Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xiaowei Song, Qingce Zang, Chao Li, Tianhao Zhou, Richard N. N. Zare
Summary: We developed an immunoassay-based desorption electrospray ionization mass spectrometry imaging (immuno-DESI-MSI) technique for visualizing functional macromolecules. Boronic acid mass tags (BMTs) were synthesized to label antibodies as MSI probes, which can be released from the antibody by cleaving the boronic ester bond under acidic conditions. The BMTs can work in both optical and MS imaging modes and can be readily identified due to the introduction of the boron element.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Xue Zhao, Jiaqi Liang, Ziye Chen, Ruijun Jian, Yao Qian, Yunfang Wang, Zhiying Guo, Wenpeng Zhang, Ying Zhang, Hang Yin, Yu Xia
Summary: LPCAT1 plays a critical role in the remodeling of PCs in cellular lipidome, and it shows exclusive sn-1 specificity regardless of the identity of acyl-CoAs. Increased expression of LPCAT1 in HCC tissue leads to elevated PC 18:1/16:0 relative to its sn-isomer, and MS imaging enables molecular-level visualization of HCC margins in human liver tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Siwen Liang, Deniz Seven, Nevena Prodanovic, Julia Sundheimer, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: The development and validation of desorption/ionization mass spectrometric assays for drug quantification in tissue sections is crucial for their widespread application in (clinical) pharmacology. Advances in desorption electrospray ionization have demonstrated its usefulness for developing targeted quantification methods that meet validation requirements. Factors such as desorption spot morphology, analytical time, and sample surface need to be considered for successful method development. This study highlights the importance of considering desorption kinetics during DESI analyses, which can help reduce analytical time, verify solvent-based drug extraction methods, and predict feasibility of imaging assays. These findings provide valuable guidance for future development of validated DESI-profiling and imaging methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for creating and monitoring rapid oxidation reactions without the addition of external oxidants or catalysts. The oxidation products from the MPTIR match those of the long-term environmental oxidative process, and two unreported environmental compounds were identified in actual water samples using this device.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Margaux Fresnais, Siwen Liang, Marius Breitkopf, Joshua Raoul Lindner, Emmanuelle Claude, Steven Pringle, Pavel A. Levkin, Konstantin Demir, Julia Benzel, Julia Sundheimer, Britta Statz, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: Desorption/ionization-mass spectrometric methods are advantageous for the analysis of solid biological matrices. The development of drug quantification assays using DESI has been challenging, but new prototype source setups show promise for targeted quantitative analyses.
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Biochemistry & Molecular Biology
Xianlin Han, Richard W. Gross
Summary: This article introduces the foundations, advances, and future directions of the lipidomics field. Technological breakthroughs in the 1980s laid the groundwork for lipidomics and subsequent innovations in fragmentation, resolution, and sample analysis drove its rapid growth. Strategic approaches, such as direct infusion and chromatographic separation, facilitated the study of low abundance lipid species.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Chemistry, Analytical
Liang Chen, Alireza Ghiasvand, Shing Chung Lam, Estrella Sanz Rodriguez, Peter C. Innis, Brett Paull
Summary: A thread-based isotachophoresis method coupled with desorption electrospray ionization mass spectrometry (TB-ITP-DESI-MS) was developed for clean-up, preconcentration, and determination of alkaloids in biological fluids. The TB-ITP platform components were fabricated using 3D printing, with a nylon thread as the electrophoresis substrate and a cotton knot as the trapping zone. The method showed improved signal to noise ratio compared to direct DESI-MS detection, making it a viable solution for the analysis of complex biological fluid samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Gwangbin Lee, Sangwon Cha
Summary: Nano-DESI MS has been explored in the forensic chemical analysis of ink directly from handwriting on paper, showing the potential for changing mass spectral profiles over time to reflect the differential distribution of analytes in a vertical direction. The study confirmed the capability of the depth-dependent analysis of nano-DESI MS.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Benjamin Bartels, Ales Svatos
Summary: The study evaluates the application of laser ablation electrospray ionization mass spectrometry imaging (LAESI-MSI) in the field of chemical ecology, and finds that the repeatability of LAESI-MSI in current implementations is insufficient.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Letter
Oncology
Jevon A. Cutler, Savita Udainiya, Anil K. Madugundu, Santosh Renuse, Yaoyu Xu, Jaehun Jung, Kwang Pyo Kim, Xinyan Wu, Akhilesh Pandey
Article
Chemistry, Analytical
Yeon Choi, Kyowon Jeong, Sanghee Shin, Joon Won Lee, Young-Suk Lee, Sangtae Kim, Sun Ah Kim, Jaehun Jung, Kwang Pyo Kim, V. Narry Kim, Jong-Seo Kim
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Jae Hun Jung, Kohei Taniguchi, Hyeong Min Lee, MinYoung Lee, Raju Bandu, Kazumasa Komura, KilYeon Lee, Yukihiro Akao, Kwang Pyo Kim
SCIENTIFIC REPORTS
(2020)
Article
Biochemical Research Methods
Ji-Hyun Lee, Jae Hun Jung, Jeesoo Kim, Won-Ki Baek, Jinseol Rhee, Tae-Hwan Kim, Sang-Hyon Kim, Kwang Pyo Kim, Chang-Nam Son, Jong-Seo Kim
CLINICAL PROTEOMICS
(2020)
Article
Endocrinology & Metabolism
Eu Jeong Ku, Kyung-Cho Cho, Cheong Lim, Jeong Won Kang, Jae Won Oh, Yu Ri Choi, Jong-Moon Park, Na-Young Han, Jong Jin Oh, Tae Jung Oh, Hak Chul Jang, Hookeun Lee, Kwang Pyo Kim, Sung Hee Choi
BMJ OPEN DIABETES RESEARCH & CARE
(2020)
Article
Biochemistry & Molecular Biology
Sung-Jun Hong, Jin-Tae Kim, Su-Jung Kim, Nam-Chul Cho, Kyeojin Kim, Seungbeom Lee, Young-Ger Suh, Kyung-Cho Cho, Kwang Pyo Kim, Young-Joon Surh
Article
Cell Biology
Eun-Gyung Cho, Suh-Yeon Choi, Hyoseon Kim, Eun-Jeong Choi, Eun-Jeong Lee, Phil-Jun Park, Jaeyoung Ko, Kwang Pyo Kim, Heung Soo Baek
Summary: Ginseng-derived EVs have shown the ability to improve senescent phenotypes and melanogenesis in human skin cells, with a unique lipidomic profile compared to parental root extracts. These findings suggest that GEVs could be utilized as novel bioactive nanomaterials for targeted delivery of active constituents in human tissues.
Article
Cell Biology
Bitnara Han, Byung-Kwon Jung, So-Hyun Park, Kyu Jin Song, Muhammad Ayaz Anwar, Kwon-Yul Ryu, Kwang Pyo Kim
Summary: Analysis of the testis proteome in Ubb knockout mice revealed 564 proteins significantly differentially expressed, with 36 downregulated proteins involved in various stages of spermatogenesis. Functional gene ontology analysis identified decreased levels of piRNA metabolic process-related proteins, such as Piwil2 and Tdrd1, in Ubb-null testes. Protein-protein interaction mapping showed that 24 testis development-related proteins, including Hsp90aa1, Eef1a1, and Pabpc1, were directly impacted by the depletion of ubiquitin, with mRNA levels of these proteins reduced in Ubb-knockout testes resembling the global downregulation of piRNA-metabolic gene expression at transcriptional and post-transcriptional levels.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Jong-Kwang Kim, Jae-Hun Jung, Dong-Hoon Shin, Hye-Jin You, Seho Cha, Bo-Seul Song, Jae-Young Joung, Weon-Seo Park, Kwang-Pyo Kim, Jae-Kyung Myung
Summary: This study characterized the rapid proteomic changes in VCaP cells after androgen treatment, identifying five protein clusters involved in different stages of androgen-initiated signal transmission and processing. The study revealed significant regulation of AR-regulated proteins in CRPC patients and provided a comprehensive perspective on the molecular mechanisms underlying CRPC progression.
Article
Biochemistry & Molecular Biology
Yosuke Inomata, Jae-Won Oh, Kohei Taniguchi, Nobuhiko Sugito, Nao Kawaguchi, Fumitoshi Hirokawa, Sang-Woong Lee, Yukihiro Akao, Shinji Takai, Kwang-Pyo Kim, Kazuhisa Uchiyama
Summary: The pathophysiology of non-alcoholic steatohepatitis (NASH) was investigated from the perspective of glycolysis and the Warburg effect, with a focus on microRNA regulation in liver-specific macrophages. The activation of glycolysis in Kupffer cells during NASH was partially induced by the upregulation of pyruvate kinase muscle 2 (PKM2) via microRNA122-5p (miR-122-5p) suppression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Yang Gyun Kim, Sang Ho Lee, Su Woong Jung, Gun Tae Jung, Hyun Ji Lim, Kwang Pyo Kim, Young-Il Jo, KyuBok Jin, Ju Young Moon
Summary: This study assessed the efficacy of protein-bound uremic toxins removal in different dialysis modalities, including high-flux HD, post-dilution online HDF, and MCO-HD. Results showed that post-OL-HDF had the highest clearance rate for B2MG, while MCO-HD was most effective for lambda-FLC clearance. The removal of IS and pCS did not differ significantly among the three dialysis groups.
Correction
Biochemical Research Methods
Ji-Hyun Lee, Jae Hun Jung, Jeesoo Kim, Won-Ki Baek, Jinseol Rhee, Tae-Hwan Kim, Sang-Hyon Kim, Kwang Pyo Kim, Chang-Nam Son, Jong-Seo Kim
CLINICAL PROTEOMICS
(2023)
Article
Chemistry, Multidisciplinary
Muhammad Ayaz Anwar, Muhammad Haseeb, Sangdun Choi, Kwang Pyo Kim
Summary: Maspin is a protein that regresses tumors by inhibiting angiogenesis, but its effects depend on the context and sequence. Various proteins and cofactors bind to maspin, which may explain its conflicting roles. Polymorphic forms of maspin have also been linked to tumor regression and survival, with different amino acids at position 176 having opposing effects in cancer development. Molecular dynamics simulations have revealed a possible link between polymorphic forms and tumor progression, showing that altered electrostatics affect the localization and preference of maspin-binding partners, leading to different maspin-protein(companion)-interaction landscapes.
Article
Biochemistry & Molecular Biology
Hyoseon Kim, Minjung Kim, Kilsun Myoung, Wanil Kim, Jaeyoung Ko, Kwang Pyo Kim, Eun-Gyung Cho
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Ophthalmology
Se Hyun Choi, Jae Won Oh, Jin Suk Ryu, Hye Min Kim, Sin-Hyeog Im, Kwang Pyo Kim, Mee Kum Kim
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)