Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Agriculture, Dairy & Animal Science
Yao Xiao, Froylan Sosa, Lesley R. de Armas, Li Pan, Peter J. Hansen
Summary: Gene expression analysis in preimplantation embryos using a direct synthesis and specific-target pre-amplification method showed reliable results for sexing and other gene expression studies. Calibration curve analysis of PCR results validated 93.75% of genes tested, demonstrating the method's robustness. The study also showed that within-assay variation increased when cycle threshold values exceeded 18, indicating limitations in sensitivity at higher levels of gene expression.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Virology
Yuka Horio, Mototada Shichiri, Yuji Isegawa
Summary: A novel method was developed to evaluate mRNA transcription activity of influenza RdRp without the use of ultracentrifugation and radioisotopes. The method showed specificity and sensitivity in quantifying mRNA synthesis, even at low levels, and was able to demonstrate the inhibition of mRNA synthesis by an RdRp inhibitor. This new approach will facilitate further research on identifying compounds that inhibit viral mRNA transcription activity of RdRp.
Article
Biochemical Research Methods
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hyejeong Jeong, Ki-Hun Jeong
Summary: A multi-channel plasmonic real-time reverse-transcription polymerase chain reaction system is used to demonstrate high-throughput and multi-target detection of the SARS-CoV-2 virus with four simultaneous reactions in a single assay within 15 min.
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Rokshana Parvin, Lexiang Zhang, Yan Zu, Fangfu Ye
Summary: Exosomal microRNAs have great potential as noninvasive biomarkers, and a duplex photothermal digital PCR strategy combined with a lipid nanoparticle-based exosome capture approach was proposed in this study. Microfluidically-generated molybdenum disulfide-nanocomposite-doped gelatin microcarriers were used for PCR thermocycling triggered by near-infrared, allowing for efficient profiling of microRNAs expression. The method showed promising results for liver cancer diagnostics.
Article
Infectious Diseases
Jinjuan Zhang, Caifang Bu, Liwei Yao, Kan Xu
Summary: The study assessed the accuracy of the MTB-RNA assay using pericardial tissue specimens for diagnosing tuberculous pericarditis. The results showed that the diagnostic accuracy of the MTB-RNA assay was high for culture-positive tuberculous pericarditis, but low for culture-negative cases.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Chemistry, Analytical
Tchamie Kadja, Yvonne Sun, Vamsy P. Chodavarapu
Summary: Rapid and cost-effective biological sample testing systems are crucial for point-of-care diagnostics and other health applications. The recent COVID-19 pandemic highlighted the urgent need for accurate and rapid identification of the genetic material of SARS-CoV-2 in respiratory specimens. Current extraction methods are expensive and time-consuming, but our proposed heat-mediated enzymatic assay improves PCR reaction sensitivity and offers a viable alternative.
Article
Health Care Sciences & Services
Lucian Giubelan, Ilona Stanciu, Cristina Ilie, Vlad Padureanu
Summary: The aim of this paper is to report a case of a newly diagnosed HIV-infected patient with severe immunosuppression who also contracted COVID-19 and had a long duration of positive RT-PCR results for RNA SARS-CoV-2. Indirect evidence suggests that viable virus persisted for a prolonged period in the absence of sufficient host defenses. Improvements in immune function following antiretroviral treatment did not result in an increased inflammatory response to the coronavirus infection and appeared to be the main factor in controlling the infection. Remdesivir, used to treat SARS-CoV-2 infection, did not have an immediate effect on the recorded cycle-threshold.
Article
Pharmacology & Pharmacy
M. Ulas, E. Akbas, S. Akbas, G. Aktemur, N. Durcanoglu, K. Aksak, A. A. Atalar, S. Yildirim, I. Akalin
Summary: This study investigates the levels and importance of miRNA expression during fasting. It was found that miRNA modulates apoptotic pathways to have anti-pathogenic effects and decrease the adaptation of abnormal cells. By down-regulating miRNA expression, programmed cell death is increased to prevent the proliferation and growth of cancerous cells, suggesting potential therapeutic applications.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)