Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Hematology
Chrysa Kapeni, Leslie Nitsche, Alastair M. Kilpatrick, Nicola K. Wilson, Kankan Xia, Bahar Mirshekar-Syahkal, Vashe Chandrakanthan, Camille Malouf, John E. Pimanda, Berthold Gottgens, Kristina Kirschner, Simon R. Tomlinson, Katrin Ottersbach
Summary: This study shows that the cell cycle inhibitor p57Kip2/Cdkn1c limits the number of hematopoietic stem cells by restricting the size of the sympathetic nervous system and the amount of catecholamines secreted by these cells. This regulation occurs at the sympathetic nervous system progenitor level and has a significant impact on early fetal liver colonization.
Review
Pharmacology & Pharmacy
Edward J. Calabrese
Summary: This paper provides a detailed assessment of hormetic dose responses by human periodontal ligament stem cells, induced by a broad range of chemicals. The underlying mechanistic foundations of these dose responses are clarified, showing them to be widespread and highly conserved across different types of stem cells. The therapeutic implications of these hormetic dose responses are explored, highlighting their potential in various applications.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Biology
Afshin Zare, Aria Salehpour, Arezoo Khoradmehr, Shabnam Bakhshalizadeh, Vahid Najafzadeh, Sahar Almasi-Turk, Mahdi Mahdipour, Reza Shirazi, Amin Tamadon
Summary: More research is being conducted on using stem cell lines to develop into cardiomyocytes for myocardial cell treatments. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have shown promise in treating various cardiac illnesses. This study explores the differentiation of these cells into cardiomyocytes from an epigenetic perspective, including histone acetylation, methylation, DNA alterations, RNA methylation, and mitochondrial mutations.
Article
Cell & Tissue Engineering
Beatrice Xuan Ho, Hongbing Yu, Jeremy Kah Sheng Pang, Jin-Hui Hor, Lee Chuen Liew, Piotr Szyniarowski, Christina Ying Yan Lim, Omer An, Henry He Yang, Colin L. Stewart, Woon Khiong Chan, Shi-Yan Ng, Boon-Seng Soh
Summary: The study found that treatment with IFN-g can promote the maturation of human pluripotent stem cell-derived cardiomyocytes, enhancing their electrophysiological characteristics and sarcomeric structure, and this effect can be nullified with a JAK-STAT pathway inhibitor.
Article
Multidisciplinary Sciences
Ryuki Shimada, Hiroko Koike, Takamasa Hirano, Yuzuru Kato, Yumiko Saga
Summary: During murine germ cell development, NANOS2 plays a key role in suppressing the cell cycle and initiating sexually dimorphic differentiation. Using single-cell RNA sequencing, it was revealed that NANOS2 inhibits mTORC1 activity to suppress the cell cycle, potentially through target proteins like Ptma.
Article
Cell & Tissue Engineering
Yikai Huang, Ting Su, Congcong Wang, Lixia Dong, Shuang Liu, Yaru Zhu, Kunying Hao, Yin Xia, Qing Jiang, Jinzhong Qin
Summary: The deficiency of retinoblastoma binding protein 4 (RBBP4) in embryonic stem cells (ESCs) leads to spontaneous differentiation into mesendodermal lineages, and shares common genomic targets with the core Polycomb complex. RBBP4 is crucial for targeting PRC2 to a subset of developmental genes, and its presence is closely related to the maintenance of the pluripotency regulatory network and repression of mesendoderm lineages.
Article
Engineering, Biomedical
Lakshmi Kavitha Sthanam, Tanusri Roy, Sejal Patwardhan, Avi Shukla, Shipra Sharma, Pradip Shinde, Hanuman Tulasiram Kale, P. Chandra Shekar, Kiran Kondabagil, Shamik Sen
Summary: Stem cell differentiation is influenced by the interaction between cells and their extracellular matrix, with matrix metalloproteinases (MMPs) playing a role in this process. Differentiation of mouse embryonic stem cells (mESCs) on varying matrices led to changes in MMP expression and activity, affecting integrin expression, mechanotransduction pathways, and germ layer-specific markers. Inhibition of MMP activity altered the germ layer commitment of differentiating mESCs, demonstrating the importance of MMPs in regulating stem cell differentiation.
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Article
Pharmacology & Pharmacy
Michelle Vanessa Kamga Kapchoup, Juergen Hescheler, Filomain Nguemo
Summary: Hydroxychloroquine (HDQ) has been widely studied for its potential use in the treatment and prevention of COVID-19. This antimalarial drug has shown beneficial effects on stem cell proliferation and cardiomyocyte activity, but it can also be toxic at high concentrations. Identifying risk factors and understanding the cardiotoxic profile of HDQ is challenging.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Mohaddeseh Sadat Alavi, Hamid Reza Sadeghnia, Niloufar Nikpasand, Sajad Sahab Negah, Roghayeh Rashidi, Gholamreza Naseri, Ahmad Ghorbani
Summary: This study found that mephedrone exposure negatively affects the proliferation, differentiation, and apoptosis of rat embryonic neural stem/progenitor cells (NS/PC). Mephedrone at concentrations of 20-640 μM significantly reduced NS/PC proliferation, induced cell cycle arrest, and increased apoptosis and necrosis. Additionally, mephedrone decreased the expressions of DCX and NeuN neuronal markers. These findings suggest that mephedrone exposure during fetal or neonatal life may impair neurogenesis and subsequent brain development.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Mohaddeseh Sadat Alavi, Hamid Reza Sadeghnia, Niloufar Nikpasand, Sajad Sahab Negah, Roghayeh Rashidi, Gholamreza Naseri, Ahmad Ghorbani
Summary: This study investigated the effects of mephedrone exposure on the proliferation, differentiation, and apoptosis of rat embryonic NS/PC. The results showed that mephedrone significantly decreased the proliferation of NS/PC, induced cell cycle arrest, and increased the percentage of apoptotic and necrotic cells.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)