Journal
EXPERIMENTAL CELL RESEARCH
Volume 314, Issue 7, Pages 1507-1518Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2008.01.010
Keywords
histone deacetylase inhibitors; tumor necrosis factor alpha; NF-kappa B; apoptosis, histone deacetylase 2
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HDAC inhibitors exert potent anti-tumorigenic and anti-inflammatory activity. Their effects are selective for transformed cells, and we recently demonstrated that transformation of epithelial cells with k-Ras sensitizes cells to HDACi induced apoptosis. The aim of this study was to determine whether the ability of HDACi to modulate signaling by a major proinflammatory cytokine, TNF alpha, is also restricted to cells that harbor mutant k-Ras. We used the system of two isogenic cell lines that differ by the presence of mutant k-Ras, HCT116 and Hke3 cells. Treatment of cells with TNF alpha alone did not induce apoptosis; however HDACi potentiated TNF alpha-induced apoptosis in both HCT116 and Hke3 cells. Thus, the ability of HDACi to sensitize cells to TNF alpha-induced apoptosis appears to be k-Ras independent. We demonstrated that HDACi inhibited TNFa-induced NF-kappa B transcriptional and DNA binding activity in both cell lines, underlying the increased apoptosis in cells treated with both agents. We showed that overexpression of HDAC2 enhanced TNFa-induced NF-kappa B activity and that silencing of HDAC2 decreased NF-kappa B activity. Finally, silencing of HDAC2 expression was sufficient to sensitize colon cancer cells to TNF alpha-induced apoptosis. The ability of HDACi to interfere with NF-kappa B activity is likely to contribute to their potent anti-tumorigenic and anti-inflammatory activity. (C) 2008 Elsevier Inc. All rights reserved.
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