Journal
EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 236, Issue 6, Pages 692-700Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1258/ebm.2011.010300
Keywords
immortalization; telomerase; endothelial cells; hTERT
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Funding
- German Ministry of Education and Research (BMBF) [01GU0727]
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Telomerase reverse transcriptase (TERT) is fundamental in determining the life span by regulating telomere length of chromosomes. To address the question whether the enhancement of the proliferative potential hampers cell differentiation, we generated TEAT-over-expressing endothelial cells (ECs) and analyzed in vitro their (1) barrier function; (2) low-density lipoprotein uptake; (3) expression pattern of six selected marker proteins; (4) angiogenic potential in four assays; and (5) inflammatory responses. In contrast to investigations with focus on other cell parameters, we demonstrate that immortalization of ECs by over-expression of TEAT resulted in different angiogenic and inflammatory behavior in comparison to cells with low native telomerase levels.
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