Journal
EXPERIMENTAL CELL RESEARCH
Volume 326, Issue 1, Pages 36-45Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2014.06.004
Keywords
Amyotrophic lateral sclerosis; Cabeza; Retina; EGFR pathway; Drosophila
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Funding
- Japan Science and Technology Agency
- Ministry of Education, Science, Sports and Culture of Japan
- JSPS Core-to-Core Program
- Grants-in-Aid for Scientific Research [24406030] Funding Source: KAKEN
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Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease that causes progressive muscular weakness. Fused in Sarcoma (FUS) that has been identified in familial ALS is an RNA binding protein that is normally localized in the nucleus. However, its function in vivo is not fully understood. Drosophila has Cabeza (Caz) as a FUS homologue and specific knockdown of Caz in the eye imaginal disc and pupal retina using a GMR-GAL4 driver was here found to induce an abnormal morphology of the adult compound eyes, a rough eye phenotype. This was partially suppressed by expression of the apoptosis inhibitor P35. Knockdown of Caz exerted no apparent effect on differentiation of photoreceptor cells. However, immunostaining with an antibody to Cut that marks cone cells revealed fusion of these and ommatidia of pupal retinae. These results indicate that Caz knockdown induces apoptosis and also inhibits differentiation of cone cells, resulting in abnormal eye morphology in adults. Mutation in EGFR pathway-related genes, such as rhomboid-1, rhomboid-3 and mirror suppressed the rough eye phenotype induced by Caz knockdown. Moreover, the rhomboid-1 mutation rescued the fusion of cone cells and ommatidia observed in Caz knockdown flies. The results suggest that Caz negatively regulates the EGFR signaling pathway required for determination of cone cell fate in Drosophila. (C) 2014 Elsevier Inc. All rights reserved.
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