In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre

A triterpene glycoside (TG) fraction isolated and purified fromethanolic extract of Gymnema sylvestre (EEGS) was investigated for blood glucose control benefit using in vitro methods. The HPLC purified active fraction TG was characterized using FTIR, LC-MS, and NMR. The purified fraction (TG) exhibited effective inhibition of yeast alpha-glucosidase, sucrase, maltase, and pancreatic.. amylase with IC50 values 3.16 +/- 0.05 mu g/mL, 74.07 +/- 0.51, 5.69 +/- 0.02, and 1.17 +/- 0.24 mu g/mL, respectively, compared to control. TG was characterized to be a mixture of triterpene glycosides: gymnemic acids I, IV, and VII and gymnemagenin. In vitro studies were performed usingmouse pancreatic beta-cell lines (MIN6). TGdid not exhibit any toxic effects on beta-cell viability and showedprotection against H2O2 induced ROS generation. There was up to 1.34- fold increase in glucose stimulated insulin secretion (p<0.05) in a dose-dependent manner relative to standard antidiabetic drug glibenclamide. Also, there was further one-fold enhancement in the expression of GLUT2 compared to commercial standard DAG (deacylgymnemic acid). Thus, the present study highlights the effective isolation and therapeutic potential of TG, making it a functional food ingredient and a safe nutraceutical candidate for management of diabetes.