, Jesus Merayo-Lloves
Summary: Age-related macular degeneration (AMD) can lead to the degeneration of retinal cells and vision loss, and long-term exposure to blue light may exacerbate the progression of AMD. Plasma rich in growth factors (PRGF) has been shown to enhance cell antioxidant pathways, protect photoreceptors from blue light damage, and promote cell autophagy.
Article
Ophthalmology
Andres Fernandez-Vega Cueto, Susana del Olmo-Aguado, Eva Garcia-Perez, Ignacio Rodriguez-Una, Luis Fernandez-Vega Cueto, Carlota Suarez-Barrio, Jose F. Alfonso, Luis Fernandez-Vega, Jesus Merayo-Lloves
Summary: This in vitro study suggests that UV/blue-filtering IOLs are not effective in providing photoprotection under artificial light conditions. Attention should be given to other parameters besides the type of filter when selecting an IOL.
OPHTHALMIC RESEARCH
(2022)
Article
Polymer Science
Ignacio Alcalde, Cristina Sanchez-Fernandez, Susana Del Olmo-Aguado, Carla Martin, Celine Olmiere, Enol Artime, Luis M. Quiros, Jesus Merayo-Lloves
Summary: The study demonstrates the effectiveness of HSmP in promoting corneal epithelial and nerve regeneration, restoring transparency, and reducing myofibroblast scarring after laser experimental injury.
Article
Biochemistry & Molecular Biology
Eduardo Anitua, Francisco Muruzabal, Maria de la Fuente, Susana Del Olmo-Aguado, Mohammad H. H. Alkhraisat, Jesus Merayo-Lloves
Summary: This study evaluates the effectiveness of a novel plasma rich in growth factors (PRGF) membrane in reducing oxidative stress in retinal pigment epithelial cells (ARPE-19 cells) exposed to blue light. Different formulations derived from PRGF were tested on ARPE-19 cells, and it was found that they increased mitochondrial viability, cell survival, and decreased reactive oxygen species (ROS) production and heme oxygenase -1 (HO-1) expression. The PRGF membrane also increased ZO-1 expression in ARPE-19 cells exposed to blue light.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Ignacio Alcalde, Cristina Sanchez-Fernandez, Carla Martin, Nagore De Pablo, Nahla Jemni-Damer, Gustavo V. Guinea, Jesus Merayo-Lloves, Susana Del Olmo-Aguado
Summary: The objective of this study is to analyze the current data from clinical trials using stem cells to treat human retinal diseases. Although there are currently no approved therapies based on stem cell transplantation for patients, the outcomes of many clinical trials show promising results in terms of implantation techniques and long-term safety. Quantifiable results related to visual function improvement are expected in the coming years.
MEDICINA-LITHUANIA
(2022)
Meeting Abstract
Ophthalmology
Alvaro Meana, Natalia Vazquez, Sergio Alonso-Alonso, Carlota Suarez-Barrio, Susana del Olmo Aguado, Manuel Chacon, Eva Garcia-Perez, Mairobi Persinal-Medina, Miguel Naveiras, Jose F. Alfonso, Jesus Merayo-Lloves
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Carlota Suarez Barrio, Susana Del Olmo-Aguado, Claudia Nunez, Maria de la Fuente, Francisco Muruzabal, Gorka Orive, Eduardo Anitua, Neville N. Osborne, Jesus M. Merayo-Lloves
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Meeting Abstract
Ophthalmology
Sabino Padilla, Maria de la Fuente, Francisco Muruzabal, Borja de la Sen, Susana Del Olmo-Aguado, Carlota Suarez-Barrio, Jesus Merayo-Lloves, Eduardo Anitua
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)