Journal
EXPERIMENTAL DERMATOLOGY
Volume 23, Issue 4, Pages 272-273Publisher
WILEY
DOI: 10.1111/exd.12362
Keywords
multiplex technology; protein-protein interaction; signal transduction; skin-resident T cell; T cell antigen receptor
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Funding
- National Institutes of Health [1R01GM103841-01A1]
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Studying signal transduction in skin-resident T cells (sr-T cells) can be limited by the small size of clinical biopsies. Here, we isolated sr-T cells from clinical samples and analysed signalling protein complexes by multiplex immunoprecipitation detected by flow cytometry (mIP-FCM). In samples from two independent donors, antigenic stimulation induced signalling proteins to join shared complexes that were observed in seven pairwise combinations among five proteins. This demonstrates that sr-T cells isolated from small clinical samples provide sufficient material for mIP-FCM-based analysis of signalling-induced protein complexes. We propose that this strategy may be useful for gaining improved mechanistic insight of sr-T cell signal transduction associated with dermatological disease.
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