Article
Genetics & Heredity
Dandan Li, Xiaomei Li, Xintong Liu, Zhao Zhang
Summary: This study conducted a comprehensive genome-wide analysis of the bZIP gene family (RcbZIPs) in rose, revealing their phylogenetic relationships, gene structure, chromosome localizations, gene duplication, and potential functions. The expression analysis showed significant differential expression of 16 RcbZIPs in response to Botrytis cinerea infection, suggesting their candidate role in regulating rose's response to gray mold infection. Virus-induced gene silencing (VIGS) and overexpression (OE) studies demonstrated the involvement of RcbZIP17 in rose's resistance to gray mold. This study provides valuable information for further investigation into the function of the bZIP gene family in rose.
Article
Microbiology
Gabriel Perez-Lara, Tomas C. Moyano, Andrea Vega, Luis F. Larrondo, Ruben Polanco, Jose M. Alvarez, Daniel Aguayo, Paulo Canessa
Summary: To comprehensively analyze gene expression in the phytopathogenic fungus Botrytis cinerea, a web-based gene expression browser called BEB was developed. This computationally inexpensive application provides publication-ready heatmaps of transcript levels and allows gene expression clustering and visualization options, along with the ability to download gene expression values for further exploration. The implementation of BEB is transferable to other organisms and allows for comparative transcriptomics and candidate gene identification.
Article
Microbiology
Polina C. Tsalgatidou, Eirini-Evangelia Thomloudi, Eirini Baira, Konstantinos Papadimitriou, Aggeliki Skagia, Anastasia Venieraki, Panagiotis Katinakis
Summary: The endophytic strain Cal.l.30, isolated from the medicinal plant Calendula officinalis, shows strong potential against the postharvest fungal pathogen Botrytis cinerea. It synthesizes and secretes secondary metabolites with antimicrobial activity, providing new perspectives on the exploitation of novel Bacillus sp. as a biocontrol agent.
Article
Microbiology
Zhao Wei, Dan Shu, Qun Sun, Dong-bo Chen, Zhe-min Li, Di Luo, Jie Yang, Hong Tan
Summary: This study demonstrates the important role of BcLAE1 in the regulation of ABA biosynthesis in Botrytis cinerea, and further reveals its involvement in secondary metabolism and epigenetic regulation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Consuelo Olivares-Yanez, Evelyn Sanchez, Gabriel Perez-Lara, Aldo Seguel, Pamela Y. Camejo, Luis F. Larrondo, Elena A. Vidal, Paulo Canessa
Summary: By generating manually curated transcription factor datasets and conducting network analysis, we studied the transcriptional gene regulatory networks of Botrytis and Trichoderma, revealing significant differences in their transcriptomes under different biological paradigms and distinctive gene expression changes due to confrontation.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Forestry
Jun Sun, Yue Zhang, Zeyang Zheng, Jiachen Hao, Lijing Chen
Summary: Botrytis cinerea is a pathogenic fungus that causes necrosis in plants and is highly destructive to hazelnuts. The plant's defense mechanism against this fungus involves complex changes at molecular, biochemical, and physiological levels. A comparative transcriptome profiling study revealed moderate changes in the defense strategies of resistant and susceptible hazelnut varieties after three days of B. cinerea infection. The study identified differentially expressed disease-resistant genes in the resistant variety, suggesting their crucial role in inducing innate resistance.
Article
Forestry
Lin Rui, Wei-Liang Kong, Xiao-Rui Sun, Xiao-Qin Wu
Summary: In May 2019, leaf spots were observed on Aucuba japonica var. variegata in Nanjing City, Jiangsu Province, China. The fungus isolated from the lesions was identified as Botrytis cinerea based on its morphological characteristics and phylogenetic analysis. This is the first report of B. cinerea causing leaf spots on A. japonica var. variegata.
Article
Agronomy
Chuangju Ma, Jinsong Xiong, Morong Liang, Xiaoyu Liu, Xiaodong Lai, Yibo Bai, Zongming Cheng
Summary: The study revealed that FvWRKY50 acts as a positive regulator in mediating resistance against B. cinerea by regulating some JA pathway and defense-related genes.
Article
Agriculture, Multidisciplinary
Zhexin Li, Shuai Yang, Yijia Ma, Yuan Sui, Haitao Xing, Wenli Zhang, Qinhong Liao, Yusong Jiang
Summary: This study found that Ac-miR160d plays a positive role in regulating kiwifruit resistance to B. cinerea. Kiwifruit with overexpressed Ac-miR160d showed increased resistance to B. cinerea, along with increased antioxidant enzyme activity and phytohormone content. RNA-seq analysis revealed the metabolic pathways regulated by Ac-miR160d, providing valuable gene resources for molecular breeding of kiwifruit resistance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Lijiahong Geng, Yalun Fu, Xiaohua Peng, Zhengkun Yang, Mengke Zhang, Zhiyu Song, Ning Guo, Shuangchen Chen, Junliang Chen, Bing Bai, Airong Liu, Golam Jalal Ahammed
Summary: This study investigated the effects of Trichoderma harzianum on Botrytis cinerea and its underlying mechanisms. The results showed that Trichoderma harzianum enhanced tomato resistance to gray mold disease by regulating cellular redox, secondary metabolites, and stomatal movement.
BIOLOGICAL CONTROL
(2022)
Article
Biotechnology & Applied Microbiology
Joshua G. Philips, Elena Martin-Avila, Andrea V. Robold
Summary: This review provides updated evidence on the likelihood, factors, and barriers for horizontal gene transfer in genetically modified plants, and presents the legislation and frameworks followed by the Australian Gene Technology Regulator in considering the risks posed by horizontal gene transfer.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Moira Giovannoni, Damiano Lironi, Lucia Marti, Chiara Paparella, Valeria Vecchi, Andrea A. Gust, Giulia De Lorenzo, Thorsten Nurnberger, Simone Ferrari
Summary: In Arabidopsis thaliana, the proteins CERK1, LYK2, and LYK5 are necessary for chitin-induced resistance to the pathogen B. cinerea, with LYK2 contributing to callose deposition but not essential for chitin perception. LYK2 is also required for enhanced resistance to other pathogens induced by flagellin and for priming of defense gene expression during fungal infection, ensuring robust resistance to subsequent pathogen infections.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Microbiology
Na Liu, Shanyue Zhou, Baohua Li, Weichao Ren
Summary: In this study, the biological function of the autophagy-related protein Atg6 in B. cinerea was identified and characterized. Targeted deletion of the BcATG6 gene resulted in blockage of autophagy and various phenotypic defects, which were restored by targeted gene complementation. These results suggest that BcAtg6 plays important roles in regulating various cellular processes in B. cinerea.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Wenjun Zhu, Huange Dong, Ran Xu, Jingmao You, Da-zhong Yan, Chao Xiong, Jing Wu, Kai Bi
Summary: Cell death-inducing proteins (CDIPs) play crucial roles in the infection of the phytopathogen Botrytis cinerea. The secreted protein BcCDI1 can induce necrosis in tobacco leaves and activate plant defense. BcCDI1 is possibly recognized by plant receptors and triggers plant cell death.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yuwen Li, Suhua Li, Ran Du, Jiaojiao Wang, Haiou Li, Daoxin Xie, Jianbin Yan
Summary: Research showed that application of isoleucine can enhance plant resistance to Botrytis cinerea in Arabidopsis, dependent on JA signaling. The increase of Ile content in leaves results in enhanced resistance to B. cinerea infection. Exogenous Ile application also moderately enhances resistance to B. cinerea in various horticultural plant species, offering a practical strategy for disease control in agriculture.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Food Science & Technology
Temoor Ahmed, Muhammad Noman, Muhammad Rizwan, Shafaqat Ali, Muhammad Shafiq Shahid, Bin Li
Summary: This article focuses on the toxicity of heavy metals in paddy soils and their potential health risks to humans, as well as providing a critical outlook on the recent advances and future perspectives of nanoremediation strategies.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Environmental Sciences
A. Abbas, T. Ahmad, S. Hussain, M. Noman, T. Shahzad, A. Iftikhar, Cheema, M. Ijaz, M. Tahir, G. Gohari, B. Li, F. Mahmood, M. Shahid
Summary: Untreated wastewater discharged from textile units contains toxic dyes, posing carcinogenic and mutagenic hazards. In this study, biogenic zinc oxide nanoparticles immobilized on calcium alginate beads were found to have promising photocatalytic potential for degrading dyes and treating textile effluents.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hubiao Jiang, Luqiong Lv, Temoor Ahmed, Shaomin Jin, Muhammad Shahid, Muhammad Noman, Hosam-Eldin Hussein Osman, Yanli Wang, Guochang Sun, Xuqing Li, Bin Li
Summary: This study found that metal oxide nanoparticles can reduce the incidence of tomato bacterial wilt and alter the composition and structure of the rhizosphere bacterial community. Among them, the application of CuO nanoparticles significantly affected the abundance of beneficial Streptomyces bacteria. This research provides evidence and strategies for using metal oxide nanoparticles to prevent and control soil-borne disease tomato bacterial wilt.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Temoor Ahmed, Muhammad Noman, Muhammad Rizwan, Shafaqat Ali, Usman Ijaz, Muhammad Mudassir Nazir, Haifa Abdulaziz Sakit ALHaithloul, Suliman Mohammed Alghanem, Awatif M. Abdulmajeed, Bin Li
Summary: This study demonstrated that the synergistic application of biogenic nanoparticles and plant growth promoting rhizobacteria can effectively mitigate the negative impacts of arsenic contamination in agricultural soils, showing good biocompatibility. This approach not only improves wheat plant morphology and nutrient content, and maintains ionic balance, but also significantly reduces arsenic translocation in plants.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Agronomy
Haiying Ren, Hongyan Wang, Qi Wang, Xingjiang Qi, Shuwen Zhang, Zheping Yu, Munazza Ijaz, Muchen Zhang, Temoor Ahmed, Mohamed El-Sharnouby, Mohamed M. Hassan, Zhenshuo Wang, Bin Li
Summary: The application of prochloraz improved tree vigor and fruit quality of decline-diseased bayberry. The beneficial effect of prochloraz on plants and fruits may be related to its influence on rhizosphere soil properties and microbiota. Prochloraz significantly affected the abundance, diversity, and composition of rhizosphere soil microbiota, particularly bacteria. It also affected the metabolic pathways and contents of various metabolites in the plant.
Article
Biotechnology & Applied Microbiology
Peng Zhang, Jiekai Ye, Lixia Huang, Xiaozhang Ying, Bo Zhu, Yanghui Jin, Jun Fei, Shiyuan Shi, Qi Zheng
Summary: The study compares the clinical efficacy of CBT screw fixation and PS fixation in lumbar tuberculosis patients. Both approaches achieved satisfactory outcomes, with CBT showing better improvement in postoperative incision pain VAS scores.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Agronomy
Xuqing Li, Dingyi Li, Qiujun Lu, Daoze Wang, Xiaoxu Ren, Luqiong Lv, Temoor Ahmed, Jianli Yan, Bin Li
Summary: This study investigated the effects of commercial organic fertilizer, sheep manure, mushroom residue, and chemical compound fertilizer on newly reclaimed land. The results showed that these amendments did not significantly change soil pH, but significantly increased organic matter content, suggesting their potential for improving soil quality. However, they had different effects on sweet potato growth, nutrient elements, and microbial community, depending on the type and concentration of fertilizer, application time, as well as plant and soil parameters.
Article
Plant Sciences
Xuqing Li, Qiujun Lu, Dingyi Li, Daoze Wang, Xiaoxu Ren, Jianli Yan, Temoor Ahmed, Bin Li
Summary: This study evaluated the effects of different microbial fertilizers on immature soil and found significant differences in soil properties and microbial community structure between seaweed microbial fertilizer (SMF) and commercial chemical compound fertilizers. The main variables affecting the microbial communities and soil properties were found to be exchangeable Ca, organic matter content, total N, and available P.
Article
Plant Sciences
Xuqing Li, Qiujun Lu, Dingyi Li, Daoze Wang, Xiaoxu Ren, Jianli Yan, Temoor Ahmed, Bin Li
Summary: Due to urbanization and industrialization, cultivated land resources have been occupied and new reclamation land can expand the supply of usable land. Organic fertilizers are effective amendments in improving the quality of immature soil. The use of commercial organic fertilizers, especially sheep manure organic fertilizer, can be beneficial for new reclamation land.
Article
Biochemistry & Molecular Biology
Haiying Ren, Mohammad Shafiqul Islam, Hongyan Wang, Hao Guo, Zhenshuo Wang, Xingjiang Qi, Shuwen Zhang, Junning Guo, Qi Wang, Bin Li
Summary: The application of humic acid is effective in improving the vigor and fruit quality of diseased bayberry trees and increasing the diversity of microbial communities in the rhizosphere soil. Available nitrogen, phosphorus, potassium, and calcium have a significant impact on the composition of bacterial and fungal communities in the bayberry rhizosphere soil.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Virology
Temoor Ahmed, Bin Li
Article
Plant Sciences
Changxin Li, Gang Li, Xingjiang Qi, Zheping Yu, Yasmine Abdallah, Solabomi Olaitan Ogunyemi, Shuwen Zhang, Haiying Ren, Mohamed Mohany, Salim S. Al-Rejaie, Bin Li, Erming Liu
Summary: This study explored the mechanism of the beneficial effect of accompanying ryegrass on the fruit quality of bayberry. The findings showed that planting ryegrass significantly improved the fruit quality of bayberry, and caused changes in the physical and chemical properties, microbiota, and secondary metabolites of the bayberry rhizosphere soils.
Review
Plant Sciences
Temoor Ahmed, Muhammad Noman, Yetong Qi, Muhammad Shahid, Sabir Hussain, Hafiza Ayesha Masood, Lihui Xu, Hayssam M. Ali, Sally Negm, Attalla F. El-Kott, Yanlai Yao, Xingjiang Qi, Bin Li
Summary: Microbial compost plays a crucial role in improving soil fertility and agricultural productivity by enhancing nutrient acquisition and controlling pathogens, contributing to sustainable agriculture.
Review
Plant Sciences
Munazza Ijaz, Fahad Khan, Haitham E. M. Zaki, Muhammad Munem Khan, Khlode S. A. Radwan, Yugen Jiang, Jiahui Qian, Temoor Ahmed, Muhammad Shafiq Shahid, Jinyan Luo, Bin Li
Summary: Targeted genome editing technologies, such as CRISPR/Cas, have become crucial tools in phytopathology research. They offer flexibility, simplicity, and high efficiency in genome editing and have been used successfully in various applications. This review provides an overview of different CRISPR/Cas systems and their applications in targeting important genes involved in plant-pathogen interactions, improving pathogen resistance, and studying plant defense mechanisms.
Editorial Material
Plant Sciences
Temoor Ahmed, Muhammad Noman, Muhammad Shahid, Amir Hameed, Bin Li