Article
Ophthalmology
Emmi Kapiainen, Harri Elamaa, Ilkka Miinalainen, Valerio Izzi, Lauri Eklund
Summary: This study demonstrates the additive effect of Angpt4 in maintaining SC and activating Tie2, as well as the synergistic interaction between Angpt4 and Angpt2. The interplay between the angiopoietins is crucial for maintaining the integrity of the iridocorneal angle.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Medicine, Research & Experimental
Yanan Wang, Wenyan Wang, Xuejiao Yang, Wenshi Chen, Xian Yang, Xiaojing Pan, Peilong Xu, Wei Zhu, Yantao Han, Xuehong Chen
Summary: Cells expressing ITGA8 in the Schlemm's canal region exhibit more properties as Schlemm's canal endothelial cells, indicating a crucial role of ITGA8 in regulating aqueous humor outflow resistance.
Article
Cell Biology
Sze-Wan Shan, Chi-Wai Do, Thomas Chuen Lam, Hoi-Lam Li, W. Daniel Stamer, Chi-Ho To
Summary: The selective ROCK inhibitor Y39983 induces actin remodeling, decreases cell motility and transepithelial resistance in human TM cells. Proteomic analysis identified 20 significantly altered proteins after Y39983 treatment, involving pathways such as p53, integrin signaling, and cytoskeletal regulation by Rho GTPase. Downregulation of TSP1 by Y39983 and its antagonists led to reduced cell migration and increased outflow facility.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qing Sang, Rong Du, Chen Xin, Ningli Wang
Summary: This study preliminarily estimated the effects of pilocarpine eye drops and trabeculotomy tunneling trabeculoplasty (3T) on trabecular meshwork (TM) pulsatile motion. Pilocarpine eye drops attenuated TM pulsatile motion, and 3T surgery reduced intraocular pressure (IOP) without affecting the pulsatile motion status of the TM.
Review
Ophthalmology
Katarzyna Lewczuk, Joanna Jablonska, Joanna Konopinska, Zofia Mariak, Marek Rekas
Summary: This review discusses the physiological mechanisms leading to elevated intraocular pressure in glaucoma due to obstruction of aqueous humor outflow, detailing the pathway of aqueous humor through ocular channels and the crucial role of Schlemm's canal in the pathogenesis of glaucoma. Additionally, an accurate method for assessing the aqueous drainage pathway is introduced.
ACTA OPHTHALMOLOGICA
(2022)
Article
Ophthalmology
Yunsheng Qiao, Zhongmou Sun, Chen Tan, Junyi Lai, Xinghuai Sun, Junyi Chen
Summary: The study demonstrates that AAV-mediated gene therapy can effectively lower intraocular pressure by remodeling the trabecular outflow pathway in mice eyes.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Materials Science, Biomaterials
Maria Bikuna-Izagirre, Javier Aldazabal, Leire Extramiana, Javier Moreno-Montanes, Elena Carnero, Jacobo Paredes
Summary: The researchers developed an in vitro JCT model using a polycaprolactone nanofibrous scaffold, which could recapitulate the functional JCT morphology and study the flow of aqueous humor. The model exhibited HTM-like characteristics and allowed for investigation of the relationship between aqueous humor outflow and HTM degeneration as well as drug screening.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Ophthalmology
Toshit Varshney, Kishan Azmira, Shikha Gupta, Karthikeyan Mahalingam, Abhishek Singh, Dewang Angmo, Viney Gupta
Summary: The presence of Schlemm's canal on ASOCT is a strong predictor for successful intraocular pressure reduction after selective laser trabeculoplasty in patients with juvenile-onset open-angle glaucoma.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Cell Biology
Michael Madekurozwa, W. Daniel Stamer, Ester Reina-Torres, Joseph M. Sherwood, Darryl R. Overby
Summary: Intraocular pressure (IOP) fluctuates due to ocular pulse, which induces shear stress in Schlemm's canal (SC). The ocular pulse may modulate outflow facility by stimulating NO production. Studies showed the immediate increase in outflow facility in mice in response to the ocular pulse, with NO playing a significant role in this regulation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biology
Chen Xin, Shaozhen Song, Ningli Wang, Ruikang Wang, Murray Johnstone
Summary: This study utilized high-resolution OCT to evaluate the impact of Schlemm's canal dilation on the biomechanical properties of the trabecular meshwork and the structure of collector channels. Results demonstrated pressure-dependent changes in the dimensions of Schlemm's canal and collector channels, with elastance curves shifting with increased pressure-dependent steady-state Schlemm's canal area. The study provides insights into the mechanisms of intraocular pressure control in glaucoma surgery.
Article
Biochemistry & Molecular Biology
Magdalena Schneider, Ramona Pawlak, Gregor R. Weber, Andrea E. Dillinger, Sabrina Kuespert, Renato V. Iozzo, Harry A. Quigley, Andreas Ohlmann, Ernst R. Tamm, Rudolf Fuchshofer
Summary: Primary open-angle glaucoma is a neurodegenerative disorder characterized by degeneration of optic nerve axons, with intraocular pressure identified as the major risk factor for its development involving molecular mechanisms like TGF-beta. Decorin has been found to play an important role in intraocular pressure and optic nerve damage.
Article
Ophthalmology
Shayna Sosnowik, David L. L. Swain, Shan Fan, Carol B. B. Toris, Haiyan Gong
Summary: This study examined the morphological changes along the conventional aqueous outflow pathway and their association with decreased outflow facility in an experimental model of glaucoma. The results showed that laser-induced ocular hypertension led to significant changes in the trabecular meshwork and Schlemm's canal, as well as a decrease in the number of intrascleral veins. These findings might contribute to the understanding of glaucoma pathogenesis.
EXPERIMENTAL EYE RESEARCH
(2022)
Review
Cell Biology
Jennifer A. Faralli, Mark S. Filla, Donna M. Peters
Summary: Integrins play an important role in the Trabecular Meshwork/Schlemm's canal of the eye, regulating intraocular pressure and potentially contributing to glaucoma. Intercellular communication between integrins can influence cell response to ECM changes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Elizabeth A. Martin, Murray A. Johnstone
Summary: The study aimed to describe a novel combination of techniques for immunohistochemistry imaging of Schlemm's canal inlet (SIV) and outlet (SOV) valve-like structures, identify the adhesive relationships between trabecular meshwork (TM) and hinged collagen leaflets at the Schlemm's canal (SC) external wall, and determine the similarity between the adhesive vascular markers of the SIV lumen wall and the SC inner wall endothelium. The results showed the 3D relationships between SIV, collector channel ostia, collector channels (CC), SOV, and intrascleral channels, as well as the presence of similar cell-cell adhesion molecules and basement membrane components in the SIV lumen walls and SC inner wall endothelium.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Ophthalmology
Clemens A. Strohmaier, Fiona S. McDonnell, Xiaowei Zhang, Daniel Wanderer, W. Daniel Stamer, Robert N. Weinreb, Alex S. Huang
Summary: This study investigates the differences in outflow facility between high-flow and low-flow segments of the conventional outflow pathway in porcine eyes. It finds that there is an uneven distribution of local outflow facility across different parts of the trabecular meshwork.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Ophthalmology
Kate E. Keller, Sanjoy K. Bhattacharya, Theresa Borras, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark, W. Michael Dismuke, Yiqin Du, Michael H. Elliott, C. Ross Ethier, Jennifer A. Faralli, Thomas F. Freddo, Rudolf Fuchshofer, Michael Giovingo, Haiyan Gong, Pedro Gonzalez, Alex Huang, Murray A. Johnstone, Paul L. Kaufman, Mary J. Kelley, Paul A. Knepper, Casey C. Kopczynski, John G. Kuchtey, Rachel W. Kuchtey, Markus H. Kuehn, Raquel L. Lieberman, Shan C. Lin, Paloma Liton, Yutao Liu, Elke Luetjen-Drecoll, Weiming Mao, Marisse Masis-Solano, Fiona McDonnell, Colleen M. McDowell, Darryl R. Overby, Padmanabhan P. Pattabiraman, Vijay K. Raghunathan, P. Vasanth Rao, Douglas J. Rhee, Uttio Roy Chowdhury, Paul Russell, John R. Samples, Donald Schwartz, Evan B. Stubbs, Ernst R. Tamm, James C. Tan, Carol B. Toris, Karen Y. Torrejon, Janice A. Vranka, Mary K. Wirtz, Thomas Yorio, Jie Zhang, Gulab S. Zode, Michael P. Fautsch, Donna M. Peters, Ted S. Acott, W. Daniel Stamer
EXPERIMENTAL EYE RESEARCH
(2018)
Review
Ophthalmology
M. Elizabeth Fini, Shinwu Jeong, Haiyan Gong, Rafael Martinez-Carrasco, Nora M. Laver, Minako Hijikata, Naoto Keicho, Pablo Argueso
PROGRESS IN RETINAL AND EYE RESEARCH
(2020)
Article
Multidisciplinary Sciences
Amir Vahabikashi, Ariel Gelman, Biqin Dong, Lihua Gong, Elliott D. K. Cha, Margit Schimmel, Ernst R. Tamm, Kristin Perkumas, W. Daniel Stamer, Cheng Sun, Hao F. Zhang, Haiyan Gong, Mark Johnson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Medicine, General & Internal
April Nettesheim, Myoung Sup Shim, Angela Dixon, Urmimala Raychaudhuri, Haiyan Gong, Paloma B. Liton
Summary: The deposition of extracellular matrix (ECM) in the trabecular meshwork (TM) is a hallmark of glaucoma. Previous research has identified the enzyme cathepsin B (CTSB) as playing a role in regulating ECM remodeling and outflow physiology in TM cells, suggesting it as a potential therapeutic target for attenuating fibrosis and ECM deposition in the glaucomatous outflow pathway.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Ophthalmology
David L. Swain, Thuy Duong Le, Senila Yasmin, Beatriz Fernandes, Ganimete Lamaj, Indira Dasgupta, Yanyun Gao, Haiyan Gong
Summary: This study utilized serial block-face scanning electron microscopy to investigate morphological factors associated with GVs with I-pores, revealing a higher presence of GVs with both basal openings and I-pores in high-flow areas, suggesting a potential strategy for glaucoma treatment.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Michael P. Vincent, Trevor Stack, Amir Vahabikashi, Guorong Li, Kristin M. Perkumas, Ruiyi Ren, Haiyan Gong, W. Daniel Stamer, Mark Johnson, Evan A. Scott
Summary: The study presents a targeted drug delivery strategy for treating glaucoma by optimizing FLT4 receptor-targeting ligands, leading to improved treatment outcomes by enhancing uptake by SC cells and reducing uptake by off-target vascular endothelial cells. This approach significantly reduces intraocular pressure and sustains the effect for a longer period of time compared to conventional treatments. Confocal microscopy confirms the localization of nanocarriers to SC within 1 hour of administration, showing the potential for targeted drug delivery in glaucoma therapy.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Ruiyi Ren, Anne A. Humphrey, David L. Swain, Haiyan Gong
Summary: This study investigated the relationship between intraocular pressure (IOP) and effective filtration area (EFA) in a mouse model of steroid-induced ocular hypertension. The researchers found that a reduction in EFA correlated with an increase in IOP, suggesting that morphological changes in the trabecular meshwork contribute to elevated IOP in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Ophthalmology
Shayna Sosnowik, David L. L. Swain, Shan Fan, Carol B. B. Toris, Haiyan Gong
Summary: This study examined the morphological changes along the conventional aqueous outflow pathway and their association with decreased outflow facility in an experimental model of glaucoma. The results showed that laser-induced ocular hypertension led to significant changes in the trabecular meshwork and Schlemm's canal, as well as a decrease in the number of intrascleral veins. These findings might contribute to the understanding of glaucoma pathogenesis.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Cell Biology
David L. Swain, Senila Yasmin, Beatriz Fernandes, Ganimete Lamaj, Yanfeng Su, Haiyan Gong
Summary: Research has found that a decreased number of cellular connections between the inner wall (IW) endothelial cells and the juxtacanalicular connective tissue (JCT) in high-flow areas of the eye is associated with larger giant vacuoles and fewer connections. This suggests that modulating the number of cellular connections can affect the amount of high-flow area around the eye and thereby modulate aqueous humor outflow.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Ophthalmology
Meenakashi Gupta, Jae Young Heo, Haiyan Gong, Elliot Cha, Mark Latina, Douglas J. Rhee
Summary: This study evaluated the morphologic and cellular changes as well as DNA synthesis after SLT treatment of human trabecular meshwork tissue. The results showed that SLT treatment caused ultrastructural changes, increased mitotic activity, and DNA synthesis in the trabecular meshwork.
CLINICAL OPHTHALMOLOGY
(2022)
Article
Cell Biology
Shayna Sosnowik, David L. Swain, Neil Liu, Shan Fan, Carol B. Toris, Haiyan Gong
Summary: This study investigated the glycocalyx morphology in the trabecular outflow pathway of monkey eyes with experimental glaucoma. The results showed decreased outflow facility in laser-treated eyes and significant changes in the glycocalyx coverage and thickness in different regions.
Article
Ophthalmology
Ruiyi Ren, Anne A. Humphrey, Casey Kopczynski, Haiyan Gong
Summary: In this study, researchers investigated the mechanisms of reducing intraocular pressure (IOP) in steroid-induced ocular hypertension (SIOH) using a Rho kinase inhibitor called AR-12286. The results showed that AR-12286 effectively reduced IOP in SIOH eyes, and it was more effective than discontinuing steroid treatment.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Ophthalmology
Colleen M. McDowell, Krishnakumar Kizhatil, Michael H. Elliott, Darryl R. Overby, Joseph Van Batenburg-Sherwood, J. Cameron Millar, Markus H. Kuehn, Gulab Zode, Ted S. Acott, Michael G. Anderson, Sanjoy K. Bhattacharya, Jacques A. Bertrand, Terete Borras, Diane E. Bovenkamp, Lin Cheng, John Danias, Michael Lucio De Ieso, Yiqin Du, Jennifer A. Faralli, Rudolf Fuchshofer, Preethi S. Ganapathy, Haiyan Gong, Samuel Herberg, Humberto Hernandez, Peter Humphries, Simon W. M. John, Paul L. Kaufman, Kate E. Keller, Mary J. Kelley, Ruth A. Kelly, David Krizaj, Ajay Kumar, Brian C. Leonard, Raquel L. Lieberman, Paloma Liton, Yutao Liu, Katy C. Liu, Navita N. Lopez, Weiming Mao, Timur Mavlyutov, Fiona McDonnell, Gillian J. McLellan, Philip Mzyk, Andrews Nartey, Louis R. Pasquale, Gaurang C. Patel, Padmanabhan P. Pattabiraman, Donna M. Peters, Vijaykrishna Raghunathan, Ponugoti Vasantha Rao, Naga Rayana, Urmimala Raychaudhuri, Ester Reina-Torres, Ruiyi Ren, Douglas Rhee, Uttio Roy Chowdhury, John R. Samples, E. Griffen Samples, Najam Sharif, Joel S. Schuman, Val C. Sheffield, Cooper H. Stevenson, Avinash Soundararajan, Preeti Subramanian, Chenna Kesavulu Sugali, Yang Sun, Carol B. Toris, Karen Y. Torrejon, Amir Vahabikashi, Janice A. Vranka, Ting Wang, Colin E. Willoughby, Chen Xin, Hongmin Yun, Hao F. Zhang, Michael P. Fautsch, Ernst R. Tamm, Abbot F. Clark, C. Ross Ethier, W. Daniel Stamer
Summary: This article highlights the importance of using mice as a valuable model system for studying conventional outflow resistance and intraocular pressure. It also outlines the minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. These standards are expected to enhance scientific rigor and replicability of research findings.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Julia Lai, Yanfeng Su, David L. Swain, Davy Huang, Dimitr Getchevski, Haiyan Gong
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Article
Ophthalmology
Yiwen Wang, Deborah Osakue, Enzhi Yang, Yi Zhou, Haiyan Gong, Xiaobo Xia, Yiqin Du
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)