Journal
EXPERIMENTAL AND APPLIED ACAROLOGY
Volume 50, Issue 2, Pages 163-168Publisher
SPRINGER
DOI: 10.1007/s10493-009-9293-8
Keywords
Rhipicephalus sanguineus; Ehrlichia chaffeensis; Ehrlichia canis; Zoonosis; Cameroon
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Funding
- Forgarty International Center [D43TW00903]
- Sealy Center for Vaccine Development
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Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.
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