Journal
EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 235, Issue 12, Pages 1442-1449Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1258/ebm.2010.010167
Keywords
Sibiraea angustata; 373-L1; adipocyte differentiation; adipogenesis; obesity
Categories
Funding
- National Natural Science Foundation of China [30870926]
- Key Laboratory of Beijing Normal University
- Key Laboratory for Cell Proliferation and Regulation Biology, Ministry of Education
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Obesity has become a major health concern due to its strong association with the metabolic syndrome. Inhibition of adipocyte differentiation represents a key strategy to inhibit obesity. Sibiraea angustata (SA), a traditional Chinese herb, has a wide range of pharmacological effects, such as improving digestive functions. Here, we report a novel antiadipogenic effect of SA. By using the SA water extract (SAW), SA acetic ether extract (SAA) and the 3T3-L1 model of adipocyte differentiation and adipogenesis, we showed that both SAW and SAA impaired the proliferation and adipo-differentiation of 3T3-L1 in a dose-and time-dependent manner. At the molecular level, treatment of 313-L1 cells with SAW or SAA inhibited the expression of the key adipocyte differentiation regulator CCAAT enhancer binding protein beta (C/EBP beta), as well as peroxisome proliferator activated receptor gamma, adipocyte protein-2, lipoprotein lipase and glucose transporter 4. Cell cycle analysis showed that both SAW and SAA blocked cell cycle at the G1-S transition phase, causing cells to remain in the preadipocyte state. The expression of CyclinA and cyclin-dependent kinase 2 was also inhibited by SAW and SAA. Treatment with SAW also prevented the localization of C/EBP beta to the centromeres. Taken together, our results show that SA has a potent antiadipogenic effect in 3T3-L1 cells due to the inhibition of adipocyte differentiation and adipogenesis. We propose that SA may be used as a safe and effective neutraceutical to manage obesity.
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