Journal
EXPERIMENTAL DERMATOLOGY
Volume 24, Issue 1, Pages 78-80Publisher
WILEY-BLACKWELL
DOI: 10.1111/exd.12553
Keywords
burns; collagen; fibre alignment; image analysis; scar remodelling
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Funding
- NIH [F32AR061933, R01EB007542]
- American Cancer Society [RSG-09-174-01-CCE]
- Shriners Grant [85120-BOS]
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [F32AR061933] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB007542] Funding Source: NIH RePORTER
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Standard approaches to evaluate scar formation within histological sections rely on qualitative evaluations and scoring, which limits our understanding of the remodelling process. We have recently developed an image analysis technique for the rapid quantification of fibre alignment at each pixel location. The goal of this study was to evaluate its application for quantitatively mapping scar formation in histological sections of cutaneous burns. To this end, we utilized directional statistics to define maps of fibre density and directional variance from Masson's trichrome-stained sections for quantifying changes in collagen organization during scar remodelling. Significant increases in collagen fibre density are detectable soon after burn injury in a rat model. Decreased fibre directional variance in the scar was also detectable between 3weeks and 6months after injury, indicating increasing fibre alignment. This automated analysis of fibre organization can provide objective surrogate endpoints for evaluating cutaneous wound repair and regeneration.
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