Article
Biology
Tzitzitlini Alejandre-Garcia, Samuel Kim, Jesus Perez-Ortega, Rafael Yuste
Summary: Neuronal ensembles are coactive groups of cortical neurons that play a role in perception and behavior. In this study, we replicated an in vivo optogenetic protocol in brain slices to investigate the mechanisms involved in ensemble formation. We found that coactivated neurons showed increased correlated activity and biphasic changes in presynaptic plasticity. Furthermore, stimulation induced significant increases in spontaneous EPSPs and neuronal excitability.
Article
Biochemistry & Molecular Biology
Stefania Scalise, Clara Zannino, Valeria Lucchino, Michela Lo Conte, Luana Scaramuzzino, Pierangelo Cifelli, Tiziano D'Andrea, Katiuscia Martinello, Sergio Fucile, Eleonora Palma, Antonio Gambardella, Gabriele Ruffolo, Giovanni Cuda, Elvira Immacolata Parrotta
Summary: This study investigates the effects of a SCN1A gene mutation on neuronal maturation and excitability using induced pluripotent stem cell-derived neurons. The results provide evidence for intrinsic cellular immaturity and support the importance of mutant Na(V)1.1 in the development of febrile seizures. The findings also suggest that diseased neurons can be a powerful tool for personalized therapy and drug screening for epileptic disorders.
Article
Biochemistry & Molecular Biology
Megan Allen, Ben S. Huang, Michael J. Notaras, Aiman Lodhi, Estibaliz Barrio-Alonso, Pablo J. Lituma, Paul Wolujewicz, Jonathan Witztum, Francesco Longo, Maoshan Chen, David W. Greening, Eric Klann, M. Elizabeth Ross, Conor Liston, Dilek Colak
Summary: This study reveals that astrocytes derived from individuals with autism spectrum disorder (ASD) have the potential to induce repetitive behavior and cognitive deficits. Moreover, astrocyte abnormalities may play a role in the pathogenesis of ASD.
MOLECULAR PSYCHIATRY
(2022)
Article
Neurosciences
Han-Qing Pan, Xiao-Xuan Liu, Ye He, Jin Zhou, Cai-Zhi Liao, Wen-Jie You, Si-Ying Jiang, Xia Qin, Wen-Bing Chen, Er-Kang Fei, Wen-Hua Zhang, Bing-Xing Pan
Summary: This study reveals the unconventional and permissive role of extrasynaptic GABA(A) receptors in fear extinction through a route relying on nonsynaptic plasticity. The expression and function of GABA(A)(δ)R in the medial prefrontal cortex (mPFC) are positively correlated with fear extinction, and knockdown of GABA(A)(δ)R in the mPFC impairs fear extinction. Mechanistically, GABA(A)(δ)R enables the extinction-evoked plastic regulation of neuronal excitability in mPFC projection neurons.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Roberto Colangeli, Maria Morena, Allison Werner, Roger J. Thompson, Mario van der Stelt, Quentin J. Pittman, Matthew N. Hill, Campbell Teskey
Summary: Repeated seizures result in persistent alteration of endocannabinoid (eCB) signaling, particularly a deficiency in anandamide signaling in the basolateral amygdala (BLA), leading to abnormal synaptic function and emotional behavior. This study found that repeated seizures reduced tonic and phasic eCB-mediated retrograde signaling in GABA transmission. These effects were not due to changes in CB1 receptor sensitivity or altered baseline 2-AG signaling, but rather impaired stimulus-dependent 2-AG synthesis/release.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Clinical Neurology
Florian Studer, Guillaume Jarre, Benoit Pouyatos, Christian Nemoz, Elke Brauer-Krisch, Clemence Muzelle, Raphael Serduc, Christophe Heinrich, Antoine Depaulis
Summary: In this study, the researchers used a genetic model of absence epilepsy in rats to investigate the role of structural connectivity of cortical neurons in the generation of absence seizures. The results showed that increased structural connectivity contributes to the increased neuronal synchronization associated with absence seizures. This study provides strong evidence that aberrant structural connectivity patterns of cortical neurons are critical pathological substrates for increased neuronal synchronization and the generation of absence seizures.
Article
Biology
V. Korzhova, P. Marinkovic, J. Rudan Njavro, P. M. Goltstein, F. Sun, S. Tahirovic, J. Herms, S. Liebscher
Summary: The study utilized in vivo two-photon calcium imaging to record the activity of cortical neurons in awake APPPS1 transgenic mice, revealing that persistent single cell aberrant neuronal activity is the cause of neuronal network pathology in cerebral amyloidosis models, which may have diagnostic and therapeutic implications for Alzheimer's disease (AD).
COMMUNICATIONS BIOLOGY
(2021)
Article
Neurosciences
Gary W. Mathern, Edward H. Bertram
Summary: This study found that neuronal loss associated with limbic epilepsy precedes the onset of seizures and is not a consequence of recurrent seizures. However, intermittent seizures do cause structural changes in the brain, the functional consequences of which are unclear.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Grigori Y. Rychkov, Zeeshan Shaukat, Chiao Xin Lim, Rashid Hussain, Ben J. Roberts, Claudia M. Bonardi, Guido Rubboli, Brandon F. Meaney, Robyn Whitney, Rikke S. Moller, Michael G. Ricos, Leanne M. Dibbens
Summary: The effects of various KCNT1 mutations on the severity of neurological disorders were investigated, and a positive correlation was found between the severity of the disorder and the KCNT1 channel open probability at resting membrane potential. This suggests that gain of function KCNT1 mutations cause epilepsy by increasing resting potassium conductance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Edward M. Merricks, Elliot H. Smith, Ronald G. Emerson, Lisa M. Bateman, Guy M. McKhann, Robert R. Goodman, Sameer A. Sheth, Bradley Greger, Paul A. House, Andrew J. Trevelyan, Catherine A. Schevon
Summary: By developing a novel template-matching-based spike sorting method, researchers successfully identified 1239 single neurons in patients with epilepsy, observing waveform alterations in tissue invaded by seizures and stable waveforms in penumbral regions. All neurons returned to preictal waveforms after seizure termination.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biology
Samuel J. Barnes, Georg B. Keller, Tara Keck
Summary: This study used two-photon imaging to observe changes in neuronal synaptic calcium signals in awake mice, revealing that spines correlated with intrinsic network activity undergo TNF-α-dependent homeostatic enhancement, while spines responsive to sensory stimulation do not. Following sensory deprivation, global sensory-evoked responses increased, despite identified sensory inputs not strengthening.
Article
Psychiatry
Ling Chen, Wan-Kun Gong, Cui-ping Yang, Chan-Chan Shao, Ning-Ning Song, Jia-Yin Chen, Li-Qiang Zhou, Kun-Shan Zhang, Siguang Li, Zhili Huang, Gal Richter-Levin, Lin Xu, Yu-Qiang Ding
Summary: The study suggests that PTEN may serve as an alternative approach for regulating 5-HT neuron activity in the brain and depressive behaviors, compared to selective serotonin reuptake inhibitors (SSRIs) commonly used in depression treatment. Elevated levels of PTEN in 5-HT neurons can induce depressive behaviors in mice, while selective deletion of Pten leads to structural plasticity in these neurons.
TRANSLATIONAL PSYCHIATRY
(2021)
Article
Engineering, Electrical & Electronic
Lindong Wu, Zongwei Wang, Lin Bao, Zhizhen Yu, Qingyu Chen, Yaotian Ling, Yabo Qin, Shengyu Bao, Zhuoya Chen, Guandong Bai, Yimao Cai, John Robertson, Ru Huang
Summary: This article examines the application of neuronal intrinsic plasticity in neural networks. By adjusting the tunable load resistor and stimulus intensity, the oscillatory threshold of artificial neurons is studied. The results demonstrate that neuronal intrinsic plasticity can improve the performance of neural networks.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2022)
Article
Biochemistry & Molecular Biology
Eisuke Ichise, Tomohiro Chiyonobu, Mitsuru Ishikawa, Yasuyoshi Tanaka, Mami Shibata, Takenori Tozawa, Yoshihiro Taura, Satoshi Yamashita, Michiko Yoshida, Masafumi Morimoto, Norimichi Higurashi, Toshiyuki Yamamoto, Hideyuki Okano, Shinichi Hirose
Summary: This study generated GABAergic neurons from STXBP1-E patient-derived iPSCs and demonstrated slight reduction in STXBP1 protein levels, dysfunctional maturation, and specific dysregulation of genes in these neurons, highlighting the crucial role of GABAergic dysfunction in STXBP1-E pathogenesis.
HUMAN MOLECULAR GENETICS
(2021)
Article
Neurosciences
Adriana L. Hotz, Ahmed Jamali, Nicolas N. Rieser, Stephanie Niklaus, Ecem Aydin, Sverre Myren-Svelstad, Laetitia Lalla, Nathalie Jurisch-Yaksi, Emre Yaksi, Stephan C. F. Neuhauss
Summary: The loss of function in the astroglia predominant eaat2a gene in zebrafish leads to altered neuronal and astroglial networks, resulting in epileptic seizures and increased extracellular glutamate levels. Interestingly, while hyperexcitability is observed during seizures, basal neuronal and astroglial activity is reduced, leading to decreased overall locomotion in eaat2a(-/-) mutant animals.
Review
Pharmacology & Pharmacy
Valerie Crepel, Christophe Mulle
CURRENT OPINION IN PHARMACOLOGY
(2015)
Article
Neurosciences
Anders V. Petersen, Camilla S. Jensen, Valerie Crepel, Mathias Falkerslev, Jean-Francois Perrier
FRONTIERS IN CELLULAR NEUROSCIENCE
(2017)
Article
Clinical Neurology
Ludovic Franck Petit, Marion Jalabert, Emmanuelle Buhler, Arnaud Malvache, Angelique Peret, Yoann Chauvin, Francoise Watrin, Alfonso Represa, Jean-Bernard Manent
ANNALS OF NEUROLOGY
(2014)
Article
Neurosciences
Giulia Bonetto, Bruno Hivert, Laurence Goutebroze, Domna Karagogeos, Valerie Crepel, Catherine Faivre-Sarrailh
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Article
Neurosciences
Claire Pleau, Angelique Peret, Edouard Pearlstein, Thomas Scalfati, Alexandre Vigier, Geoffrey Marti, Francois J. Michel, Thomas Marissal, Valerie Crepel
Summary: Research has found that DGCs recruited in the home cage condition are mature neurons with longer dendritic trees and lower excitability. The higher GABA(A) receptor-mediated shunting inhibition contributes to the lower excitability of DGCs activated in the home environment.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Alexandre Vigier, Nicolas Partouche, Francois J. Michel, Valerie Crepel, Thomas Marissal
Summary: Researchers have developed an improved protocol that increases the survival rate of the pilocarpine mouse model of temporal lobe epilepsy by injecting a moderate dose of caffeine during the induction phase. This new protocol also involves using young male mice and a refined scoring system.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Multidisciplinary Sciences
Shu Ho, Rebecca Lajaunie, Marion Lerat, Mickael Le, Valerie Crepel, Karine Loulier, Jean Livet, Jean-Pierre Kessler, Paikan Marcaggi
Summary: The majority of synapses in the cerebellum of mature mice are silent, playing a crucial role in optimizing information storage capacity and reliability.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Clinical Neurology
Celine Boileau, Severine Deforges, Angelique Peret, Didier Scavarda, Fabrice Bartolomei, April Giles, Nicolas Partouche, Justine Gautron, Julio Viotti, Haley Janowitz, Guillaume Penchet, Cecile Marchal, Stanislas Lagarde, Agnes Trebuchon, Nathalie Villeneuve, Julie Rumi, Thomas Marissal, Roustem Khazipov, Ilgam Khalilov, Fanny Martineau, Marine Marechal, Anne Lepine, Mathieu Milh, Dominique Figarella-Branger, Etienne Dougy, Soutsakhone Tong, Romain Appay, Stephane Baudouin, Andrew Mercer, Jared B. Smith, Olivier Danos, Richard Porter, Christophe Mulle, Valerie Crepel
Summary: This study aims to reduce chronic epileptic discharges in TLE patients by downregulating KARs in the hippocampus using gene therapy. The results demonstrate that gene silencing strategy can inhibit chronic seizures in a mouse TLE model and IEDs in cultured slices derived from TLE patients.
ANNALS OF NEUROLOGY
(2023)
Article
Neurosciences
Lucas Goirand-Lopez, Marie Moulinier, Alexandre Vigier, Celine Boileau, Alan Carleton, Sarah F. Muldoon, Thomas Marissal, Valerie Crepel
Summary: Temporal lobe epilepsy (TLE) is a common form of epilepsy in adults, characterized by aberrant epileptogenic network formed by mossy fiber sprouting. Kainate receptors (KARs) play a prominent role in this network by facilitating the recruitment of neurons into coactive cell assemblies, contributing to the occurrence of epileptiform network events.
NEUROBIOLOGY OF DISEASE
(2023)
Meeting Abstract
Neurosciences
D. Pinatel, E. Pearlstein, D. Karagogeos, V. Crepel, D. Meijer, C. Faivre-Sarrailh
Article
Clinical Neurology
Nazim Kourdougli, Christophe Pellegrino, Juho-Matti Renko, Stanislav Khirug, Genevieve Chazal, Tiina-Kaisa Kukko-Lukjanov, Sari E. Lauri, Jean-Luc Gaiarsa, Liang Zhou, Angelique Peret, Eero Castren, Raimo K. Tuominen, Valerie Crepel, Claudio Rivera
ANNALS OF NEUROLOGY
(2017)
Article
Neurosciences
David W. Ouedraogo, Pierre-Pascal Lenck-Santini, Geoffrey Marti, David Robbe, Valerie Crepel, Jerome Epsztein