Article
Agronomy
Claudiane M. Rocha, Jaqueline F. Della Vechia, Patrice J. Savi, Celso Omoto, Daniel J. Andrade
Summary: Variability in susceptibility to spirodiclofen was high between Brevipalpus yothersi populations, with the frequency of resistant eggs ranging from 0.7% to 85.8% between populations. The resistance ratio of B. yothersi to spirodiclofen was low to moderate. Life table parameters indicated lower fitness in the resistant strain, with reduced survival rates and fecundity.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Chellappan Padmanabhan, Schyler Nunziata, M. Guillermo Leon, Yazmin Rivera, Vessela A. Mavrodieva, Mark K. Nakhla, Avijit Roy
Summary: Citrus leprosis (CiL) is an emerging viral disease of citrus in the Americas caused by Brevipalpus-transmitted viruses. The genetic diversity of viruses associated with CiL makes detection and diagnosis challenging, leading to the use of High-Throughput Sequencing (HTS) protocols. In this study, the HTS assay was used to detect and identify cileviruses infecting different host plants, leading to the discovery of a new strain of CiLV-C2 in hibiscus. These findings are important for citrus industry and further investigation is needed to understand the movement of the new strain from hibiscus to citrus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Agronomy
Melina Simeone, Claudio Gomez, Ana Bertalmio, Esperanza Ruiz, Claudia Hauteville, Laura Godoy Suarez, Blas Tito, Maria L. Garcia
Summary: Citrus psorosis virus (CPsV) is the main causal agent of citrus psorosis, and it is commonly diagnosed using biological indexing. This study showed that RT-qPCR is equivalent to biological indexing for diagnosing citrus psorosis, providing confidence in the quick diagnostic procedure for monitoring the health status of citrus trees.
Article
Microbiology
Camila Chabi-Jesus, Pedro L. Ramos-Gonzalez, Matheus Postclam-Barro, Rafaela Salgado Fontenele, Ricardo Harakava, Renato B. Bassanezi, Alecio S. Moreira, Elliot W. Kitajima, Arvind Varsani, Juliana Freitas-Astua
Summary: The study assessed the population and spatial dynamic parameters of citrus leprosis virus C from samples collected between 1932 and 2020. Three major lineages of the virus were identified with high nucleotide sequence identity of around 85% and signs of inter-clade recombination events. The high prevalence of CiLV-C in the Brazilian citrus belt is likely due to intensive connectivity between orchards, posing a potential risk for pathogen saturation in the region.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Shun-Min Yao, Meng-Ling Wu, Ting-Hsuan Hung
Summary: A multiplex RT-PCR assay was developed in this study to simultaneously detect citrus Huanglongbing, citrus tristeza virus, citrus tatter leaf virus, and citrus exocortis viroid. Compared with previous simplex assays, the multiplex RT-PCR showed better performance in detecting field samples and can be used for routine disease surveying and producing pathogen-free citrus plants.
Article
Microbiology
Dilip Kumar Ghosh, Amol Kokane, Sunil Kokane, Krishanu Mukherjee, Jigme Tenzin, Datta Surwase, Dhanshree Deshmukh, Mrugendra Gubyad, Kajal Kumar Biswas
Summary: This study detected a high incidence of citrus tristeza virus (CTV) infection in Mandarin orange samples from Bhutan using reverse transcription PCR (RT-PCR). Genetic variability among Bhutanese CTV variants was analyzed by sequencing four independent genomic regions and comparing with GenBank deposited sequences. The study identified unexpected diversity among the Bhutanese CTV variants and also discovered a resistant-breaking variant. The findings contribute to the understanding of CTV diversity and provide insights into pathogenicity assessments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Zhen Liu, Zhenfei Dong, Binhui Zhan, Shifang Li
Summary: The first identification and characterization of citrus concave gum-associated virus (CCGaV) infecting apples in China was achieved through high throughput sequencing, and a rapid and sensitive detection technique was developed.
Article
Biotechnology & Applied Microbiology
Hyuna Park, Wonjong Jung, Hyeongseok Jang, Kak Namkoong, Kwon-Young Choi
Summary: This study developed a simple and rapid thermolysis method that can bypass the need for viral RNA extraction and purification. The method integrates thermolysis, RT, and digital PCR into a single chip for large-scale viral analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Genetics & Heredity
Amjad Ali, Ummad ud Din Umar, Syed Atif Hasan Naqvi, Muhammad Taimoor Shakeel, Muhammad Nouman Tahir, Muhammad Fahad Khan, Muhammad Tanveer Altaf, Fatih Olmez, Abdelfattah A. Dababat, Zia ul Haq, Muhammad Azhar Nadeem, Rustu Hatipoglu, Faheem Shehzad Baloch, Yong Suk Chung
Summary: Citrus viroid infection poses a serious threat to the citrus industry due to its rapid spread and efficient systemic movement within host plants. This study investigated the distribution and molecular characterization of Citrus bent leaf viroid (CBLVd) and its variants in different citrus cultivars, highlighting the need for effective management and quarantine measures to control its spread.
FRONTIERS IN GENETICS
(2023)
Article
Agronomy
Patricia Magnaboschi Hesketh, Mario Eidi Sato
Summary: This study evaluated the susceptibility of different populations of E. concordis and E. citrifolius from citrus groves in São Paulo state, Brazil, to acaricides. The results showed that E. citrifolius populations had resistance to abamectin and chlorfenapyr, and low susceptibility to fenpropathrin. One population of E. citrifolius exhibited high predation capacity on B. yothersi larvae.
Article
Microbiology
Eduardo Henrique Goulin, Thiago Antonio de Lima, Paulo Jose Camargo dos Santos, Marcos Antonio Machado
Summary: Citrus crop is significant globally, with Brazil being the largest producer of sweet oranges. Fungal diseases and fungicide resistance are challenges in citrus cultivation. New technologies like RNA interference offer alternatives to chemical control methods.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Entomology
Thais Elise Sinico, Maria Andreia Nunes, Elliot Watanabe Kitajima, Bruna Aparecida Cunha, Valdenice Moreira Novelli
Summary: This study provided a descriptive analysis of the embryonic development of the economically important mite vector B. yothersi, revealing its embryological characteristics and biological information.
Article
Microbiology
Pedro L. Ramos-Gonzalez, Camila Chabi-Jesus, Aline D. Tassi, Renata Faier Calegario, Ricardo Harakava, Claudia F. Nome, Elliot W. Kitajima, Juliana Freitas-Astua
Summary: In this study, molecular and biological features of three new viruses of the family Kitaviridae were described through various analyses. These viruses are transmitted by Brevipalpus mites, which infect plants. The study also identified the Brevipalpus mite species associated with these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Aline Daniele Tassi, Pedro Luis Ramos-Gonzalez, Thais Elise Sinico, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: This study investigated the transmission mechanism of the plant virus CiLV-C by Brevipalpus mites. Through direct observation using an electron microscope, the researchers proposed the hypothesis of viral movement through intercellular spaces in the mites and described the presence of viral particles in the mite's body. The study also suggested potential genes that may control the circulation of the virus within the mite.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Plant Sciences
Pedro Luis Ramos-Gonzalez, Hideki Kondo, Sergey Morozov, Nikolaos Vasilakis, Arvind Varsani, Mengji Cao, Juliana Freitas-Astua
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Peter J. Walker, Juliana Freitas-Astua, Nicolas Bejerman, Kim R. Blasdell, Rachel Breyta, Ralf G. Dietzgen, Anthony R. Fooks, Hideki Kondo, Gael Kurath, Ivan Kuzmin, Pedro Luis Ramos-Gonzalez, Mang Shi, David M. Stone, Robert B. Tesh, Noel Tordo, Nikos Vasilakis, Anna E. Whitfield
Summary: The family Rhabdoviridae consists of viruses with negative-sense RNA genomes and can infect a wide range of hosts including plants, animals, and arthropods. They are important pathogens for humans, livestock, and agricultural crops.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Virology
Jens H. Kuhn, Scott Adkins, Sergey Alkhovsky, Tatjana Avsic-Zupanc, Maria A. Ayllon, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Martina Bandte, Martin Beer, Nicolas Bejerman, Eric Bergeron, Nadine Biedenkopf, Laurent Bigarre, Carol D. Blair, Kim R. Blasdell, Steven B. Bradfute, Thomas Briese, Paul A. Brown, Remy Bruggmann, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Buettner, Charles H. Calisher, Thierry Candresse, Jeremy Carson, Inmaculada Casas, Kartik Chandran, Remi N. Charrel, Yuya Chiaki, Anya Crane, Mark Crane, Laurent Dacheux, Elena Dal Bo, Juan Carlos de la Torre, Xavier de Lamballerie, William M. de Souza, Rik L. de Swart, Nolwenn M. Dheilly, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, J. Felix Drexler, W. Paul Duprex, Ralf Duerrwald, Andrew J. Easton, Toufc Elbeaino, Koray Ergunay, Guozhong Feng, Claudette Feuvrier, Andrew E. Firth, Anthony R. Fooks, Pierre B. H. Formenty, Juliana Freitas-Astua, Selma Gago-Zachert, Maria Laura Garcia, Adolfo Garcia-Sastre, Aura R. Garrison, Scott E. Godwin, Jean-Paul J. Gonzalez, Joelle Gouy de Bellocq, Anthony Grifths, Martin H. Groschup, Stephan Gunther, John Hammond, Jussi Hepojoki, Melanie M. Hierweger, Seiji Hongo, Masayuki Horie, Hidenori Horikawa, Holly R. Hughes, Adam J. Hume, Timothy H. Hyndman, Daohong Jiang, Gilda B. Jonson, Sandra Junglen, Fujio Kadono, David G. Karlin, Boris Klempa, Jonas Klingstrom, Michel C. Koch, Hideki Kondo, Eugene Koonin, Jarmila Krasova, Mart Krupovic, Kenji Kubota, Ivan Kuzmin, Lies Laenen, Amy J. Lambert, Jianrong Li, Jun-Min Li, Francois Liefrig, Igor S. Lukashevich, Dongsheng Luo, Piet Maes, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, John W. McCauley, Ali Mirazimi, Peter G. Mohr, Nick J. G. Moody, Yasuaki Morita, Richard N. Morrison, Elke Muhlberger, Rayapati Naidu, Tomohide Natsuaki, Jose A. Navarro, Yutaro Neriya, Sergey Netesov, Gabriele Neumann, Norbert Nowotny, Francisco M. Ochoa-Corona, Gustavo Palacios, Laurane Pallandre, Vicente Pallas, Anna Papa, Sofa Paraskevopoulou, Colin R. Parrish, Alex Pauvolid-Correa, Janusz T. Paweska, Daniel R. Perez, Florian Pfaf, Richard K. Plemper, Thomas S. Postler, Francoise Pozet, Sheli R. Radoshitzky, Pedro L. Ramos-Gonzalez, Marius Rehanek, Renato O. Resende, Carina A. Reyes, Victor Romanowski, Dennis Rubbenstroth, Luisa Rubino, Artemis Rumbou, Jonathan A. Runstadler, Melanie Rupp, Sead Sabanadzovic, Takahide Sasaya, Heike Schmidt-Posthaus, Martin Schwemmle, Torsten Seuberlich, Stephen R. Sharpe, Mang Shi, Manuela Sironi, Sophie Smither, Jin-Won Song, Kirsten M. Spann, Jessica R. Spengler, Mark D. Stenglein, Ayato Takada, Robert B. Tesh, Jana Tesikova, Natalie J. Thornburg, Nicole D. Tischler, Yasuhiro Tomitaka, Keizo Tomonaga, Noel Tordo, Kenta Tsunekawa, Massimo Turina, Ioannis E. Tzanetakis, Anna Maria Vaira, Bernadette van den Hoogen, Bert Vanmechelen, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Anna E. Whitfeld, John Williams, Yuri Wolf, Junki Yamasaki, Hironobu Yanagisawa, Gongyin Ye, Yong-Zhen Zhang, Arnfnn Lodden Okland
Summary: This article presents the updated taxonomy of Negarnaviricota, including the addition of new families, genera, and species, renaming and moving of species, and correction of misspelled names as accepted by the International Committee on Taxonomy of Viruses (ICTV).
ARCHIVES OF VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Matheus Luis Docema, Tatiana de Souza Moraes, Lisia Borges Attilio, Joao Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Ricardo Harakava, Juliana Freitas-Astua, Jose Belasque Junior, Ricardo Antunes Azevedo, Salete Aparecida Gaziola, Francisco de Assis Alves Mourao Filho
Summary: Currently, there are no resistant sweet orange cultivars to citrus canker. Transgenic plants expressing antimicrobial peptides and resistance genes from tolerant cultivars were evaluated for resistance to Xanthomonas citri subsp. citri (Xcc). Transgenic plants showed reduced incidence and severity of citrus canker, as well as lower Xcc titers. Higher superoxide dismutase activity in transgenic plants led to increased hydrogen peroxide production. Callose deposition, an important plant defense response, was observed in transgenic plants with lower Xcc populations.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Review
Plant Sciences
Pedro Luis Ramos-Gonzalez, Gabriella Dias Arena, Aline Daniele Tassi, Camila Chabi-Jesus, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: Kitaviridae is a family of plant-infecting viruses that have unique localized infections and show deficient movement. They are transmitted by mites and infect a wide range of host plants, causing economic damage to crops.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Silvia de Oliveira Dorta, Lisia Borges Attilio, Odimar Zanuzo Zanardi, Joao Roberto Spotti Lopes, Marcos Antonio Machado, Juliana Freitas-Astua
Summary: The Asian citrus psyllid (ACP) is the vector of the devastating disease huanglongbing (HLB) in citrus. Previous research has shown the potential of using Bt strains containing cry/cyt genes to control ACP nymphs. This study attempted to produce transgenic sweet orange plants expressing the cry11A gene from Bt, and confirmed the presence and copy number of the transgene using PCR and Southern blot analyses. Greenhouse mortality assays showed that the tested events had effects ranging from 22% to 43% on D. citri nymphs after a five-day exposure period. This is the first report of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Camila Chabi-Jesus, Pedro Luis Ramos-Gonzalez, Aline Daniele Tassi, Laura Rossetto Pereira, Marines Bastianel, Douglas Lau, Maria Cristina Canale, Ricardo Harakava, Valdenice Moreira Novelli, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: Citrus leprosis (CL) is a viral disease that seriously affects Brazilian citriculture. In this study, a new virus called citrus bright spot virus (CiBSV) was discovered in sweet orange trees affected by CL in small orchards in Southern Brazil. Sequence analysis revealed that CiBSV belongs to the genus Dichorhavirus and clusters with citrus leprosis virus N, which is transmitted by Brevipalpus phoenicis. Brevipalpus papayensis and B. azores were found in CiBSV-infected citrus plants, with B. azores being the only vector capable of transmitting the virus to Arabidopsis plants.
Article
Plant Sciences
Gabriella D. Arena, Pedro L. Ramos-Gonzalez, Aline D. Tassi, Marcos A. Machado, Juliana Freitas-Astua
Summary: This study developed two TaqMan RT-qPCR assays for detecting and quantifying Citrus leprosis virus C (CiLV-C) lineage SJP, which is prevalent in the Brazilian citrus belt and the world's main sweet orange production area. The assays targeted two specific genes, p29 and RdRp, and were proven to be efficient and sensitive. By quantifying the target genes in different tissues and organisms, the study revealed the distribution and accumulation of CiLV-C molecules, the higher quantities in sweet orange fruits compared to stems and leaves, as well as the progressive increase during the early stages of infection. These findings contribute to a better understanding of the epidemiology and virus-vector interaction of citrus leprosis.
TROPICAL PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Maria Andreia Nunes, Leticia Maria Ferreira, Luana Aparecida Rogerio, Elliot Watanabe Kitajima, Valdenice Moreira Novelli
Summary: This study reports for the first time the infection of succulent plants, panda plant and bear's paw, with CoRSV transmitted by viruliferous B. papayensis females. Symptoms include chlorotic and necrotic spots on leaves, and the presence of the virus was confirmed using molecular detection and electron microscopy.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Jessika Angelotti-Mendonca, Perla Novais de Oliveira, Nathalia Felipe Ansante, Liliane Cristina Liborio Stipp, Juliana Freitas-Astua, Fernando Manuel Matias Hurtado, Jose Belasque, Francisco de Assis Alves Mourao Filho
Summary: Researchers investigated the role of the CsEDS5 gene in the resistance of transgenic plants by transforming it into Micro-Tom tomato plants. They found that some transgenic plants exhibited higher resistance to X. vesicatoria, which could be significant in mitigating the impact of citrus canker.
TROPICAL PLANT PATHOLOGY
(2022)
