Article
Biotechnology & Applied Microbiology
Falguni Khan, Minlee Kim, Yonggyun Kim
Summary: A study found that RNA interference can be used to control the western flower thrips, a pest that damages hot peppers. Feeding specific double-stranded RNAs to the insects suppressed the expression of target genes and resulted in significant mortalities. Spraying liquid containing double-stranded RNAs also effectively controlled the population of the western flower thrips in a greenhouse.
Article
Multidisciplinary Sciences
Dandan Qian, Ye Cong, Runhao Wang, Quan Chen, Chuangye Yan, Deshun Gong
Summary: This study presents the cryo-electron microscopy structures of human SIDT2, which forms a dimer and acts as a transporter rather than a channel. Additionally, it exhibits ceramidase activity. These findings enhance our understanding of the structure-function relationships in the SID1 family proteins.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Daniel Endale Gebremichael, Zeraye Mehari Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi
Summary: The application of exogenous RNAi technology in managing plant pathogenic fungi has shown promising results in reducing disease severity, highlighting the great potential of RNAi-mediated control of fungal diseases in crops.
Article
Biochemistry & Molecular Biology
N. Hou, Z. Zhou, Y. Chen, J. Tian, Y. Zhang, Z. Liu
Summary: RNA interference is an important technology for gene functional research, and a method using bacteria expressing dsRNA for oral delivery was developed for gene functional study in the pond wolf spider. The results showed significant reduction of target gene mRNA abundance, demonstrating the effectiveness of this RNAi method in P. pseudoannulata.
INSECT MOLECULAR BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ruobing Guan, Dongdong Chu, Xinyi Han, Xuexia Miao, Haichao Li
Summary: RNA interference (RNAi) technology is widely used in medicine and agriculture, requiring large amounts of low-cost dsRNA. However, current microbial cell-based synthesis of dsRNA has low efficiency and yield, highlighting the need for the development of more efficient microbial dsRNA production systems in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Thais B. Rodrigues, Sambit K. Mishra, Krishnakumar Sridharan, Ethann R. Barnes, Andrei Alyokhin, Rich Tuttle, Wimalanathan Kokulapalan, David Garby, Nicholas J. Skizim, Yu-wen Tang, Brian Manley, Lorenzo Aulisa, Ronald D. Flannagan, Carole Cobb, Kenneth E. Narva
Summary: The Colorado potato beetle is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. Ledprona, a new biopesticide, has shown to be effective in controlling the beetle in laboratory assays. Its efficacy has been demonstrated in a whole plant greenhouse trial and it represents a new class of biopesticides for integrated pest management and insecticide resistance management programs against the CPB.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Rohit Sharma, Olivier Christiaens, Clauvis N. T. Taning, Guy Smagghe
Summary: This study assessed the potential of RNA interference (RNAi) technology to control the southern green stinkbug, Nezara viridula, through oral delivery of double-stranded RNA (dsRNA). The results showed that RNAi can be triggered by orally delivered dsRNA in N. viridula, but the rapid degradation of dsRNA in saliva and midgut suggests a need for further improvement in RNAi efficacy.
PEST MANAGEMENT SCIENCE
(2021)
Article
Physiology
Yayu Wang, Xiaoqiang Wang, Jingyuan Ge, Guiling Wang, Jiale Li
Summary: The regulatory element Tra-2 plays a critical role in sex formation in insects, with Hctra-2 identified in Hyriopsis cumingii showing potential involvement in regulating gonadal development. The expression of Hctra-2 is highest in the testes compared to ovaries, indicating a potential role in sex determination mechanisms in shellfish.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Agronomy
Kristopher Silver, Anastasia M. W. Cooper, Kun Yan Zhu
Summary: The article provides a comprehensive review of strategies for enhancing RNAi efficiency in insect pests, focusing on target gene selection and dsRNA delivery technologies. Recent research has focused on genome-wide screening for target gene selection and designing dsRNA constructs for maximal efficiency. In terms of dsRNA delivery, research has explored complexing or encapsulating dsRNA using various agents to enhance stability and uptake, as well as genetic engineering of microbes and plants for insect-specific dsRNA production.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Zhongzheng Ma, Yang Zheng, Zijian Chao, Hongtao Chen, Yunhui Zhang, Meizhen Yin, Jie Shen, Shuo Yan
Summary: A star polymer with positively-charged tertiary amine was synthesized and used to investigate the mechanism of gene delivery. The star polymer could protect gene fragments from degradation and promote cellular uptake and endocytosis. Transcriptome analysis revealed up-regulation of key genes involved in endocytosis. This study provides the first direct visualization of the detailed cellular process and mechanism of nanocarrier-mediated gene delivery.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Entomology
Peipei Liu, Wenping Zheng, Jiao Qiao, Ziniu Li, Zhurong Deng, Yimin Yuan, Hongyu Zhang
Summary: The study identified male-specific splicing patterns of the transformer gene in Zeugodacus tau, contributing to understanding sex determination mechanisms and gene regulation in embryonic development. Results were validated through quantitative real-time PCR, providing a reference for further research. High expression of certain genes at specific time points suggests their potential involvement in sex-specific splicing, requiring further functional analysis for confirmation.
Article
Physiology
Mosharrof Mondal, Megan Carver, Judith K. Brown
Summary: This study aimed to optimize RNA interference (RNAi) in young PoP adults and 3rd instars by screening through oral delivery of dsRNAs. The minimum effective concentration of dsRNA required for gene knockdown was determined to be 100 ng/μL for all three target genes. Gene silencing post-ingestion of 100 ng/μL dsRNA persisted for 3-5 days in adults and nymphs, with the 3rd instar showing the most robust RNAi response.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Agronomy
Mei Bai, Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Tong-Xian Liu, Hong-Gang Tian
Summary: Our study evaluated the effects of different dsRNA storage conditions on RNAi efficiency. The results showed that the dsRNA molecule was highly stable under typical cryopreservation temperatures and multiple freeze-thaw cycles, and there were no significant differences in RNAi efficiency. Surprisingly, dsRNA remained stable and induced an efficient RNAi response even under refrigerated storage conditions.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Chen-Hui Shen, Lin Jin, Kai-Yun Fu, Wen-Chao Guo, Guo-Qing Li
Summary: The study demonstrates the potential of using RNAi technology to control Leptinotarsa decemlineata, a serious pest attacking potatoes. Continuous ingestion of dsLdRan successfully silenced the target gene and inhibited larval growth, resulting in high mortality rates for larvae and adults.
PEST MANAGEMENT SCIENCE
(2022)
Review
Agronomy
Yuzhen Lu, Xinyue Deng, Qijun Zhu, Denghui Wu, Jielai Zhong, Liang Wen, Xiaoqiang Yu
Summary: RNA interference (RNAi) is a widely used method for silencing genes in various organisms and has been developed as a novel pest management strategy. Different delivery methods, such as microinjection, ingestion, soaking, oral and topical delivery, have been used for gene silencing in insect pests. Various genes involved in different processes can be targeted using RNAi, and RNAi-based pesticides have been developed in the form of genetically modified crops or as foliar sprays.